关键词: HL-60细胞, 细胞增殖, 细胞凋亡

Abstract: Objective To observe the effects of fluvastatin on proliferation and apoptosis of HL-60 cells,and to offer the theoretical evidence for tumor treatment.Methods HL-60 cells were divided into: fluvastatin groups (0.5,5.0,10.0 and 20.0 μmol•L^-1),HL-60 control group,positive control group(treated with 10.0 μmol•L^-1ATRA).　The live cell number was counted for cell proliferation assay.The growth inhibitory rate of HL-60 cells was detected using CCK-8 kit.The cell cycle distribution and apoptotic rate were measured using flow cytometry assay.Results Compared with control group,after HL-60 cells were treated with 0.5,5.0,10.0 and 20.0 μmol•L^-1of fluvastatin for 1-4 d,the number of live cells decreased in different level(P<0.01),and in a dose- and time-dependent manner .After HL-60 cells were treated with fluvastatin for 2-72 h,the inhibitory rate of cell growth was markedly elevated,and the inhibitory rate further increased along with doses of fluvastatin and action time.The progressively increased cell growth inhibition was found in 10.0 and 20.0 μmol•L^-1 fluvastatin groups after treated for 48 and 72 h compared with the same concentration after treated for 2 h(P<0.01).Flow cytometry assays exhibited that the percentage of G₀/G₁ phase cells elevated (P<0.05),the percentages of S phase cells descended significantly,and the apoptotic rate increased after HL-60 cells were exposed to 20 μmol•L^-1 fluvastatin for 48 h,compared with control group(P<0.01).Fluvastatin-induced cell cycle distribution and apoptosis were reversed by coexposure to fluvastatin and mevalonate,Conclusion Fluvastatin inhibits proliferation and induces apoptosis of HL-60 cells,and these effects are mediated through mevalonate pathway.

Key words: HL-60 cell, cell proliferation, apoptosis