MT蛋奶粉对铅中毒大鼠脑和股骨中微量元素的影响

doi:吉林省省科技厅资助课题（20020503-4）

MT蛋奶粉对铅中毒大鼠脑和股骨中微量元素的影响

胡秀丽¹,荣会²,刘忠英¹，李才^1*

1. 吉林大学再生医学科学研究所分析生化室，吉林长春 130021；2. 吉林出入境检验检疫局，吉林长春 13006

收稿日期:2006-05-15 修回日期:1900-01-01 出版日期:2006-09-28 发布日期:2006-09-28
通讯作者: 李才

Effects of MT egg-milk powder on trace elements in brain and femur of lead poisoning rats

HU Xiu-li¹,RONG Hui²,LIU Zhong-ying¹,LI Cai^1*

1.Department of Analyticl Biochemistry, Institute of Frontier of Medical Sciences,Jilin University,Changchun 130021,China;2.Jilin Entry-Exit Inspection and Quanrantine Burean,Changchun 130062,China

Received:2006-05-15 Revised:1900-01-01 Online:2006-09-28 Published:2006-09-28
Contact: LI Cai

摘要/Abstract

摘要： 目的：探讨含金属硫蛋白（metallothionein，MT）的蛋奶粉对铅中毒大鼠脑和股骨中微量元素铅、铜、锌、铁含量的影响。方法：30只Wistar大鼠随机分为对照组（n=10）、铅中毒组（n=10）和铅中毒MT蛋奶粉处理组（n=10）。大鼠饮服0.2% 醋酸铅水溶液3周造成实验性铅中毒后，灌服500 g•L^-1 MT蛋奶粉3周。实验结束时采集全血、股骨和脑组织，用氰化高铁比色法测定全血血红蛋白含量，用原子吸收分光光度法测定元素铅、铜、锌、铁含量。结果：与对照组比较，铅中毒组大鼠血红蛋白含量明显降低（P<0.05）；股骨和脑中铅、铁含量均显著升高（P<0.01），锌含量明显降低（P<0.05）。铅中毒组大鼠灌服MT蛋奶粉后与铅中毒组比较，血红蛋白含量明显升高（P<0.05），脑、股骨中铅含量均明显降低（P<0.05），脑股骨中铜含量均显著升高（P<0.01）。结论：服用MT蛋奶粉能明显纠正铅中毒大鼠脑和股骨中微量元素代谢紊乱。

关键词: MT蛋奶粉, 铅中毒, 驱铅, 痕量元素

Abstract: Objective To study the effects of MT egg-milk powder on the contents of lead,zinc,iron and copper in brain and femur of lead poisoning rats. Methods 30 Wistar rats were divided into three groups(n=10): normal control group, lead poisoning group and lead poisoning rats treated with MT egg-milk powder group. After treated with 0.2% lead acetate daily for three weeks, the rats were fed with 500 g•L^-1 MT egg-milk powder for three weeks. At the end of the experiment，samples of brain and femur were collected for trace elements detection by atomic absorption spectroscopy. The concentration of hemoglobin was determined by haemiglobincyanide method.Results Compared with the control, the contents of zinc and hemoglobin in brain and femur were significantly decreased (P<0.05), but the contents of lead and iron in brain and femur were significantly elevated（P<0.01）in lead poisoning rats. After the lead poisoning rats were exposed to MT egg-milk powder, the content of lead was decreased (P<0.05),the contents of hemoglobin and copper in both brain and femur were obviously increased（P<0.01）.Conclusion MT egg-milk powder can obviously improve the metabolism disorder of trace elements in lead poisoning rats.

Key words: MT egg-milk power, lead poisoning , excluding lead, trace elements

中图分类号:

R995

胡秀丽,荣会,刘忠英，李才. MT蛋奶粉对铅中毒大鼠脑和股骨中微量元素的影响[J]. J4, 2006, 32(5): 791-793.

HU Xiu-li,RONG Hui,LIU Zhong-ying,LI Cai. Effects of MT egg-milk powder on trace elements in brain and femur of lead poisoning rats[J]. J4, 2006, 32(5): 791-793.

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