中药石菖蒲及其主要成分α-细辛醚对癫痫幼鼠惊厥行为及脑电图的影响

doi:吉林省科技厅自然科学基金资助课题（200304

中药石菖蒲及其主要成分α-细辛醚对癫痫幼鼠惊厥行为及脑电图的影响

李树蕾¹，杨立彬^2*，黄艳智³，梁建民²，王宇虹⁴，张淑琴⁵

1. 吉林大学基础医学院组织学与胚胎学教研室，吉林长春130021；2. 吉林大学第一医院儿内科，吉林长春130021；3. 长春市儿童医院，吉林长春130051；4. 哈尔滨医科大学附属第一临床医院神经科，黑龙江哈尔滨135001

收稿日期:2004-12-14 修回日期:1900-01-01 出版日期:2006-01-28 发布日期:2006-01-28
通讯作者: 杨立彬

Effect of acrous gramineus and its component α-asaroneon eclampsia and lectronecephalogram changesin young epileptic rats

LI Shu-lei¹, YANG Li-bin^2*, HUANG Yan-zhi³,LIANG Jian-min², WANG Yu-hong⁴, ZHANG Shu-qin⁵

1. Department of Histology and Embryology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China; 2. Department of Pediatrics, First Hospital, Jilin University,Changchun 130021,China; 3. Children Hospital of Changchun City,Changchun 130051,China; 4. Department of Neurology, First Clinical Hospital, Haerbin University of Medical Sciences,Haerbin 135001,China; 5. Department of Neurology, First Hospital, Jilin University, Changchun 130021, China

Received:2004-12-14 Revised:1900-01-01 Online:2006-01-28 Published:2006-01-28
Contact: YANG Li-bin

摘要/Abstract

摘要： 目的：观察中药石菖蒲及其主要成分α-细辛醚对戊四氮（PTZ）诱发的实验性癫痫幼鼠惊厥行为和额叶皮层放电的影响。方法：Wistar幼鼠随机分为正常对照组（N组）、致痫对照组（A组）、苯巴比妥钠组(B 组）、石菖蒲组（C组）和α-细辛醚组（D组）。除正常对照组外，各组幼鼠腹腔注射PTZ 60 mg·kg^-1建立癫痫动物模型。正常对照组和致痫对照组均给予生理盐水0.5 mL灌胃，其余各组分别给予苯巴比妥钠18 mg·kg^-1、石菖蒲2.35g·kg^-1、α-细辛醚29 mg·kg^-1灌胃。给药7 d后，除正常对照组外，各组幼鼠再次腹腔注射PTZ 60 mg·kg^-1，观察动物行为的改变，同时利用脑立体定位仪记录各组癫痫幼鼠脑部单侧额叶皮质的脑电变化。结果：①动物行为学观察显示，与致痫对照组比较，药物治疗组幼鼠癫痫发作的潜伏期延长（P<0.05），2 h强直-阵挛发作的次数减少（P<0.05），发作持续时间缩短（P<0.05）。α-细辛醚、石菖蒲的上述作用较苯巴比妥钠弱（P<0.05）。②脑电图结果显示，与致痫对照组比较，药物治疗组幼鼠额叶皮质痫性波发生潜伏期延长（P<0.05），2 h内异常放电频率下降，脑电波波幅下降（P<0.05）。α-细辛醚与石菖蒲对动物脑电的影响较苯巴比妥钠显著（P<0.05）。结论：石菖蒲及α-细辛醚均能有效抑制戊四氮诱发的幼鼠癫痫发作和额叶皮层的异常放电。

关键词: 石菖蒲, 药理学, α-细辛醚, 惊厥, 脑电图

Abstract: Objective To observe the effect of acrous g ramineus and its main component α-asarone on eclamptic behaviors and frontal lobe discharge in the young rats with experimental epilepsy induced by pentylenetetrazol (PTZ).Methods Young Wistar rats were divided into five groups randomly including normal control (N), epileptic control (A), phenobarbital sodium (B) , acrous gramineus (C), α-asarone （D）. Except normal control, other animals were ip. PTZ 60 mg·kg^-1 to found epileptic animal models. Rats in both normal control and epileptic control were ip. saline 0.5 mL, and other groups were ip. phenobarbital sodium 18 mg·kg^-1, acrous gramineus 2.35 g·kg^-1, α-asarone 29 mg·kg^-1, respectively. Expect normal control, other rats were ip. PTZ 60 mg·kg^{-1 again after they were treated for 7 d. Using brain solid positioner, the changes
of unilateral frontal cortex electroencephalogram (EEG) in young rats were recorded as well as their ecliptic behaviors were observed. Results ①Showed as animals′ behaviors, in treated groups, the latent period of paroxysm was delayed significantly (P<0.05), the number of titanic-cloni
c seizures was decreased obviously during 2 h (P<0.05), the mean duration of seizures was reduced compared with epileptic control (P<0.05). The functions of acrous gramineus and α-asarone mentioned above were weaker than phenobarbital sodium (P<0.05). ②Showed as EEG, in treated groups, the latent of
epileptic brain wave in frontal cortex was elongated (P<0.05), both the frequency of abnormal discharge in 2 h and the amplitude of brain wave were descended compared with epileptic control (P<0.05). The influences of acrous gramineus and α-asarone on EGG were more significant than phenobarbital sodium (P<
0.05). Conclusion Both acrous gramineus and α-asarone can suppress seizure and abnormal discharge of frontal cortex induced by pentylenetetrazol in young rats availably.}

Key words: acrous gramineus, pharmacology, α-asarone, convulsions, electroencephalogram

中图分类号:

R-332

李树蕾，杨立彬，黄艳智，梁建民，王宇虹，张淑琴. 中药石菖蒲及其主要成分α-细辛醚对癫痫幼鼠惊厥行为及脑电图的影响[J]. J4, 2006, 32(1): 74-4.

LI Shu-lei, YANG Li-bin, HUANG Yan-zhi,LIANG Jian-min, WANG Yu-hong, ZHANG Shu-qin. Effect of acrous gramineus and its component α-asaroneon eclampsia and lectronecephalogram changesin young epileptic rats[J]. J4, 2006, 32(1): 74-4.

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