SPIO-shRNA分子探针注射不同时间点荷瘤裸鼠肿瘤区域组织信号变化及MRI最佳扫描时间

doi:10.13481/j.1671-587x.20160634

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (06): 1220-1225.doi: 10.13481/j.1671-587x.20160634

SPIO-shRNA分子探针注射不同时间点荷瘤裸鼠肿瘤区域组织信号变化及MRI最佳扫描时间

廖蕤堃, 邓小林, 曾丹妮, 吴晓凤, 文明

重庆医科大学附属第一医院放射科, 重庆 400016

收稿日期:2016-04-19 出版日期:2016-11-28 发布日期:2016-12-02
通讯作者: 文明,教授,硕士研究生导师(Tel:023-89012227,E-mail:13883669699@163.com) E-mail:13883669699@163.com
作者简介:廖蕤堃(1990-),男,重庆市人,在读医学硕士,主要从事分子影像探针制备和治疗方面的研究。
基金资助:
国家自然科学基金资助课题（81171366）；卫计委国家临床重点专科建设项目资助课题[（2013）544]

Changes of signals in tumor tissue of tumor-bearing nude mice after SPIO-shRNA molecular probe injection at different time points and optimal scanning time of MRI

LIAO Ruikun, DENG Xiaolin, ZENG Danni, WU Xiaofeng, WEN Ming

Department of Radiology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China

Received:2016-04-19 Online:2016-11-28 Published:2016-12-02

摘要/Abstract

摘要：

目的：探讨注射SPIO-shRNA分子探针不同时间点的荷瘤裸鼠肿瘤区域组织信号强度变化情况，阐明分子探针活体磁共振成像（MRI）机制和最佳扫描时间。方法：探针注射剂量为18mg·kg^-1；将30只BALB/c荷瘤裸鼠随机分为6组，每组5只，分别于探针注射前和注射后12、24、27、30和36 h等6个时间点行MRI检查，并测量裸鼠肿瘤组织及对侧肌肉组织在各时间点的信号强度变化；每次检查完成后立即处死裸鼠，取出肿瘤组织及肌肉组织行HE及普鲁士蓝染色，并在光学显微镜下观察裸鼠肿瘤组织的形态表现。结果：与注射前比较，探针注射后12、24和27 h裸鼠肿瘤组织T1WI信号强度均升高（P<0.05），12、24、27、30和36 h肿瘤组织T2WI和T2*GRE信号强度均降低（P < 0.05或P < 0.01）；探针注射后27 h肿瘤组织T1WI信号强度最高；探针注射后27 h肿瘤组织T2WI和T2*GRE信号强度低于注射后12、24、30和36 h（P<0.01）。与注射前比较，注射后24 h时裸鼠对侧肌肉组织信号强度降低（P<0.05）。HE染色，裸鼠肿瘤组织结构紊乱，细胞核异型性较多；普鲁士蓝染色，在注射后各时间点上均存在蓝染的Fe颗粒，注射后27 h时蓝染Fe颗粒最多。结论：SPIO-shRNA分子探针能成功靶向裸鼠肿瘤组织，且MRI最佳的扫描时间为探针注射后27 h。

关键词: 超顺磁性氧化铁, 分子探针, 磁共振成像, 扫描时间

Abstract:

Objective: To investigate the changes of signals in tumor tissue of the tumor-bearing nude mice after SPIO-shRNA molecular probe injection at different time points,and to clarify the mechanism and optimal scanning time of magnetic resonance imaging (MRI) by molecular probe in vivo. Methods: The injection dose of probe was 18 mg·kg^-1. A total of 30 BALB/c tumor-bearing nude mice were randomly divided into six groups(n=5). The mice in each group received MRI examination before and after injected with SPIO-shRNA molecular probe at different time points (12,24,27,30,and 36 h), and the changes of signal intensities in tumor tissue and controlateral muscle tissue of the nude mice were measured at different time points.Then the mice were sacrified after each examination,and the tumor and contralateral muscle tissues were obtained for HE and prussian blue staining, and the morphology of tumor tissue of the nude mice was observed under light microscope.Results: Compared with before injection,the signal intensities of T1WI in tumor tissue were increased at 12,24 and 27 h after injection of molecular probe(P<0.05),while the signal intensities of T2WI and T2*GRE were decreased at 12,24,27,30 and 36 h after injection of molecular probe(P < 0.05 or P < 0.01). The signal intensity of T2WI at 27 h after injection was the highest.However,the signal intensities of T2WI and T2*GRE at 27 h after injection were lower than those at 12,24,30, and 36 h(P<0.05). Compared with before injection,the signal intensity in contralteral muscle tissue of the nude mice at 24 h after injection was decreased(P<0.05). The HE staining results indicated that the structure of tumor tissue was abnormal and the unclear atypia was obvious.The prussian blue staining results showed that the blue-stained iron particles were distributed dispersedly at different time points, and the number of blue-stained iron particles was the most at 27 h after injection.Conclusion: The tumor tissue could be well labeled with the SPIO-shRNA molecular probes,and the optimal MRI scanning time is at 27 h after probe injection.

