关键词: 人腺样囊性癌细胞, 免疫组织化学

Abstract: To study the inhibitory effect of mistletoe alkali on human adenoid cystic carcinoma (ACC) cells in vitro.0ethods The inhibitory effect on the ACC cells treated by mistletoe alkali was evaluated by CCK-8 assay. The ACC cells treated by 5-FU were used as positive control group and the ACC cells without any treatment as negative control group,meanwhile different doses of mistletoe alkali (0.625,1.250,2.500 and 5.000 mg•L^-1)  were used as experimental groups. The growth of ACC cells after treatment was assessed by cell growth curve,and the cell proliferation was estimated by PCNA immunohistochemistry staining. Results Mistletoe　 alkali inhibited the growth of ACC cells,the value of IC₅₀ was 2.24 mg•L^-1. After treatment,the growth of ACC cells slowed,and the cells became round,and the expression of PCNA was weaker significantly than that in positive control (P<0.001). Conclusion istletoe alkali can inhibit the growth and proliferation of ACC cells obviously in vitro. It might be an ancillary drug to cure ACC.

Key words: adenoid cystic carcinoma cells, immunohistochemistry