基质金属蛋白酶2及其抑制物在人胶质瘤中的表达

doi:吉林省科技厅发展计划基金资助课题

基质金属蛋白酶2及其抑制物在人胶质瘤中的表达

李蕴潜¹，李毅平¹，李才^2*

1. 吉林大学第一医院神经外科，吉林长春 130021；2. 吉林大学再生医学科学研究所病理研究室，吉林长春 130021

收稿日期:2004-05-20 修回日期:1900-01-01 出版日期:2004-11-28 发布日期:2004-11-28
通讯作者: 李才

Expressions of matrix metalloproteinase-2 and its inhibitor in human glioma

LI Yun-qian¹, LI Yi-ping¹，LI Cai^2*

1. Department of Neurosurgery, First Hospital, Jilin University, Changchun 130021,China；2. Department of Pathology, Institute of Frontier Medical Sciences,Jilin University, Changchun 130021, China

Received:2004-05-20 Revised:1900-01-01 Online:2004-11-28 Published:2004-11-28
Contact: LI Cai

摘要/Abstract

摘要： 目的：探讨基质金属蛋白酶2（MMP-2）及其组织抑制物2（TIMP-2）在人胶质瘤的表达特点。方法：按WHO病理分级标准将35例人胶质瘤标本分为4组：Ⅰ级7例，Ⅱ级12例，Ⅲ级10例，Ⅳ级6例。另取4例正常脑组织作为对照。用免疫组化方法检测MMP-2和TIMP-2的表达，分析二者表达强度及阳性染色率与病理分级的关系。结果：MMP-2和TIMP-2阳性染色主要位于胶质瘤细胞浆和细胞外基质。在Ⅰ~Ⅳ级胶质瘤，MMP-2的阳性细胞染色率(%)分别为10.42±3.95、29.92±7.69、45.40±9.01和57.17±11.39，表达强度(分值)分别为2.14±0.38、3.17±0.71、4.00±0.70和5.83±0.41，均随胶质瘤恶性程度的增加而增加；而TIMP-2的细胞阳性染色率(%)分别为51.81±12.28、36.42±9.68、22.10±7.71和4.33±1.63，表达强度(分值)分别为5.57±0.53、3.92±0.29、2.70±0.50和2.17±0.41，均随恶性程度的增加而明显降低。随着胶质瘤恶性程度的增加，MMP-2/TIMP-2比值明显升高。胶质瘤MMP-2和TIMP-2的表达水平之间呈明显负相关（r＝－0.69，P<0.05）。结论：MMP-2和TIMP-2在人胶质瘤的侵袭生长中共同起重要作用。

关键词: 金属蛋白酶2组织抑制剂, 神经胶质瘤, 病理学

Abstract: Objective To investigate the expressions of matrix metalloproteinase-2（MMP-2, gelatinase A）and its inhibitor，tissue inhibitor of metalloproteinase-2（TIMP-2），in human glioma. Methods The samples from 35 cases of glioma were divided into four groups according to the WHO classification: grade Ⅰ (n=7), grade Ⅱ (n=12), grade Ⅲ (n=10)， and grade Ⅳ (n=6), 4 normal brain samples were served as the control. The expressions of MMP-2 and TIMP-2 protein were determined with immunohistochemistry in the samples, and the correlation between the expressions of MMP-2, TIMP-2 and malignant degree of the gliomas was analyzed. Results The positive staining was localized in the cytoplasm of glioma cells and extracellular matrix. In the gliomas from gradeⅠ to gradeⅣ, the positive staining rates (%)(10.42±3.95，29.92±7.69，45.40±9.01， and 57.17±11.39, respectively) and the expression intensities (2.14±0.38，3.17±0.71，4.00±0.70, and 5.83±0.41, respectively) of MMP-2 were significantly elevated with the malignant grade of the gliomas, while the positive staining rates (%)(51.81±12.28，36.42±9.68，22.10±7.71， and 4.33±1.63, respectively) and immunostaining intensities (5.57±0.53，3.92±0.29，2.70±0.50， and 2.17±0.41, respectively) of TIMP-2 were markedly reduced with increasing the grade of glioma malignancy. The ratioes of MMP-2/TIMP-2 were obviously increased when the malignant grades of the gliomas were elevated. There was a negative correlation　(r＝－0.69，P<0.05) between MMP-2 and TIMP-2 expressions in the gliomas. Conclusion MMP-2 and TIMP-2 together play an important role in invasiveness of human glioma.

Key words: tissue inhibitor of metalloproteinase-2, glioma, pathology

中图分类号:

R739.91

李蕴潜，李毅平，李才. 基质金属蛋白酶2及其抑制物在人胶质瘤中的表达[J]. J4, 2004, 30(6): 915-918.

LI Yun-qian, LI Yi-ping，LI Cai. Expressions of matrix metalloproteinase-2 and its inhibitor in human glioma[J]. J4, 2004, 30(6): 915-918.

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