关键词: 2型糖尿病；抵抗素；基因多态性；大血管病变；聚合酶链反应

Abstract:

Abstract:Objective To study the allelic and genotypic frequence distribution of RETN -420C/G single nucleotide polymorphisms (SNPs) and its relationship with type 2 diabetes mellitus (T2DM) complicated with macroangiopathy in Han population of northeast China.  Methods The genotypes and their frequencies of a total of 180 cases,including 60 cases of T2DM complicated with macroangiopathy,60 cases of simple T2DM and 60 cases of normal control(ND),were measured by PCR-RFLP. Results The allelic frequencies and simple CC/GC+GG genotype inT2DM complicated with macroangiopathy group were significant different compared with  ND group(P<0.01). The allelic frequencies and CC/GC+GG genotype in T2DM comlicated with macroangiopathy  group were significant different compared with T2DM group(P<0.05). The GC+GG genotype in T2DM complicated with macroangiopathy group was significantly higher than that in T2DM group(P<0.05). Conclusion The RETN-420C/G polymorphism has a correlation with the occurrence of T2DM complicated with macroangiopathy in Han population of northeast China.

Key words:  type 2 diabetes mellites;resistin;gene polymorphism;macroangiopathy;polymerase chain reaction