吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (05): 862-866.doi: 10.13481/j.1671-587x.20170502

• 基础研究 • 上一篇    下一篇

白细胞介素37对宫颈癌HeLa细胞顺铂化疗敏感性的增强作用

邓子亮, 王森, 黎鹏, 解奎龙, 李淑贤, 周世雄, 贺晓舟, 陈东, 郭洪胜   

  1. 广东医科大学基础医学院组织学与胚胎学教研室, 广东 东莞 523808
  • 收稿日期:2017-03-09 出版日期:2017-09-28 发布日期:2017-09-29
  • 通讯作者: 郭洪胜,副教授(Tel:0769-22896382,E-mail:6806003@163.com) E-mail:6806003@163.com
  • 作者简介:邓子亮(1987-),男,安徽省阜阳市人,助理实验师,医学硕士,主要从事肿瘤和细胞免疫方面的研究。
  • 基金资助:
    国家自然科学基金资助课题(81302244);广东省科技厅科技发展计划项目资助课题(2016A020215147,2013B021800062);广东省卫计委医学科研基金项目资助课题(A2016208,B2013293);广东省东莞市科技局社会发展重点基金项目资助课题(2013108101051);广东医科大学科研基金资助课题(M2016028);广东省湛江市科技局科技计划项目资助课题(2013B01092)

Enhancement of IL-37 in chemosensitivity of cervical cancer HeLa cells to cisplatin

DENG Ziliang, WANG Sen, LI Peng, XIE Kuilong, LI Shuxian, ZHOU Shixiong, HE Xiaozhou, CHEN Dong, GUO Hongsheng   

  1. Department of Histology and Embryology, College of Basic Medical Sciences, Guangdong Medical University, Dongguan 523808, China
  • Received:2017-03-09 Online:2017-09-28 Published:2017-09-29

摘要: 目的:通过将白细胞介素37(IL-37)基因转染到宫颈癌HeLa细胞中,探讨IL-37对宫颈癌细胞的杀伤作用及其对宫颈癌细胞化疗敏感性的增强作用。方法:将重组pIRES2-EGFP/IL-37质粒(IL-37组)和pIRES2-EGFP质粒(NC组)分别转染到HeLa细胞。Q-PCR和Western blotting法检测IL-37基因和蛋白表达水平;CCK8法检测NC组、IL-37组、顺铂(DDP)组及IL-37+DDP组细胞活性,计算细胞抑制率;RT-PCR法检测信号转导和转录激活因子3(STAT3)及细胞周期蛋白D1(Cyclin D1) mRNA表达水平。结果:与NC组比较,IL-37组IL-37 mRNA和蛋白表达水平明显升高(P < 0.01)。给药后24~72 h,5~15 mg·L-1 DDP组HeLa细胞活性受到明显抑制(P < 0.01);与DDP组比较,IL-37+DDP组HeLa细胞抑制率在处理后48 h内明显升高(P < 0.05);与IL-37组比较,IL-37+DDP组HeLa细胞抑制率在处理后96 h内均有升高(P < 0.05)。与NC组比较,IL-37组STAT3和Cyclin D1 mRNA表达水平明显下降(P < 0.01)。结论:IL-37高表达可抑制宫颈癌细胞的增殖,并能增强DDP对宫颈癌细胞的放疗效果,这种效应的发挥可能与IL-37使STAT3和Cyclin D1表达下调有关。

关键词: 宫颈肿瘤, 顺铂, 化疗敏感性, 白细胞介素37

Abstract: Objective: To transfer the interleukin 37 (IL-37)gene to cervical cancer Hela cells, and to explore the killing effect of IL-37 on the HeLa cells and its enhancement in the chemotherapy sensitivity of HeLa cells. Methods: The pIRES2-EGFP (NC group) and pIRES2-EGFP/IL-37(IL-37 group) plasmids were transfected into the HeLa cells. Q-PCR and Western blotting methods were used to detect the expression levels of IL-37 mRNA and protein. The activities of HeLa cells in NC group, IL-37 group, DDP group and IL-37+DDP group were detected by CCK8 method,and the inhibitory rates of cells were calculated. The gene expressions of signal transducer and activator of transcription 3 (STAT3) and Cyclin D1 were detected by RT-PCR method. Results: Compared with NC group, the expression levels of IL-37 mRNA and protein in IL-37 group were significantly increased(P<0.01). The activities of HeLa cells in DDP(5-15 mg·L-1) groups were inhibited after administration for 24-72 h (P<0.01);the inhibitory rates in IL-37+DDP group were higher than those in DDP group within 48 h after administration (P<0.05).Compared with IL-37 group,the inhibitory rates in IL-37+DDP group was increased with 96 h after administration (P<0.05).Compared with NC group, the expression levels of STAT3 and Cyclin D1 mRNA in IL-37 group were significantly decreased (P<0.01). Conclusion: The over-expression of IL-37 can inhibit the proliferation of cervical cancer cells and enhance the effect of DDP on the chemotherapy of cervical cancer cells which may be related to the down-regulation of the expressions of STAT3 and Cyclin D1 by IL-37.

Key words: cervical neoplasms, cisplatin, chemotherapy sensitivity, interleukin 37

中图分类号: 

  • R737.33