吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (2): 227-231.doi: 10.7694/jldxyxb20130208

• 基础研究 • 上一篇    下一篇

巨核细胞蛋白质酪氨酸磷酸酶2的克隆表达及其与骨质疏松症的关系

张蕾1,张师桃1,曾晛阳1,张小平2,郝凡凡2,王丰1,李婉南1,付学奇1   

  1. (1.吉林大学生命科学学院Fischer细胞信号转导实验室,吉林 长春 130012;2.吉林大学中日联谊医院妇产科,吉林 长春 130033)
  • 收稿日期:2012-12-27 出版日期:2013-03-28 发布日期:2013-03-28
  • 通讯作者: 李婉南 E-mail:(Tel:0431-85155321,E-mail: liwannan@jlu.edu.cn)
  • 作者简介:张 蕾(1984-),女,黑龙江省牡丹江市人,在读分子生物学博士,主要从事微生物与生化药学方面的研究。
  • 基金资助:

    国家自然科学基金青年基金资助课题(81102859)

Cloning and expression of megakaryocyte protein tyrosine phosphatase 2  and its relationship with osteoporosis


ZHANG Lei1,ZHANG Shi-tao1,ZENG Xian-yang1,ZHANG Xiao-ping2,HAO Fan-fan2,WANG Feng1,LI Wan-nan1,FU Xue-qi1   

  1. (1.Edmond H.Fischer Signal Transduction Laboratory,College of Life Sciences,Jilin University,Changchun  130012,China;2. Department of Gynecology and Obstetrics,China-Japan Union Hospital,Jilin University,Changchun 130033,China)
  • Received:2012-12-27 Online:2013-03-28 Published:2013-03-28

摘要: 目的:探讨巨核细胞蛋白质酪氨酸磷酸酶2(PTPMEG2)的克隆表达及其对骨质疏松症发展过程的调节作用。方法:分离纯化后的PTPMEG2蛋白,应用免疫学技术制备PTPMEG2的多克隆抗体。30只雌性SD大鼠随机分为模型组(n=15)和对照组(n=15),采用维甲酸灌胃给药的方法构建大鼠骨质疏松症模型,对照组大鼠采用生理盐水灌胃,检测2组大鼠血清中酸性磷酸酶(ACP)及碱性磷酸酶(ALP)的水平。Western blotting法检测大鼠各组织中PTPMEG2的表达水平,并对比PTPMEG2在骨髓细胞(BMCs)、红细胞及骨髓基质细胞(BMSCs)中表达量的变化。结果:成功制备PTPMEG2的多克隆抗体。模型组大鼠血清中ACP和ALP表达水平均高于对照组(P<0.01),且模型组大鼠的骨脆性明显高于对照组。模型组大鼠肾脏组织中PTPMEG2的表达水平高于对照组(P<0.05),而其在肌肉组织中的表达水平却低于对照组(P<0.05)。与对照组比较,PTPMEG2在模型组大鼠BMCs中表达量显著增加,而在红细胞和BMSCs中其表达量无明显差异。结论:骨质疏松症大鼠BMCs中PTPMEG2表达水平增加,且多发生在骨髓内分化成熟的骨细胞中; PTPMEG2可能在骨质疏松症的发展过程中发挥重要的调节作用。

关键词: 巨核细胞蛋白质酪氨酸磷酸酶2, 多克隆抗体, 骨质疏松症

Abstract: To research the cloning and expression of the megakaryocyte protein tyrosine phosphatase 2(PTPMEG2) and its regulatory role in osteoporosis development.Methods
The purified PTPMEG2 was used to prepare PTPMEG2 polyclonal antibody.30 female SD rats were rardomly divded into model group(n=15) and control group(n=15).The retinoic acid gavage method was used to construct rat osteoporosis models.The rats in control group were gavaged with saline.The acid phosphatase (ACP) and alkaline phosphatase (ALP) levels in rat serum in two groups were detected.The expression levels of PTPMEG2 in some rat tissues were detected by Western blotting method.The expression level of PTPMEG2 in bone marrow cells (BMCs),red blood cells and bone marrow stromal cells (BMSCs)were detected and compared.Results The PTPMEG2 polyclonal antibody was successfully prepared.The levels of serum ACP and ALP of rats in model group were higher than those in control group (P<0.01).The expression level of PTPMEG2 in kidney tissue of rats in model group was higher than that in control group(P<0.05),but the expression level in muscle tissue of rats in model group was lower than that in control group(P<0.05).Compared with control group,the expression levels of PTPMEG2 in red blood cells and BMCs of rats in model group has no significant changes,but the expression level of PTPMEG2 in rat BMSCs in model group was significantly increased. Conclusion The expression level of PTPMEG2 in BMCs of osteoporosis rats is increased,and mostly in mature bone cells differentiated in bone marrow;and  PTPMEG2 has important role in osteoporosis development.

Key words: megakaryocyte protein toyrosine phosphatase 2, polyclonal antibody, osteoporosis

中图分类号: 

  • R58