降脂合剂对高脂血症大鼠血脂及血流异常的调节和抑制作用

J4 ›› 2012, Vol. 38 ›› Issue (5): 937-942.

降脂合剂对高脂血症大鼠血脂及血流异常的调节和抑制作用

谢慧臣¹|刘芬¹|杨强²

1．湖北民族学院医学院中西医结合教研室|湖北恩施 |445000；2．湖北民族学院医学院附属医院中西医结合科|湖北恩施 |445000

收稿日期:2012-05-08 出版日期:2012-09-28 发布日期:2012-09-28
通讯作者: 刘芬(Tel:0718-8437479，E-mail:liuflower813@hotmail.com) E-mail:liuflower813@hotmail.com
作者简介:谢慧臣（1975-）|男|湖北省恩施市人|副教授|医学博士|主要从事经方防治心血管系统疾病的临床与基础研究。
基金资助:
湖北省教育厅科学研究计划项目资助课题(B20092905);湖北民族学院博士科研启动基金资助课题（20100213）

Regulation and inhibition of lipid-lowering mixture on blood lipid and blood flow abnormalities of hyperlipemia rats

XIE Hui-chen¹,LIU Fen¹,YANG Qiang²

1. Department of Integrated Traditional Chinese and Western Medicine,School of Medical Science,Hubei Nationalities College, Enshi 445000,China； 2. Department of Integrated Chinese and Western Medicine, Affiliated Hospital,School of Medical Science,Hubei Nationalities College, Enshi |445000,China

Received:2012-05-08 Online:2012-09-28 Published:2012-09-28

摘要/Abstract

摘要：

目的:探讨降脂合剂对高脂血症大鼠血脂谱及血液流变学的影响，阐明降脂合剂调节血脂及血流动力学的作用机制。方法：将雄性Wistar大鼠60只随机分为正常对照组、模型对照组、辛伐他汀组和降脂合剂高、中、低剂量组，每组10只。除正常对照组用基础饲料喂养外，其余各组大鼠喂饲高脂饲料建立高脂血症大鼠模型，造模同时预防给药，空白对照组及模型对照组大鼠给予生理盐水2 mL灌胃，辛伐他汀组大鼠给予7.2×10-4g/mL的辛伐他汀混悬溶液2 mL灌胃，降脂合剂低、中、高剂量组大鼠分别给予0.25、0.50、1.00 g/mL的中药煎剂2 mL灌胃，以上给药均为每日1次，持续喂养及给药10周。10周后6组大鼠均摘眼球取血，全自动生化分析仪测定大鼠血脂谱，全自动血液流变分析仪测定大鼠血液流变学的变化。结果：与模型组比较，降脂合剂各剂量组大鼠预防用药后，胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL-C）和载脂蛋白B(ApoB)的水平均不同程度降低，高密度脂蛋白（HDL-C）、载脂蛋白A（ApoA）的水平及apoA／apoB比值均有不同程度的升高，差异均有统计学意义（P<0.05或P<0.01）；与模型组比较，降脂合剂各组大鼠血浆黏度（ηp）、全血黏度（ηb）（低、中、高切）、红细胞聚集指数（IEA）、红细胞压积(HCT)及红细胞电泳时间（EET）降低，红细胞变形指数（IED）升高，差异均有统计学意义（P<0.05或P<0.01）。结论：降脂合剂能够调节实验性高脂血症大鼠血脂代谢及血液的黏、聚、凝状态，对血脂和血流异常有较好的调节和抑制作用。

关键词: 降脂合剂；高脂血症；血脂谱；载脂蛋白；血液流变

Abstract:

Abstract:Objective To study the effect of Lipid-lowering Mixture(LM) on blood lipid profile and blood hemorheology of hyperlipidemia rats，and to elucidate the mechanism of LM on regulating blood lipid and hemorhelogy. Methods 60 male Wistar rats were randomly divided into normal control group, model control group,simvastatin group and LM high,middle, low doses groups,and there were 10 rats in each group.The rats in normal control group were fed with normal diet,and the rats in the other groups were fed with a high fat diet to establish hyperlipidemia rat models, and during the modeling the drugs were used for prevention.2 mL physiological saline were given to the rats in control group and model group,and the rats in simvastatin group were given 7.2×10-4g/mLsimvastatin suspension solution 2 mL, and the rats in LM low, middle,high doses groups were given 0.25,0.50,1.00 g/mL decoction 2 mL,the once daily dose lasted for 10 weeks.After 10 weeks,the rats in 6 groups were eyeballed blood, the blood lipid profile and blood hemorheology of each animal in various groups were measured by full automatic biochemical analyzer and full automatic blood analyzer,respectively.Results Compared with model group, after treatment, the levels of the cholesterol (TC ), triglyceride ( TG ),low density lipoprotein ( LDL-C ) and apolipoprotein B ( ApoB ) in LM groups were reduced; the high density lipoprotein ( HDL-C ), apolipoprotein A ( ApoA ) levels and apoA / apoB ratio were increased, the differences were statistically significant ( P<0.05 or P<0.01).Compared with model group, the plasma viscosity（ηp）, whole blood viscosity ( low, medium, high cut ) （ηb）, index of erythrocyte aggregation ( IEA ), hematocrit (HCT ),and erythrocyte electrophoresis time ( EET ) in LM groups were decreased, the index of erythrocyte deformability ( IED ) was increased, the differences were statistically significant ( P<0.05 or P<0.01).Conclusion LM can modulate blood lipid metabolism and blood viscosity, poly, and prothrombotic state of the experimental hyperlipidemia rats, which has better regulation and inhibition on blood lipid and blood flow abnormalities.

Key words: lipid-lowering mixture, hyperlipidemia, lipid profile, apolipoprotein, blood rheology

中图分类号:

R285.5

谢慧臣|刘芬|杨强. 降脂合剂对高脂血症大鼠血脂及血流异常的调节和抑制作用[J]. J4, 2012, 38(5): 937-942.

XIE Hui-chen,LIU Fen,YANG Qiang. Regulation and inhibition of lipid-lowering mixture on blood lipid and blood flow abnormalities of hyperlipemia rats[J]. J4, 2012, 38(5): 937-942.

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