关键词: 心肌缺血, 药物疗法, 再灌注损伤, 药物疗法, 天冬氨酸氨基转移酶类, 血液, 乳酸脱氢酶, 血液 , 超氧化物歧化酶, 血液, 自由基

Abstract: Objective To observe the protective effects of Diemailing Injecti on (DMLI) on myocardial ischemia-reperfusion injury in rats. Methods The myocardial ischemia-reperfusion model was induced by 30 min left a nterior descending coronary occulusion and 24 h reperfusion in open-chest anesthetized rats. The changes of aspartate aminotransferase(AST), lactate dehydrogenase(LDH), MB isoenzyme of creatine kinase (CK-MB), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) contents in serum, and prostacycline(PGI₂) and thromboxane A₂ (TXA₂) levels in plasma were determined. Results In rats treated by DMLI (with dose of 2.5,5 and 10 mL·kg^-1 i.v. at 30 min after coronary occulusion),the myocardial ischemia size (MIS) was significantly reduced, the AST，LDH and CK-MB activities in serum and the TXA₂ leve l in plasma were declined, while PGI₂ level in plasma and PGI_{2/TXA2 ratio were increased significantly. In addition, the LPO content in serum declined, SOD and GSH-Px activities in serum were increased markedly. Conclusion DMLI has protective effects on myocardial ischemia-reperfusion injury through improving free radicals metabolism, decreasing TXA2 level in plasma, increasing PGI2 level in plasma and PGI2/TXA2 ratio.}

Key words: myocardial ischemia, drug therapy, myocardial reperfusion injury, drug therapy, aspartate aminotransferases, bloo d, lactate dehydrogenase, blood, superoxide dismutase, blood, free radicals