Key words: superparamagnetic iron oxide, molecular probe, magnetic resonance imaging, scanning time

中图分类号:

廖蕤堃, 邓小林, 曾丹妮, 吴晓凤, 文明. SPIO-shRNA分子探针注射不同时间点荷瘤裸鼠肿瘤区域组织信号变化及MRI最佳扫描时间[J]. 吉林大学学报(医学版), 2016, 42(06): 1220-1225.

LIAO Ruikun, DENG Xiaolin, ZENG Danni, WU Xiaofeng, WEN Ming. Changes of signals in tumor tissue of tumor-bearing nude mice after SPIO-shRNA molecular probe injection at different time points and optimal scanning time of MRI[J]. Journal of Jilin University Medicine Edition, 2016, 42(06): 1220-1225.

参考文献

[1] Starmans LW,Burdinski D,Haex NP,et al.Iron oxide nanoparticle-micelles (ION-micelles) for sensitive (molecular) magnetic particle imaging and magnetic resonance imaging[J].PLoS One,2013,8(2):e57335.
[2] 文曦琳,李易,葛晓东,等.卵巢癌特异性MR分子探针的制备[J].基础医学与临床,2014,34(11):1463-1467.
[3] 邓小林,葛晓东,吴晓凤,等.SPIO-shRNA双功能分子探针药动学及活体MR成像研究[J].药学学报,2015(10):1285-1289.
[4] 文曦琳,李易,葛晓东,等.SPIO-PLL-pshRNA分子探针转染卵巢癌SKOV3细胞及MR成像研究[J].重庆医科大学学报,2014(4):506-510.
[5] 葛晓东,李妹玲,邓小林,等.外加磁场对SPIO-shRNA分子探针转染卵巢癌SKOV3细胞的影响[J].中国医学科学院学报,2015,37(1):12-16.
[6] 温志鹏,刘海燕,文明,等.SPIO标记的c-erbB2反义探针用于活体荷瘤裸鼠MRI的最佳扫描浓度[J].南方医科大学学报,2013,33(4):496-501.
[7] 温志鹏,刘海燕,文明,等.SPIO标记的c-erbB2反义探针用于活体荷瘤裸鼠MR的最佳扫描时间探讨[J].四川大学学报:医学版,2013,44(5):708-712.
[8] 张松.CD105靶向金磁纳米粒的合成及其对乳腺癌血管生成的MRI和CT实验研究[D].重庆:第三军医大学,2014.
[9] Burnett J,Rossi J.RNA-based therapeutics:current progress and future prospects[J].Chem Biol,2012,19(1):60-71.
[10] Farra R,Grassi M,Grassi G,et al.Therapeutic potential of small interfering RNAs/micro interfering RNA in hepatocellular carcinoma[J].World J Gastroenterol,2015,21(30):8994-9001.
[11] Michele W,Wenlian Z,Mohammad H,et al.Magnetic resonance imaging contrast of iron oxide nanoparticles developed for hyperthermia is dominated by iron content[J].Int J Hyperthermia,2014,30(3):192-200.
[12] Shahbazigahrouei D,Abdolahi M.Detection of MUC1-expressing ovarian cancer by C595 monoclonal antibody-conjugated SPIONs using MR imaging.[J].Scientif World J,2013,2013(6):609151.
[13] Chen Z,Xiao EH,Kang Z,et al.In vitro and in vivo magnetic resonance imaging with chlorotoxin-conjugated superparamagnetic nanoprobes for targeting hepatocarcinoma[J].Oncol Rep,2016,35(5):3059-3067.
[14] Quan G,Du X,Huo T,et al.Targeted Molecular Imaging of Antigen OC183B2 in Ovarian Cancers Using MR Molecular Probes[J].Academ Radiol,2010,17(12):1468-1476.
[15] Casettari L,Vllasaliu D,Lam JKW,et al.Actively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging[J].Int J Nanomed,2012,7(2):3981-3989.
[16] Daryoush SG,Mohammad A.Superparamagnetic iron oxide-C595:Potential MR imaging contrast agents for ovarian cancer detection[J].J Med Phys,2013,38(4):198-204.
[17] 李易,文曦琳,孙红武,等.超顺磁性氧化铁(SPIO)与多聚赖氨酸(PLL)纳米粒的制备、结构验证及其空间结构表征[J].复旦学报:医学版,2014,41(3):309-314.

