成纤维细胞生长因子-21对体外诱导的非酒精性脂肪肝细胞模型脂代谢的影响

J4 ›› 2012, Vol. 38 ›› Issue (3): 477-481.

成纤维细胞生长因子-21对体外诱导的非酒精性脂肪肝细胞模型脂代谢的影响

刘敏¹|孔祥鑫¹|杨苹¹|陈玉斌¹|李校堃¹|王会岩^1,2

1.吉林农业大学生物反应器与药物开发教育部工程研究中心|吉林长春 130118； 2.吉林医药学院检验系|吉林吉林 132013

收稿日期:2011-12-01 出版日期:2012-05-28 发布日期:2012-05-28
通讯作者: 李校堃（Tel:0431-84533427,E-mail：xiaokunli@163.net）；王会岩（Tel:0431-84533427,E-mail：w_huiyan@yahoo.com.cn） E-mail:xiaokunli@163.net;w_huiyan@yahoo.com.cn
作者简介:刘敏（1984-）|女|吉林省长春市人|理学硕士|主要从事基因工程药物方面的研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（20116044）

Effect of fibroblast growth factor-21 |on lipid metabolism of nonalcoholic fatty liver disease cell model induced |in vitro

LIU Min¹,KONG Xiang-xin¹,YANG Ping¹,CHEN Yu-bin¹,LI Xiao-kun¹,WANG Hui-yan ^1,2

1. Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural |University, |Changchun 130118,China;2. Department of Medical Spection,Jilin Medical College,Jilin 132013,China

Received:2011-12-01 Online:2012-05-28 Published:2012-05-28

摘要/Abstract

摘要：

目的：探讨成纤维细胞生长因子-21(FGF-21)对体外诱导的非酒精性脂肪肝（NAFLD）细胞模型脂代谢的影响及其可能的作用机制。方法：采用MTT法筛选医用脂肪乳注射液使用浓度和FGF-21作用浓度，将体外诱导的NAFLD细胞分为对照组、模型组、脱离脂肪乳组和FGF-21治疗组，光学显微镜观察油红O染色情况，全自动生化仪检测NAFLD细胞内甘油三酯(TG)水平，免疫细胞化学方法观察细胞内肉毒碱脂酰转移酶-1(CPT-1)、过氧化氢酶体增殖物激活受体γ(PPARγ)及固醇调节元件结合蛋白-1c（SREBP-1c）的表达。结果：MTT法筛选出0.1%医用脂肪乳注射液作为建立体外诱导NAFLD细胞模型的最佳诱导浓度，与正常肝细胞株HL7702比较，模型组油红O染色后细胞浆内有大量被染成橘红色的脂滴，细胞内TG水平显著升高（P＜0.01），CPT-1蛋白表达减弱，PPARγ和SREBP-1c蛋白表达增加。MTT法筛选出0.5 mg/LFGF-21作为观察该药物对NAFLD细胞模型影响的作用浓度，与模型组比较，FGF-21处理组细胞内TG水平显著降低（P＜0.01），CPT-1蛋白表达增加，PPARγ和SREBP-1c蛋白表达减弱。结论：FGF-21处理能减轻NAFLD细胞中TG蓄积，并使HL7702细胞CPT-1蛋白表达增加，而PPARγ和SREBP-1c蛋白表达减少。

关键词: 成纤维细胞生长因子-21；非酒精性脂肪肝病；医用脂肪乳注射液

Abstract:

Objective To study the effect of fibroblast growth factor-21 (FGF-21)on the lipid metabolism of nonalcoholic fatty liver disease（NAFLD）cell model induced in vitro and its potential mechanism.Methods The concentrations of medical fat emulsion (MFE) and FGF-21 were selected by MTT assay.And the NAFLD cells were divided into control group,model group,leaving fat emulsion group and FGF-21 therapy group.Oil red O staining was used to observe lipid droplet under light microscope.The intracellular triacylglycerol (TG) levels in each group were observed by hol-automatic biochemistry.The expressions of carnitine palmitovl transferase-1 (CPT-1),peroxisome proliferator-activated receptor-gamma (PPARγ) and sterol regulatory element binding protein-1c (SREBP-1c) were determined by immunocytochemistry.Results The 0.1% medical fat emulsion (MFE) was chosen by MTT assay as the best concentration for the induction of NAFLD cells in vitro.The intracellular TG level of NAFLD cells was increased significantly compared with normal liver cells HL7702 (P＜0.01),and there was a large quantity of orange-red lipid droplet in cytoplasm of NAFLD cells;meanwhile,the expression of CPT-1 protein got lower,and the expressions of PPARγ and SREBP-1c proteins got higher.MTT assay showed the best management concentration of FGF-21 was 0.5 mg/L for setting up NAFLD-effect model,the intracellular TG level in FGF-21 group was decreased significantly compared with model group(P＜0.01);the expression level of CPT-1 protein was increased (P＜0.01),and the expressions of PPARγ and SREBP-1c proteins were decreased in FGF-21 group.Conclusion FGF-21 is helpful to relieve accumnlation of TG in NAFLD cells,and it decrease the expressions of PPARγ and SREBP-1c protein and increase the expression of CPT-1 protein in HL7702 cells.

