关键词: 消炎药, 非甾类, 喉肿瘤, 一氧化氮合酶

Abstract: Objective To investigate the effects of indomethacin on proliferation and inducible nitric oxide synthase(iNOS) activity of laryngeal cancer cell Hep-2 to provid theoretical evidence for precaution and healing of laryngeal cancer. Methods Hep-2 cells were exposed to indomethacin at concentrations of 30，60,125,125 μmol•L^-1 and cultivated for 24 and 48 h;MTT and trypan blue exclusion experiments were used to determine cell proliferation and cell viability respectively, and compared with control group. Hep-2 cells were treated with indomethacin（125,250,500 μmol•L^-1） and lipopolysacchairde(LPS),10 μmol•L^-1) for 16 h,control group with LPS alone was set up. Enzymic method and spectrophotometric method were used to determine the activity of iNOS in cultured supernatant. Results Compared with control group,as the increase of dosage, the inhibitory rates of proliferation of cancer cell Hep-2 in different concentrations of indomethacin groups increased significantly （P<0.05）. While the identical concentrations of indomethacin were administered for 24 and 48 h, the inhibitory rates of proliferation of cancer cell Hep-2 decreased significantly（P<0.05）. Compared with control group,as the increase of dosage, the inhibition of cell viability of cancer cell Hep-2 in different concentrations of indomethacin groups decreased significantly （P<0.05）; Compared with LPS control group, indomethacin (125, 250, and 500 μmol•L^-1) decreased the LPS-induced iNOS activity significantly（P<0.05）in the supernatant. Conclusion Indomethacin, within the concentration range of 30-250 μmol•L^-1，has strong inhibitory effect on Hep-2 cell proliferation and viability and may decrease LPS-induced cell iNOS activity significantly .

Key words: anti-inflammatory agents, non-steroidal, laryngeal neoplasms, nitric oxide synthase