蒺藜皂苷对成年豚鼠心室肌细胞钾电流的影响

doi:国家自然科学基金资助课题（30472020，3067

蒺藜皂苷对成年豚鼠心室肌细胞钾电流的影响

李红，张爽^*，石卓，杨世杰

(吉林大学基础医学院药理学教研室，吉林长春130021)

收稿日期:2008-04-14 修回日期:1900-01-01 出版日期:2008-11-28 发布日期:2008-11-28
通讯作者: 杨世杰

Effect of GSTT on potassium current of ischemic cardiocytes in guinea pigs

LI Hong,ZHANG Shuang^*,SHI Zhuo,YANG Shi-jie

（Department of Pharmacology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China）

Received:2008-04-14 Revised:1900-01-01 Online:2008-11-28 Published:2008-11-28
Contact: YANG Shi-jie

摘要/Abstract

摘要： 目的：研究蒺藜皂苷（GSTT）对成年豚鼠心室肌细胞动作电位和钾通道的影响，初步探讨其抗心律失常的作用及机制。方法：分离豚鼠心肌细胞，建立缺氧模型，分为正常对照组、模型组及GSTT10和30 mg•L^-1组，采用全细胞膜片钳记录各组成年豚鼠单个心肌细胞的电位变化及测定钾通道电流。结果：与模型组比较，GSTT 30　mg•L^-1组缺氧细胞动作电位平台期即动作电位复极时程（50％和90％）延长 (P<0.01)。将保持电位控制在－40 mV，测试电位从－100 mV到+100 mV，阶跃命令为+10 mV，时程300 ms。正常对照组豚鼠心室肌细胞钾通道处于关闭状态， GSTT 10和30 mg•L^-1剂量组在+80 mV时其峰值电流分别为（1.19±0.20）和（1.15±0.10）nA，与模型组［（1.50±0.20）nA］比较差异有显著性（P<0.05和P<0.01）。结论： GSTT可阻碍缺氧导致的钾电流增加，即减少缺氧引起的钾离子外流，改善胞内钾离子丢失状态，显著延长缺氧细胞动作电位复极时程，延长有效不应期，从而改善缺氧及钾丢失导致的心肌细胞电生理异常，减少心律失常的发生，保护心肌细胞。

关键词: 动作电位时程, 钾电流

Abstract: To Study the effect of Gross Saponins of Tribulus Terrestris on active potential and potassium current of ischemic cardiocytes in guinea pigs， and approach its initial anti-arrhythmia effect and mechanism.Methods The cardiocytes of adult guinea pigs were isolated and hypoxia models were set up,the effects of injected GSTT on the cellular membrane active potential and electricity flow of potassium channel were recorded with whole cell patchclamp system. Results Compared with model group,the active plateau period(50% and 90%) of myocytes in GSTT 30 mg•L^-1 group prolonged(P<0.01).When the electric potential at －40 mV，the test electric potential was from －100 mV to +100 mV，step order at +10 mV，and duration at 300 ms.The myocyte potassium ion in control group closed in this condition.The peak currents in GSTT 10 and 30 mg•L^-1 groups were （1.19±0.20）and（1.15±0.1）nA，,and the difference was significant compared with model group（1.50 nA±0.20 nA］ (P<0.05 and P<0.01). Conclusion GSTT could extend action potential duration,prevent potassium current,decrease the cellular membrane electricity alternations so that it could protect the cardiac myocytes from hypoxia damage.

Key words: action potential duration, potassium current

中图分类号:

R285.5

李红，张爽，石卓，杨世杰. 蒺藜皂苷对成年豚鼠心室肌细胞钾电流的影响[J]. J4, 2008, 34(6): 927-930.

LI Hong,ZHANG Shuang,SHI Zhuo,YANG Shi-jie. Effect of GSTT on potassium current of ischemic cardiocytes in guinea pigs[J]. J4, 2008, 34(6): 927-930.