关键词: 药理学, 环磷酰胺, 淋巴细胞增殖, 抑制, 遗传

Abstract: Objective To study the effect of anti-mutation of Chinese medicinal herb Polygala tenuifolia willd on T lymphocyte proliferation in spleen. Methods The micronucleus test of mouse bone marrow cell (MNT):thirty mice were divided into six groups (n=5)， negative control（NS）,cyclophosphamide group（CP 3.0 mg·kg^-1），Polygala tenuifolia willd antimutagenesis groups（Polygala tenuifolia willd with dosage of 0.5,1.0,2.0,4.0 g·kg^-1+ CP 30 mg·kg^-1）. The improved method was used to detect the micro-nuclei frequency. Lymphocyte transformation test: twenty-four mice were divided into four groups (n=6)， saline control ,CP 　control（3.0 mg·kg^-1）,Polygala tenuifolia willd （2.0 g·kg^-1），Polygala tenuifolia willd+CP（2.0 g·kg^-1 Polygala tenuifolia willd+CP 30 mg·kg^-1） group. MTT assay was used to calculate the stimulation index(SI). Results The micro-nuclei frequency was significant difference between Polygala tenuifolia willd antimutagenesis groups and CP groups (P<0.05)；the SI in saline control,Polygala tenuifolia willd,Polygala tenuifolia willd+CP,CP groups were 2.11±0.13,2.37±0.18,1.97±0.06 and 1.34±0.19,resp ectively, the difference of SI was significant between Polygala tenuifolia willd and saline control groups（P<0.05）. The difference of SI was significant between Polygala tenuifolia willd+CP and CP groups（P<0.05）. Conclusion Polygala tenuifolia willd may protect genetic material from injury caused by CP, namely,showing effective antimutagenesis and ye t Polygala tenuifolia.willd may increase the reactivity of mouse spleen cells to ConA and could improve reactivity reduction induced by CP, namely Polygala tenuifolia willd could enhance lymphocyte function.

Key words: pharmacology, cyclophosphamide, lymphocyte proliferation, suppression, genetic