关键词: 阿霉素, 热敏, 磁性

Abstract: To study the assembly and release of adriamycin in vitro using magnetic thermosensitive liposomes treated with magnetic field and pyrogenation. Methods ll samples were divided into six groups : 21, 33, 39, 42, 45℃ and control groups according to the temperature of water bath. Lucifer yellow iodoacetamide (LY) was used as a fluorescence marker to adriamycin which was encapsulated by magntic thermosensitive liposomes. Liposomes was localized under the action of magnetic field, and adriamycin was released through the use of water baths with LY used as a fluorescence marker to observe the assembly and release. Temperature-treated liposomes were mixed with CT-26 cells to measure the binding of the relea sed LY-marked adriamycin to cell surface. Results he magnetic thermosensitive adriamycin liposomes was assembled directionally under magnetic field. The onset of LY release occurred near 33℃, and reached plateau above 42℃ when 90% of the LY was released. The binding with the HT-29 had positive correlation with the release of the LY-marked adriamycin. Conclusion agnetic field can make the mag netic thermosensitive liposomes drug to assemble directionally, hyperthermia can control the release of drug from magnetic thermosensitive liposomes.

Key words: adriamycin, thermosensitive, magnetic