高脂血症患者氧化修饰低密度脂蛋白对内皮依赖性血管舒张的影响

doi:吉林省科技厅资助课题(990574-6)

高脂血症患者氧化修饰低密度脂蛋白对内皮依赖性血管舒张的影响

曲极冰¹，包雪鹦²，张明¹，杜红伟^3＊，刘雁军⁴

1. 吉林大学基础医学院药理学教研室，吉林长春130021；2.解放军第208医院，吉林长春 130062；3. 吉林大学第一医院儿科，吉林长春130021；4.美国加利福尼亚州大学洛杉矶分校医学院内分泌研究所，美国洛杉矶90

收稿日期:2005-08-23 修回日期:1900-01-01 出版日期:2006-11-28 发布日期:2006-11-28
通讯作者: 杜红伟

Effect of oxidized low-density lipoprotein on endothelium-dependent relaxation in hyperlipidemic patients

QU Ji-bing¹, BAO Xue-ying² , ZHANG Ming¹, DU Hong-wei^3＊, LIU Yan-jun⁴

1.Department of Pharmacology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China; 2. No. 208 Hospital of PLA, Changchun 130062, China; 3. Department of Pediatrics, First Hospital, Jilin University,Changchun 130021, China; 4.Charles R.Drew University of Medicine and Sciences, Division of Endocrinology, UCLA School of Medicine, Los Angeles 90059, USA

Received:2005-08-23 Revised:1900-01-01 Online:2006-11-28 Published:2006-11-28
Contact: DU Hong-wei

摘要/Abstract

摘要： 目的：探讨氧化修饰低密度脂蛋白（Ox-LDL）对血管舒张的影响及高脂血症男性患者易患动脉粥样硬化（AS）的机制。方法：采用Cu^2＊对13例健康自愿者及29例高脂血症患者的低密度脂蛋白(LDL)进行氧化修饰，硫代巴比妥酸法测定丙二醛（MDA）含量，Bartlett法测定溶血卵磷脂(LPC)含量，乙酰胆碱诱发血管舒张。结果：健康人和高脂血症患者血浆LDL被氧化修饰后与修饰前比较MDA含量显著增高（P<0.01）；两组Ox-LDL中的LPC水平均明显高于各自天然低密度脂蛋白(N-LDL)中的LPC水平（P<0.05）；且健康人及高脂血症男性患者N- LDL中的LPC含量与女性基本相同，但两组男性Ox-LDL中的LPC含量高于女性（P<0.05）,男性高脂血症患者Ox-LDL的LPC含量最高；两组Ox-LDL均明显抑制血管内皮依赖性舒张，与女性相比，健康男性Ox-LDL抑制血管舒张程度略强，但高脂血症男性患者Ox-LDL抑制血管舒张程度明显增强。高脂血症男性及女性患者Ox-LDL的抑制血管舒张效应与LPC量呈正相关（r ＝0.592，P<0.05；r＝0.816，P<0.05)。结论：男性高脂血症患者易患AS可能与Ox-LDL增加LPC含量及抑制内皮依赖性血管舒张有关。

关键词: 血液, 脂蛋白类, LDL, 内皮依赖性血管舒张, 动脉硬化

Abstract: Objective To study the effecet of oxidized low-density lipoprotein(Ox-LDL) on endothelium-dependent relaxation and mechanism of susceptibility to atherosclerosis (AS) in hyperlipdemic male patients. Methods LDL isolated from 13 normal patients and 29 hyperlipidemic patients were modified by CuSO₄. The amount of malondialdehyde (MDA) was measured by TBARS. The amount of lysophosphatidylcholine (LPC) was determined by the Bartlett. Endothelium-dependent relaxation was produced by acetylcholine. Results After LDL from normal and hyperlipidemic patients were modified by CuSO₄, the amount of MDA was increased (P<0.01). LPC in Ox-LDL in two groups were higher than those in their respec tive native LDL(N-LDL). N-LDL contained similar levels of LPC in males and females in two groups, but Ox-LDL from males produced more LPC than that from females in both groups (P<0.05), Ox-LDL from males of hyperlipidemic patients contained the highest level of LPC. Ox-LDL from males and females in both groups inhibited endothelium-dependent relaxation. In hyperlipidemicpatients, Ox-LDL from males caused greater impairment of endothelium-dependent ralaxation than that from females, the effect of gender was minimal within the normal group. Correlation analysis ind icated that the degree of impairment of endothelium-dependent relaxation of male and female hyperlipidemic patients was correlated with the production of LPC during oxidation, (r＝0.592，P<0.05；r＝0.816P<0.05). Conclusion The mechanism of susceptibility to AS in hyperlipdemic male patients is related with the increase of LPC production and greater endothelial dysfunction in Ox-LD L.

Key words: blood, lipoproteins, LDL, endothelium-dependent relaxation, atherosclerosis

中图分类号:

R589.2

曲极冰，包雪鹦，张明，杜红伟，刘雁军. 高脂血症患者氧化修饰低密度脂蛋白对内皮依赖性血管舒张的影响[J]. J4, 2006, 32(6): 1055-1058.

QU Ji-bing, BAO Xue-ying , ZHANG Ming, DU Hong-wei, LIU Yan-jun. Effect of oxidized low-density lipoprotein on endothelium-dependent relaxation in hyperlipidemic patients[J]. J4, 2006, 32(6): 1055-1058.

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