特发性肺纤维化患者外周血单个核细胞T淋巴细胞亚群及Th1/Th2型细胞因子的检测

doi:国家自然科学基金资助课题（30370617）;吉

特发性肺纤维化患者外周血单个核细胞T淋巴细胞亚群及Th1/Th2型细胞因子的检测

吴春风^1,2，马忠森¹，王秀丽³，杨广民⁴，马寅芙⁴，杨俊玲¹，尹金植¹，陈鹏¹

1．吉林大学第二医院呼吸内科，吉林长春 130041；2．吉林省人民医院呼吸疗区，吉林长春 130021；3．吉林大学第二医院血液科，吉林长春 130041；4．吉林省人民医院中心实验室，吉林长春 130021

收稿日期:2007-09-06 修回日期:1900-01-01 出版日期:2008-03-28 发布日期:2008-03-28
通讯作者: 马忠森

Determination of T lymphocytes subset and cytokine of Th1/Th2 lymphocytes in peripheral blood mononuclear cells from patients with　idiopathic pulmonary fibrosis

WU Chun-feng^1,2，MA Zhong-sen¹，WANG Xiu-li³，YANG Guang-min⁴，MA Yin-fu⁴，YANG Jun-ling¹，YIN Jin-zhi¹，CHEN Peng¹

1.Department of Respiratory Diseases, Second Hospital, Jilin University, Changchun 130041, China; 2.Department of Respiratory Diseases,Peoples’ Hospital of Jilin Province, Changchun 130021, China; 3.Department of Hematology, Second Hospital, Jilin University, Changchun 130041, China; 4.Central Laboratory,Peoples’ Hospital of Jilin Province, Changchun 130021, China

Received:2007-09-06 Revised:1900-01-01 Online:2008-03-28 Published:2008-03-28
Contact: MA Zhong-sen

摘要/Abstract

摘要： 目的：通过流式细胞术检测特发性肺纤维化(IPF)患者外周血T淋巴细胞亚群及Th1/Th2型细胞因子水平，探讨其在IPF发病机制中的作用及临床意义。方法：采用流式细胞术检测25例IPF患者外周血单个核细胞(PBMC)的T淋巴细胞亚群(CD3⁺、CD4⁺及CD8⁺)以及Th1型细胞因子(IFN-γ)和Th2型细胞因子(IL-4)水平，并观察CD4⁺/CD8⁺和Th1/Th2比值变化。25例健康体检者为正常对照组。结果：IPF组患者CD4⁺以及CD4⁺/CD8⁺比值均低于正常对照组(P<0.05)，CD8⁺明显高于正常对照组 (P<0.01)，CD3⁺水平与正常对照组比较差异无显著性(P>0.05)。IPF组患者IL-4水平高于正常对照组(P<0.01)，IFN-γ的水平与正常对照组比较差异无显著性(P>0.05)，Th1/Th2比值显著低于正常对照组(P<0.01)。结论：细胞免疫过强和Th2型免疫优势可能参与IPF发病，这将为IPF的诊断和细胞因子治疗提供理论基础。

关键词: Th1, Th2, T淋巴细胞亚群, 流式细胞术

Abstract: Objective To detect T lymphocytes subset and Th1/Th2 of peripheral blood from patients with idiopathic pulmonary fibrosis(IPF) by flow cytometry (FCM) and evaluate the role of them in pathogenesis and clinical significance. Methods The levels of T lymphocyte subset (CD3⁺,CD4⁺, CD8⁺), IFN-γ belonged to Th1 and IL-4 belonged to Th2 in peripheral blood mononuclear cells(PBMC)were detected by FCM and the ratio of CD4⁺/CD8⁺ ,Th1/Th2 were analyzed from 25 patients with IPF. 25 healthy volunteers were used as controls. Results The level of CD4⁺ and the ratio of CD4⁺/ CD8⁺ in IPF group were lower than those in control group(P<0.05). The level of CD8⁺ in IPF group was obviously higher than that in control group(P<0.01),while CD3⁺ had no change (P>0.05). The level of IL-4 in IPF group was significantly higher than that in control group(P<0.01),while the level of IFN-γ had no change(P>0.05).The ratio of Th1/Th2 in IPF group was significantly lower than that in control group (P<0.01). Conclusion The excessive cell immunity and immunodominance of Th2 with disbalance of Th1/Th2 may participate in pathogenesis of IPF,which will supply rationale foundation for diagnosis and cytokine therapy of IPF.

Key words: Th1, Th2, T lymphocyte subset, flow cytometry

中图分类号:

R563.1

吴春风，马忠森，王秀丽，杨广民，马寅芙，杨俊玲，尹金植，陈鹏. 特发性肺纤维化患者外周血单个核细胞T淋巴细胞亚群及Th1/Th2型细胞因子的检测[J]. J4, 2008, 34(2): 282-284.

WU Chun-feng，MA Zhong-sen，WANG Xiu-li，YANG Guang-min，MA Yin-fu，YANG Jun-ling，YIN Jin-zhi，CHEN Peng. Determination of T lymphocytes subset and cytokine of Th1/Th2 lymphocytes in peripheral blood mononuclear cells from patients with　idiopathic pulmonary fibrosis[J]. J4, 2008, 34(2): 282-284.

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