SPIO-shRNA分子探针注射不同时间点荷瘤裸鼠肿瘤区域组织信号变化及MRI最佳扫描时间

Changes of signals in tumor tissue of tumor-bearing nude mice after SPIO-shRNA molecular probe injection at different time points and optimal scanning time of MRI

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	章娉, 周佳, 唐焱, 刘红雨. 孕激素受体靶向聚乳酸-羟基乙酸-超顺磁性氧化铁分子探针的制备及其性能[J]. 吉林大学学报(医学版), 2018, 44(06): 1307-1311.
[2]	朱刚, 孙海斌, 汪浒, 徐刚. MRI、CT和病理检查对肢体脂肪肉瘤诊断价值的比较[J]. 吉林大学学报(医学版), 2017, 43(06): 1215-1219.
[3]	朱镇, 孙琪, 杨军星, 杨楠, 宋立杰, 王瑶, 赵楚翘, 刘志辉. 不同材料固定局部义齿患者行头颈部MRI检查的成像效果和安全性比较[J]. 吉林大学学报(医学版), 2017, 43(06): 1220-1225.
[4]	周宏, 刘进才, 谢佩含, 游咏, 罗光华, 杨娟, 卿伟鹏. 磁敏感序列分析和常规MRI检查对脑血管淀粉样变脑出血诊断价值比较[J]. 吉林大学学报(医学版), 2017, 43(05): 1042-1046.
[5]	宋志强, 沈海山, 沈俊, 吴建臣, 张明, 李然伟, 张继珍, 李胜文. MNU法诱导大鼠原位膀胱癌模型的建立及MRI的诊断价值[J]. 吉林大学学报(医学版), 2016, 42(04): 685-689.
[6]	马永金, 刘硕, 李彤, 牛猛, 丁军, 安俐莹. 桥脑中央髓鞘溶解症MRI表现及其在诊断中的应用[J]. 吉林大学学报(医学版), 2015, 41(05): 1041-1043.
[7]	白芃，刘彩霞，贾林沛,刘浩源,苏略,孙维,李幼琼. MRI容量重建技术在颞上沟-侧脑室颞角手术入路选择中的应用[J]. 吉林大学学报(医学版), 2014, 40(06): 1174-1177.
[8]	韩耀启，黄仲奎，龙莉玲，李文美，李理. 兔VX2肝肿瘤多时间点MR增强扫描信号特点及其病理学表现[J]. 吉林大学学报(医学版), 2014, 40(06): 1166-1170.
[9]	王祥，宋军学，张文嘉，赵航，郑杨，李幼琼. 健康人群海马与侧脑室颞角的磁共振线性测量及其意义[J]. 吉林大学学报(医学版), 2014, 40(01): 178-182.
[10]	钟铧,王晓彤,程凯亮,韩青,高倩,王颖,郑雨轩,邵浦,周建英,李幼琼,付强 . 脑固定区域内核磁共振成像测量海马结构体积的方法及结果分析[J]. 吉林大学学报(医学版), 2013, 39(5): 999-1003.
[11]	周广宇，韩雪梅，金玲. 神经精神狼疮患者影像学表现与自身抗体水平和预后的关系[J]. 吉林大学学报(医学版), 2013, 39(3): 609-614.
[12]	周颖，袁博，马越，张琨，高峰，王皓，芦晋，吴晓刚，佟丹，袁长吉 . C6脑胶质瘤模型大鼠MRI影像及形态学观察及其意义[J]. 吉林大学学报(医学版), 2013, 39(2): 379-382.
[13]	舒畅，阚志生，朱雁兵，曹立瀛. 星形细胞瘤MRI定量参数与HIF-1α表达水平的相关性分析[J]. 吉林大学学报(医学版), 2013, 39(1): 109-112.
[14]	沈煜\|沈惠良\|方秀统\|张文博. MRI对骨质疏松性椎体压缩骨折患者椎体终板和骨折椎体邻近间盘损伤的评估及其临床意义[J]. J4, 2012, 38(6): 1205-1208.
[15]	赵继红\|丁军\|徐松柏\|王显荣\|付卫光 . 18F-FDG PET/CT与MRI对乳腺癌诊断价值的比较[J]. J4, 2011, 37(4): 746-749.