20 (s)-原人参二醇对体外培养的人前列腺癌PC3细胞凋亡的诱导作用及其机制

J4 ›› 2012, Vol. 38 ›› Issue (3): 482-485.

20 (s)-原人参二醇对体外培养的人前列腺癌PC3细胞凋亡的诱导作用及其机制

程宏¹|赵丽晶¹|鲁育铭¹|许多¹| 邵晨¹|赵丽娟¹| |董研²

1.吉林大学白求恩医学院病理生理学系,吉林长春 130021;2.美国杜兰大学细胞生物学教研室,美国新奥尔良LA70062

收稿日期:2011-10-29 出版日期:2012-05-28 发布日期:2012-05-28
通讯作者: 赵丽娟(Tel:0431-85619485， E-mail: titan2003@sina.com.cn)； 董妍(Tel :01-4007128899,E-mail : drdong71 @gmail.com) E-mail:titan2003@sina.com.cn;drdong71 @gmail.com
作者简介:程宏(1984-)|女|吉林省长春市人|在读医学硕士|主要从事肿瘤防治研究。
基金资助:
吉林省人民政府人才开发基金资助课题(2006JD02)

Inductive effect of 20 (s)-proto-panaxdiol on apoptosis of human prostate cancer |PC3 cells cultivated |in vitro and its mechanism

CHENG Hong¹,ZHAO Li-jing¹,LU Yu-ming¹,XU Duo¹,SHAO Chen¹,ZHAO Li-juan¹,DONG Yan²

1. Department of Pathophysiology,Norman Bethune College of Medicine,Jilin University,Changchun 130021,China;2. Department of Structural and Cellular Biology,Tulan University,New Orleans LA 70062,USA

Received:2011-10-29 Online:2012-05-28 Published:2012-05-28

摘要/Abstract

摘要：

目的：观察20 (s)-原人参二醇 (PPD) 对体外培养的人前列腺癌（PCa）PC3 细胞凋亡的作用，阐明其抗肿瘤作用机制。方法：将体外培养的PC3 细胞分为对照组及 20、30和40 μmol?L-1PPD组。采用PPD处理细胞48 h后，利用光学显微镜观察细胞形态学改变，MTT法检测细胞增殖抑制情况，采用吖啶橙/溴化乙锭（AO/EB）染色法检测细胞凋亡情况，并通过Western blotting方法检测 Bcl-2、Bax和γH2Ax蛋白的表达情况。结果：不同剂量PPD处理后，PC3细胞变圆回缩并出现碎片，AO/EB染色呈现不同程度的黄绿色；不同剂量PPD作用后，各组细胞增殖抑制率均显著升高，与对照组比较差异有统计学意义(P<0.05)；不同剂量PPD作用PC3细胞48 h后，Bcl-2蛋白表达明显下调(P<0.05)，Bax蛋白表达变化不明显，γH2Ax蛋白表达上调(P<0.05)。结论：PPD可诱导PC3细胞凋亡，其作用机制可能与 Bcl-2、Bax信号通路以及DNA断裂基因γH2Ax蛋白表达上调有关。

关键词: 20(s)-原人参二醇；前列腺肿瘤；PC3细胞；细胞凋亡

Abstract:

Objective
To study the effect of 20(s)-proto-panaxdiol ( PPD) on apoptosis of human prostate cancer PC3 cells cultivated in vitro,and to clarify the mechanism of its anticancer action.Methods The PC3 cells cultivated in vitro were divided into control, and 20,30,40 μmol/LPPD groups.The morphological changes of PC3 cells were observed under optical microscope after treated with PPD for 48 h.The proliferation inhibition of PC3 cells was detected by MTT method.The apoptosis of PC3 cells was detected by AO/EB staining and the expressions of Bax,Bcl-2 and γH2Ax proteins were analyzed by Western blotting.Results After treated with different doses of PPD,the PC3 cells were round,recovery,and broken.AO/EB staining found that the cells presented yellow-green.The inhibitory rates of proliferation of PC3 cells in various groups were increased compared with control group(P<0.05).The expression of Bcl-2 protein was down-regulated(P<0.05), the expression of Bax protein didn’t change significantly, and the expression of γH2Ax protein was up-regulated(P<0.05).Conclusion PPD may induce the apoptosis of PC3 cells,and its mechanism may be related to the down-regulation of Bcl-2 protein and the up-regulation of γH2Ax protein expression.

Key words: 20 (s)-protopanaxdaol;prostate neoplasma;PC3 cells;apoptosis

中图分类号:

R329.26

程宏|赵丽晶|鲁育铭|许多| 邵晨|赵丽娟| 董研. 20 (s)-原人参二醇对体外培养的人前列腺癌PC3细胞凋亡的诱导作用及其机制[J]. J4, 2012, 38(3): 482-485.

CHENG Hong,ZHAO Li-jing,LU Yu-ming,XU Duo,SHAO Chen,ZHAO Li-juan,DONG Yan. Inductive effect of 20 (s)-proto-panaxdiol on apoptosis of human prostate cancer |PC3 cells cultivated |in vitro and its mechanism[J]. J4, 2012, 38(3): 482-485.