吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (04): 681-684.doi: 10.13481/j.1671-587x.20160409

• 基础研究 • 上一篇    下一篇

MT01对牙龈卟啉单胞菌感染成骨细胞MG63中Ⅰ型胶原mRNA表达的促进作用及其意义

刘引1,2, 申玉芹1, 高涵1,2, 费鸿博1,2, 胡天琦1,2, 李洋洋1,2, 顾中一1,2, 林崇韬1   

  1. 1. 吉林大学口腔医院牙周病科, 吉林 长春 130021;
    2. 吉林大学口腔医院吉林省牙发育及颌骨重塑与再生重点实验室, 吉林 长春 130021
  • 收稿日期:2015-12-25 发布日期:2016-07-20
  • 通讯作者: 林崇韬,教授,硕士研究生导师(Tel:0431-88796039,E-mail:linct@jlu.edu.cn) E-mail:linct@jlu.edu.cn
  • 作者简介:刘引(1990-),女,安徽省宿州市人,在读医学硕士,主要从事牙周病病因及组织再生机制方面的研究。
  • 基金资助:

    国家自然科学基金面上项目资助课题(81371153);吉林省科技厅自然科学基金面上项目资助课题(20150101173JC)

Effects of MT01 on expression of collagen Ⅰ mRNA in osteoblasts MG63 infected by Porphyromonas gingivalis and its significance

LIU Yin1,2, SHEN Yuqin1, GAO Han1,2, FEI Hongbo1,2, HU Tianqi1,2, LI Yangyang1,2, GU Zhongyi1,2, LIN Chongtao1   

  1. 1. Department of Periodontology, Stomatology Hospital, Jilin University, Changchun 130021, China;
    2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Jilin University, Changchun 130021, China
  • Received:2015-12-25 Published:2016-07-20

摘要:

目的:检测单链寡脱氧核苷酸MT01 作用下牙龈卟啉单胞菌(Pg) 感染成骨细胞MG63中Ⅰ型胶原(ColⅠ)mRNA 表达水平,探讨MT01对感染状态下成骨细胞成骨向分化的影响。方法:体外培养的MG63细胞分为空白对照组、MT01组、Pg组和MT01+Pg组。按分组情况进行相应处理,加入MT01(质量浓度1 mg·L-1),以1 mg·L-1PBS作对照共孵育3h后,以100∶1的感染复数(MOI)加入Pg悬液共培养。4组细胞分别孵育2、4、6、8、12和24h后采用RT-PCR法检测MG63细胞中ColⅠ mRNA表达水平。结果:与空白对照组比较,MT01和MT01+Pg组MG63细胞中ColⅠ mRNA表达水平在2和4h时无明显变化(P > 0.05),8、12和24h时MT01组细胞中ColⅠ mRNA表达水平明显升高(P < 0.05或P < 0.01);与Pg组比较,2和4h时MT01+Pg组细胞中ColⅠ mRNA呈低表达,6、8、12和24h时ColⅠ mRNA表达水平升高(P < 0.05或P < 0.01)。结论:MT01上调感染状态下MG63细胞中ColⅠ mRNA的表达水平,MT01可促进感染状态下成骨细胞成骨向分化。

关键词: 单链寡脱氧核苷酸MT01, 牙龈卟啉单胞菌, 人成骨细胞, Ⅰ型胶原

Abstract:

Objective: To investigate the effect of MT01 on the differentiation of osteoblasts under infected condition through determining the expression level of collagen Ⅰ(ColⅠ) mRNA in MG63 cells treated with Porphyromonas gingivalis(Pg). Methods: The cultured MG63 cells were divided into blank control,MT01,Pg, and MT01+Pg groups.MT01 at a concentration of 1 mg·L-1 was added into the MG63 cells,and the cells were incubated for 3 h.The cells treated with PBS (1 mg·L-1) were used as control group.Then Pg(MOI=100∶1)was added.Real-time PCR was used to detect the expression levels of ColⅠ mRNA in MG63 cells at 2,4,6,8,12 and 24 h after incubation. Results: Compared with blank control group,the levles of ColⅠ mRNA in the MG63 cells in MT01 and MT01+Pg groups had no significant changes at 2 and 4 h(P > 0.05);the ColⅠ mRNA expression levels in MT01 group at 8,12 and 24 h were increased(P < 0.05 or P < 0.01).Compared with Pg group, the expression levels of ColⅠ mRNA in MT01+ Pg at 2 and 4 h were decreased,but the expression levels of ColⅠ mRNA were increased at 6,8,12 and 24 h(P < 0.05 or P < 0.01). Conclusion: MT01 can up-regulate the expression level of ColⅠ mRNA in the infected MG63 cells;MT01 could promot the differentiation of osteoblasts under infected condition.

Key words: specific sequence oligodeoxynucleotide MT01, Porphyromonas gingivalis, human osteoblast cell, collagen Ⅰ

中图分类号: 

  • Q254