Key words: fibroblast growth factor-21;nonalcoholic fatty liver disease;medical fat emulsion

中图分类号:

刘敏|孔祥鑫|杨苹|陈玉斌|李校堃|王会岩. 成纤维细胞生长因子-21对体外诱导的非酒精性脂肪肝细胞模型脂代谢的影响[J]. J4, 2012, 38(3): 477-481.

LIU Min,KONG Xiang-xin,YANG Ping,CHEN Yu-bin,LI Xiao-kun,WANG Hui-yan. Effect of fibroblast growth factor-21 |on lipid metabolism of nonalcoholic fatty liver disease cell model induced |in vitro[J]. J4, 2012, 38(3): 477-481.

[1]	周颖,高孟飞,朱慧勇. 间充质干细胞干性维持信号通路的研究进展[J]. 吉林大学学报(医学版), 2013, 39(3): 638-642.
[2]	李红，陈永浩，龚红君. 右美托咪定对肾癌根治术患者围术期炎性细胞因子和肾功能的影响[J]. 吉林大学学报(医学版), 2013, 39(3): 588-591.
[3]	李海波，刘行，孙美花,梁建民. 孕期至幼年期低水平铅暴露仔鼠海马锌离子平衡的变化及其发育期海马神经元的损伤[J]. 吉林大学学报(医学版), 2013, 39(3): 521-528.
[4]	谢波，沈梅，叶文鑫，舒彦，梁臻，张心男. 依帕司他联合行为疗法治疗女性糖尿病膀胱的临床疗效评价[J]. 吉林大学学报(医学版), 2013, 39(3): 597-600.
[5]	李辉,赵丽晶,杜宇,杨铁铮，李国峰,陈亮,支晨阳,李娜,周建华. 结肠安栓联合舒血宁对溃疡性结肠炎大鼠的治疗作用及其机制[J]. 吉林大学学报(医学版), 2013, 39(3): 488-493.
[6]	张洪长，王恩鹏，陈新，唐燕，王洪鑫，王炫策，许新. 人参皂苷Re对UVC辐射损伤细胞的保护作用[J]. 吉林大学学报(医学版), 2013, 39(3): 507-511.
[7]	武国东，王素侠，及志勇，张舒岩. 急性心力衰竭大鼠B型钠尿肽水平与肝功能的关系[J]. 吉林大学学报(医学版), 2013, 39(3): 517-520.
[8]	陈宣男，全首祯. NDM1基因常规PCR与荧光定量PCR检测方法的建立及其检测结果比较[J]. 吉林大学学报(医学版), 2013, 39(3): 625-629.
[9]	周超熙,于滨,赵斌,马志强,赵文河,于跃明. 直肠癌环周切缘病理学检测判断中低位直肠癌患者预后的临床价值[J]. 吉林大学学报(医学版), 2013, 39(3): 601-604.
[10]	周广宇，韩雪梅，金玲. 神经精神狼疮患者影像学表现与自身抗体水平和预后的关系[J]. 吉林大学学报(医学版), 2013, 39(3): 609-614.
[11]	周建英，田勇韩青,贺庆,程凯亮,李幼琼. 影像学三维重建技术在寰椎立体模型建立及性别鉴定中的应用[J]. 吉林大学学报(医学版), 2013, 39(3): 605-608.
[12]	沈煜，沈惠良，方秀统. 老年股骨颈骨折患者关节置换术后死亡危险因素分析[J]. 吉林大学学报(医学版), 2013, 39(3): 574-577.
[13]	王宗谦，卢新星. 终末期肾病患者抑郁程度与生存质量各个维度之间的关系[J]. 吉林大学学报(医学版), 2013, 39(3): 615-619.
[14]	李树春，王槐. 影响美托洛尔治疗效果的药物基因组学研究进展[J]. 吉林大学学报(医学版), 2013, 39(2): 423-426.
[15]	胡源，贾卉，姜瑞，李京，刘涛涛. 不同验光方法对中年患者屈光状态的检测及其临床意义[J]. 吉林大学学报(医学版), 2013, 39(2): 363-366.