重组人酸性成纤维细胞生长因子脂质体的制备及其物理稳定性评价

J4 ›› 2012, Vol. 38 ›› Issue (5): 932-936.

重组人酸性成纤维细胞生长因子脂质体的制备及其物理稳定性评价

滕跃^1|2|田海山^1|3|张睿¹|焦悦¹|李校堃^1|3

1.吉林农业大学　生物反应器与药物开发教育部工程研究中心|吉林长春 130118；2.吉林农业大学生命科学学院|吉林长春 130118；3.温州医学院|浙江温州 325035

收稿日期:2012-04-24 出版日期:2012-09-28 发布日期:2012-09-28
通讯作者: 田海山（Tel: 0577-86689983,E-mail：tianhaishan332@sina.com）；李校堃(Tel: 0431-84533348,E-mail：xiaokunli@163.net) E-mail:tianhaishan332@sina.com；xiaokunli@163.net
作者简介:滕跃(1985-)|女|内蒙古自治区突泉县人|在读生物化学与分子生物学硕士|主要从事基因工程制药的研究。
基金资助:
吉林省教育厅科研基金资助课题［2009(65)］

Preparation of rhaFGF liposomes and evaluation of their physical stability 

TENG Yue^1|2,TIAN Hai-shan^1|3,ZHANG Rui¹,JIAO Yue¹,LI Xiao-kun^1|3

1.Engineering Research Center of Bioreactor and Pharmaceutical Development,Ministry of Education,Jilin Agricultural University,Changchun 130118,China|2.College of Life Science,Jilin Agricultural University,Changchun 130118,China|3.Wenzhou Medical College,Wenzhou 325035,China

Received:2012-04-24 Online:2012-09-28 Published:2012-09-28

摘要/Abstract

摘要：

［摘要］目的：确立重组人酸性成纤维细胞生长因子（rhaFGF）脂质体制备的最佳处方及工艺条件，研究其理化性质。方法：采用冻干再水化法，以磷脂与胆固醇质量之比、缓冲溶液pH值和均质时间为单因素，每个因素选取三水平进行实验，以药物包封率、物理稳定性和生物活性作为评价指标，确立优化脂质体制备的最佳处方及工艺条件。结果：采用最佳处方工艺条件，即磷脂与胆固醇的比例为20∶1，磷酸盐缓冲液pH值为6.5，高压均质时间为20 min制备的rhaFGF脂质体，经测定其包封率（En）达81.79%±2.51%，物理稳定性参数（KE）为1.050%±0.342%，生物学活性为（6.521 0±0.617 1)×10⁵Ｕ/mL，粒子大小较均匀，粒径大多分布在100 nm左右。结论：通过优化工艺条件制备的rhaFGF脂质体包封率高，在4℃保存条件下，渗漏率低且具有很好的物理稳定性及生物学活性。

关键词: 重组人酸性成纤维细胞生长因子；脂质体；包封率；渗漏率

Abstract:

Abstract:Objective To optimize the prescription and preparation of recombinant human acidic fibroblast growth factor(rhaFGF) liposomes,and to study the physical and chemical properties. Methods The encapsulation efficiency and biological activity of rhaFGF liposomes were evaluated by single factor test. Freeze-drying-rehydration was used to obtain the optimum prescription and preparation of rhaFGF liposomes. Results The encapsulation efficiency of rhaFGF liposomes was (81.79±2.51)%,the physical stability index (KE) was (1.050±0.342)% and the bioactivity was (6.521 0±0.617 1)×105U/mL. The particle size was about 100 nm uniformly. Conclusion The preparation of rhaFGF liposomes is optimized,and these liposomes have high encapsulation efficiency,good physical stability and high bioactivity at 4℃.

Key words: recombinant human acidic fibroblast growth factor, liposome, encapsulation efficiency, percolation ratio

中图分类号:

滕跃|田海山|张睿|焦悦|李校堃. 重组人酸性成纤维细胞生长因子脂质体的制备及其物理稳定性评价[J]. J4, 2012, 38(5): 932-936.

TENG Yue,TIAN Hai-shan,ZHANG Rui,JIAO Yue,LI Xiao-kun. Preparation of rhaFGF liposomes and evaluation of their physical stability [J]. J4, 2012, 38(5): 932-936.