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尾加压素Ⅱ对体外高糖培养大鼠肾小球系膜细胞增殖的影响及其意义

赵 岩1*，李 才1，林风武2，田 琳1，李相军1，石 艳1，苗春生1   

  1. （1.吉林大学药学院实验药理与毒理学教研室，吉林 长春130021;2.吉林大学中日联谊医院胸外科，吉林 长春 130033）
  • 收稿日期:2008-05-12 修回日期:1900-01-01 出版日期:2008-11-28 发布日期:2008-11-28
  • 通讯作者: 李 才

Effect of urotensin Ⅱ on proliferation of rat mesangial cells cultivated with high glucose culture medium in vitro and its significances

ZHAO Yan1*，LI Cai1，LIN Feng-wu2，TIAN Lin1,LI Xiang-jun,SHI Yan1,MIAO Chun-sheng1   

  1. （1.Department of Experimental Pharmacology and Toxicology，School of Pharmacy，Jilin University， Changchun 130021，China；2.Department of Thoracic Surgery，China-Japan Union Hospital，Jilin University，Changchun 130033，China）
  • Received:2008-05-12 Revised:1900-01-01 Online:2008-11-28 Published:2008-11-28
  • Contact: LI Cai

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摘要： 目的：探讨尾加压素Ⅱ（UⅡ）对体外高糖培养的大鼠肾小球系膜细胞（MC）增殖影响的机制及意义。方法：体外高糖培养大鼠肾MC，分为5组：对照组及不同浓度UⅡ（10-7、10-8、10-9和10-10 mol•L-1）组，37℃孵育24 h，用流式细胞术分析处于细胞周期中S期的MC比例，MTT比色法检测细胞增殖率，BrdU-ELISA法测定细胞培养上清中BrdU含量。结果：MTT实验，UⅡ10-9及10-8 mol•L-1组细胞增殖率明显高于对照组（P<0.05），UⅡ10-10及10-7 mol•L-1组细胞增殖率与对照组比较差异无显著性（P>0.05）；流式细胞术实验，UⅡ10-9及10-8 mol•L-1组MC细胞周期中S期比例较对照组明显增加（P<0.05），UⅡ10-10及10-7 mol•L-1组MC细胞周期中S期的比例与对照组比较差异无显著性（P>0.05）；BrdU-ELISA实验，UⅡ10-9及10-8 mol•L-1组MC培养上清中BrdU含量明显低于对照组（P<0.05），UⅡ10-10及10-7 mol•L-1组MC培养上清中BrdU含量与对照组比较差异无显著性（P>0.05）。结论：10-9及10-8 mol•L-1浓度的UⅡ对体外高糖培养的大鼠肾小球MC具有明显促增殖作用。

关键词: 肾小球系膜细胞, 细胞培养, 细胞增殖

Abstract: To investigate the effect of urotensin Ⅱ (UⅡ) on proliferation of the rat mesangial cells (MC) cultivated with high glucose culture medium in vitro and explore its mechanism and significances. Methods The rat kidney MC cultivated with high glucose in vitro were divided into five groups: control group and different concentrations of UⅡ(10-7,10-8,10-9,10-10mol•L-1)groups,incubated at 37℃ for 24 h,the proliferation rates in various groups were determined by MTT assay，the ratios of MC in S phase in various groups were detected by flow cytometry，the BrdU contents in various groups were determined by BrdU incorporation method.Results In MTT experiment,the MC proliferation rates in UⅡ10-9 and UⅡ10-8 mol•L-1 groups were significantly higher than that in control group (P<0.05),and the results in UⅡ 10-10 and UⅡ 10-7mol•L-1 groups had no significant change compared with control group (P> 0.05); In flow cytometry experiment,the ratios of MC in S phase in UⅡ 10-9 and UⅡ 10-8 mol•L-1 groups were increased significantly compared with control group (P< 0.05),and the results in UⅡ 10-10 and UⅡ 10-7 mol•L-1 groups had no significant change compared with control group (P> 0.05); In BrdU-ELISA test ，BrdU contents in UⅡ 10-9 and UⅡ 10-8 mol•L-1 groups were significantly lower than that in control group (P< 0.05),while the results in UⅡ 10-10 and UⅡ 10-7 mol•L-1 groups had no significant change compared with control group (P> 0.05). Conclusion UⅡ at the concentrations of 10-9 and 10-8 mol•L-1 have obvious role in promoting proliferation of rat MC.

Key words: mesangial cells, cell culture , cell proliferation

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  • R587.1
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