多烯磷脂对青年鼠海马CA3区神经元突触可塑性的影响

J4 ›› 2009, Vol. 35 ›› Issue (3): 419-422.

多烯磷脂对青年鼠海马CA3区神经元突触可塑性的影响

安思训¹| 陈晓丽²|何欣¹| 董志恒¹

1.北华大学基础医学院解剖学教研室| 吉林吉林132013；2.北华大学附属医院循环二科 |吉林吉林 132001

收稿日期:2008-09-19 出版日期:2009-05-28 发布日期:2009-08-14
通讯作者: 何欣 E-mail:hexin8040@163.com
作者简介:安思训(1969-), 男|吉林省通榆县人|副教授|主要从事临床应用解剖学研究。
基金资助:
吉林省科技厅科研基金资助课题(200705396)

Effect of polyene phosphatidyl on synaptic plasticity in |region of hippocampus CA3 of young rats

1.Department |of Anatomy, School of Basic Medical Sciences, Beihua University,Jillin 132013, China|2.Second Department of Circulatory System Diseases, Affiliated Hospital, |Beihua University| Jilin 132001, China

Received:2008-09-19 Online:2009-05-28 Published:2009-08-14

摘要/Abstract

摘要：

目的：观察青年大鼠海马CA3区神经元突触结构变化及大鼠突触老龄性改变，探讨多烯磷脂提高学习记忆功能的形态学基础及对突触可塑性的影响。方法： 5月龄Wistar大鼠20只，雌雄各半，随机分为多烯磷脂组和对照组，每组10只。喂养4周后进行水迷宫训练，连续训练1周。用免疫组化和MOTAC图像分析系统检测大鼠海马CA3区突触素（SYN）的免疫表达，用电镜观察海马CA3区突触变化。选取老龄鼠（24~26月龄）10只，雌雄各半，相同环境正常饲养，透射电镜观察老龄鼠海马CA3区神经元超微结构改变。 结果：多烯磷脂组海马CA3区SYN表达 (0.430 0±0.022 4)明显高于对照组(0.356 7±0.0209)（P<0.05）。电镜下观察，多烯磷脂组海马CA3区突触数密度［(102.69±8.96).μm^-3］和活性区膜面积［(0.066±0.010) μm²/μm³］　高于对照组［(82.89±6.52) .μm^-3和(0.046±0.006) μm²/μm³］（P<0.05）。青年大鼠突触结构完整，前后膜间隙清晰，棘器清晰活跃；而老龄鼠突触结构不完整，前后膜融合，间隙模糊，扁平小囊增多并扩张，棘器变性。结论：多烯磷脂可明显改善大鼠海马CA3区SYN表达，增加突触活性区面积及突触数量，提高大鼠的空间辨别学习记忆能力。

关键词: 多烯磷脂, 海马CA3区, 突触, 突触素, 突触可塑性

Abstract:

Abstract:Objective To observe the structure changes of the synapse of the neurons in hippocampus CA3 of young rats and study the basis for the mechanism of polyene phosphatidyl in providing learning and memory and the effect on synaptic plasticity. Methods A total of 20 Wistar rats with 5 months were randomly divided into polyene phosphatidyl group and normal control group. Each group had 10 rats. After 4 weeks feeding, Water maze training was peformed in all the rats for 1 weeks. The immue expressions of synapsin (SYN) of the rats in polyene phosphatidyl group and control groups were observed with immunohistochemical method and analyzed by MOTAC imagine analyzing system. The change of synapse of hippocamal CA3 was observed with electron microscope. And the other 24 to 26 months rats were selected as aged group,and fed in the same condition. Moreover, the ultrastructures of hippocamal CA3 of aged rats were observed by transmission electron microscopy(TEM).Results The SYN in polyene phosphatidyl group(0.430 0±0.022 4) was higher than that in control group(0.3567±0.0209) (P<0.05). The density of SYN in polyene phosphatidy1 group was (102.69±8.96)•μm^-3 and the area of SYN membrane in the region of hippocamal CA3 was (0.066±0.010) μm²/μm³, which were higher than those in control group(82.89•μm^-3±6.52•μm^-3, .046μm²/μm³±0.006 μm²/μm³ ) (P<0.05). Under TEM the abundance of synapse had complete structure, the synapse cleft were clear and the spine apparatus were active. The synaptic vesicles in aged rats increased, the size was different, and accumulation presented. The spine apparatus were far away from postsynaptic membrane; the flat vesicles of the spine apparatus were expanded and increased in number.Conclusion Additive polyene phosphatidyl can improve the expression of SYN in the region of hippocamal CA3 and increase the density of SYN and the area of SYN membrane, and improve the special discrimination of learning and memory in young rats.

Key words: polyene phosphatidyl, hippocamal CA3, synapse, synaptophysia, synaptic plasticity

中图分类号:

R-332

安思训, 陈晓丽, 何欣, 董志恒. 多烯磷脂对青年鼠海马CA3区神经元突触可塑性的影响[J]. J4, 2009, 35(3): 419-422.

AN Sai-Xun, CHEN Xiao-Li, HE Xin, DONG Zhi-Heng. Effect of polyene phosphatidyl on synaptic plasticity in |region of hippocampus CA3 of young rats[J]. J4, 2009, 35(3): 419-422.

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