羧甲基壳聚糖银对牙龈卟啉单胞菌的抑菌作用

doi:吉林省科技厅科技发展计划重点项目资助课题

羧甲基壳聚糖银对牙龈卟啉单胞菌的抑菌作用

佟巨慧¹, 马琳², 王少鹏³, 迟立超⁴, 付军权⁴, 费瑞⁴

1. 吉林大学口腔医院牙周病科，吉林长春 130041；2. 吉林大学基础医学院第三综合实验室，吉林长春 130021；3.东北师范大学生命科学院生化教研室，吉林长春 130024；4. 吉林大学基础医学院细胞生物学教研室，吉林

收稿日期:2007-12-13 修回日期:1900-01-01 出版日期:2008-07-28 发布日期:2008-07-28
通讯作者: 费瑞

Antimicrobial effect of silver carboxymethyl chitosan against porphyromonas gingivalis

TONG Ju-hui¹, MA Lin², WANG Shao-peng³, CHI Li-chao⁴, FU Jun-quan⁴, FEI Rui⁴

1. Department of Periodontal Disease, Stomatology Hospital, Jilin University, Changchun 130041, China; 2. Third Combined Laboratory, School of Basic Medical Sciences, Jilin University, Changchun 130021,China; 3. Department of Biochemistry, School of Life Science, Northeast Normal University, Changchun 130024, China; 4. Department of Cell Biology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China

Received:2007-12-13 Revised:1900-01-01 Online:2008-07-28 Published:2008-07-28
Contact: FEI Rui

摘要/Abstract

摘要： 目的：研究羧甲基壳聚糖银 (CMCT-Ag⁺) 对牙龈卟啉单胞菌 (Pg) 的抑制作用，为研制高效无毒的抗牙周病药物提供实验依据。方法：将壳聚糖经碱化、氯乙酸修饰和离子交换制成 CMCT-Ag⁺。采用琼脂扩散和固体平板二倍稀释法观察抑菌环直径和菌斑数，测定 CMCT-Ag⁺ 对 Pg的抑制作用。结果：获得了粉红色的 CMCT-Ag⁺ 粉末，收率为 89.4%，取代度为 0.98，银离子含量为10.21%。在抑菌环实验中，当CMCT-Ag⁺的浓度大于 0.3125 g•L^-1 时，其抑菌环直径随浓度的增加而增大，其中 40 g•L^-1CMCT-Ag⁺ 抑菌环直径可达 9.5 mm，与 0.005 g•L^-1 替硝唑相当；当 CMCT-Ag⁺ 浓度大于 40 g•L-1 时，抑菌环直径明显下降，表明 CMCT-Ag⁺ 的抑菌范围为大于 0.3125 g•L^-1。在菌斑实验中，当 CMCT-Ag⁺ 浓度大于 0.3125 g•L^-1 时，培养基表面 Pg 的生长明显受到抑制；而当浓度大于 1.25 g•L^-1 时，培养基表面无 Pg 生长，表明 CMCT-Ag⁺ 最低抑菌浓度为1.25 g•L^-1。结论：CMCT-Ag⁺ 对 Pg 的生长有明显抑制作用，可用于进一步研制治疗牙周炎药物。

关键词: 牙龈卟啉单胞菌, 牙周炎

Abstract: Objective To investigate the antimicrobial effect of silver carboxymethyl chitosan (CMCT-Ag⁺) on porphyromonas gingivalis (Pg), a critical bacteria in periodontitis and provide experimental basis for developing higher efficiency and lower side-effect drug to cure periodontitis. Methods CMCT was alkalified, modified with chloracetic acid and exchanged ion to obtain CMCT-Ag⁺ complexation. The agar diffusion and plate serial dilution test was used to detect the effect of CMCT-Ag⁺ against Pg by observing cingula diameter and bacteria plaque. Results The pink CMCT-Ag⁺ was obtained, yield ratio was 89.4 %, the degree of substitution of CMCT was 0.98 and the content of Ag⁺ was 10.21% in the obtained CMCT-Ag⁺. In the agar diffusion test, when concentration of CMCT-Ag⁺ exceed 0.3125 g•L^-1, the bacteriostasis ring diameter was increased, and at concen tration of 40 g•L^-1, the bacteriostasis ring diameter inhibited by CMCT-Ag⁺ was 9.5 mm，that was approximation to the result induced by 0.005 g•L^-1 tinidazole. But when concentration of CMCT-Ag⁺ exceed 40 g•L^-1, the bacteriostasis ring diameter was decreased. These results showed that the inhibitory concentration of CMCT-Ag⁺ was more than 0.3125 g•L^-1. In the plate serial dilution test, when concentration of CMCT-Ag⁺ exceed 0.3125 g•L^-1, the growth of Pg was obviously inhibited on medium, and when concentration of CMCT-Ag⁺ exceed 1.25 g•L^-1, the growth of Pg couldn’[KG-*3]t be found. These results indicated that the minimal inhibitory concentration (MIC) of CMCT-Ag⁺ was 1.25 g•L^-1. Conclusion CMCT-Ag⁺ could inhibit the growth of Pg, hence it can be used for periodontal treatment.

Key words: porphyromonas gingivalis, periodontitis

中图分类号:

R988.2

佟巨慧, 马琳, 王少鹏, 迟立超, 付军权, 费瑞. 羧甲基壳聚糖银对牙龈卟啉单胞菌的抑菌作用[J]. J4, 2008, 34(4): 636-639.

TONG Ju-hui, MA Lin, WANG Shao-peng, CHI Li-chao, FU Jun-quan, FEI Rui. Antimicrobial effect of silver carboxymethyl chitosan against porphyromonas gingivalis[J]. J4, 2008, 34(4): 636-639.

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