关键词: 乙型, 慢性, CD4^+<, sup>CD25^+<, sup>调节性T细胞, CD127

Abstract: Abstract:Objective To investigate the proportion of CD4⁺CD25^highTreg and CD4⁺CD25⁺CD127^low/-Treg in peripheral blood in patients with chronic hepatitis B (CHB) and determine the relationship between the proportion of CD4⁺CD25⁺Treg and clinical parameters. Methods Fresh isolated peripheral blood mononuclear cells (PBMCs) of 28 patients with CHB and 24 healthy donors were analyzed for the proportion of CD4⁺CD25⁺Treg using flow cytometry by surface staining for CD4-PC5，CD25-FITC，CD127-PE. HBsAg,HBsAb,HBeAg,HBeAb and HBcAb were evaluated.HBV DNA levels were measured using real-time RT-PCR. Results The proportions of CD4⁺CD25^highTreg to CD4⁺ T cells (3.36%±2.59%) and CD4⁺CD25⁺CD127^low/-Treg (4.05%±1.63%) to CD4⁺ T cells in patients with CHB were higher than those inhealth controls(1.60%±0.66%, 1.75%±0.83%, P<0.01) in peripheral blood. The CHB patients with high level alanine amino transferase (ALT) (ALT>100U•L^-1) had a higher fraction (4.26%±3.10%) of CD4⁺CD25^highTreg in peripheral blood than those patients with low level ALT (ALT＜100U•L^-1,2.52%±2.72%,P<0.05). The percentage of CD4⁺CD25^highTreg was correlated with ALT level (r=0.44，P<0.05). Conclusion The level of Treg in CHB patients is higher than that in health controls. The expression of CD25 could be influenced by the level of ALT. As a specific cell surface marker, CD127 can be helpful to obtain pure CD4⁺CD25⁺Treg.

Key words: JIANG Yan-fang, LI Wan-yu, NIU Jun-qi