关键词: 药理学, 癌, Lewis肺, 毛细血管, 病理学, 内皮生长因子, 生物合成

Abstract: Objective To explore the inhibitory effects of topotecan on mice with Lewis lung cancer(LLC) and its mechanisms. Methods LLC cells were inoculated subcutaneously into C57BL/6 mice. Fifty-five mice were randomly divided into control group (n=10) and experimental groups (Topotecan of 0.312,0.624， and 1.248 mg•kg^-1,15 in each group).Topotecan and phosphate- buffered saline vehicle were injected intraperitoneally, respectively.On the 21th day ,the mice were killed. Tumor growth curve was described, the inhibitory rate of primary tumor was measured. Microvessel density (MVD) was quantitated by immunohistochemistry using monoclonal antibodies against factor F8, vascular endothelial growth factor (VEGF) immunoreactivity was examined by immunohistochemistry using polyclonal antibodies against VEFG. Results Tumor growth curves in experimental groups were smooth compared with control group.There were significant differences of inhibitory rates of primary tumor between control and experimental groups(P<0.05);there were significant differences of MVD between high,middle dosages of topotecan groups and control (P<0.05).VEGF-positive rates were not significantly different between control and experimental groups. Conclusion Topotecan can effectively suppress the growth of the implanted LLC in C57BL/6 mouse and can inhibit angiogenesis.

Key words: pharmacology, carcinoma, Lewis lung, capillaries, pathology, endothelial growth factors, biosynthesis