糙皮侧耳碱提水溶性多糖对肿瘤的抑制作用及其机制

J4 ›› 2012, Vol. 38 ›› Issue (6): 1091-1095.

糙皮侧耳碱提水溶性多糖对肿瘤的抑制作用及其机制

孔繁利^1,2,孙新³,佟海滨³|林琪³|刘妍³|李玉林¹

1.吉林大学白求恩医学院病理生物学教育部重点实验室|吉林长春 130021；2.北华大
学基础医学院病理生理学教研室|吉林吉林 132013；3.北华大学生命科学研究中心|
吉林吉林 132013

收稿日期:2012-10-15 发布日期:2012-11-28
通讯作者: 李玉林(Tel:0431-85619481,E-mail:ylli@mail.jlu.edu.cn)； 孙新（Tel:0432-64608351,E-mail:sunxinbh@126.com） E-mail:ylli@mail.jlu.edu.cn;sunxinbh@126.com
作者简介:孔繁利(1974-)|男|吉林省四平市人|副教授|在读医学博士|主要从事肿瘤病理学研究。
基金资助:
吉林省教育厅科研基金资助课题(2011-131，2012-171，2012-124)；吉林省科技厅青年基金资助课题（201201143）

Inhibitory effect of water-soluble polysaccharide alkali-extracted from Pleurotus ostreatus and its mechanism

KONG Fan-li^1,2,SUN Xin³,TONG Hai-bin³,LIN Qi³,LIU Yan³,LI Yu-lin ¹

1.Key Laboratory of Pathobiology,Ministry of Education,Norman Bethune College of Medicine,Jilin University,Changchun 130021,China；2.Department of Pathophysiology,School of Basic Medical Sciences,Beihua University,Jilin 132013,China；3.Research Center of Life Sciences,Beihua University,Jilin 132013,China

Received:2012-10-15 Published:2012-11-28

摘要/Abstract

摘要：

目的：研究糙皮侧耳(Pleurotus ostreatus)碱提水溶性多糖对肿瘤的抑制作用，并探讨其作用机制。方法：通过碱液提取、乙醇沉淀、联合脱蛋白和柱层析方法，分离得到粗皮侧耳碱提水溶性多糖WPOP-N1；BalB/C小鼠前肢腋皮下注射S-180肉瘤细胞建立肿瘤小鼠模型，将60只雌性小鼠随机分为正常对照组、模型对照组、环磷酰胺阳性对照组（30 mg/kg）、WPOP-N1 低、中和高剂量组（100、200和400 mg/kg），每组10 只。除正常对照组外，在每只小鼠的前肢腋皮下注射S-180肉瘤细胞悬液，对照组小鼠给予生理盐水，模型对照组小鼠给予脂多糖10 mg?L-1。采用ELISA法检测各组小鼠血清TNF-α水平，评价WPOP-N1对巨噬细胞吞噬作用，NO和TNF-α水平的影响。结果： WPOP-N1低、中和高剂量组小鼠瘤质量分别为（2.38±0.55）、（1.79±0.64）和（1.37±0.51）g，均低于正常对照组［（3.71±0.81）g］（P<0.05）；WPOP-N1低、中和高剂量组抑瘤率分别为35.8%、51.8%和63.1％；WPOP-N1中、高剂量组荷瘤小鼠血清TNF-α水平高于正常对照组（P<0.05或P<0.01）；腹腔巨噬细胞的吞噬能力高于正常对照组（P<0.05或P<0.01）；WPOP-N1低、中和高剂量组小鼠腹腔巨噬细胞分泌NO和TNF-α水平升高（P<0.05），并具有剂量效应。结论： WPOP-N1具有显著的体内肿瘤抑制作用，其作用机制可能是活化巨噬细胞分泌NO和TNF-α，并增强巨噬细胞的吞噬能力。

关键词: 糙皮侧耳；多糖；免疫调节；巨噬细胞

Abstract:

Objective To study the inhibitory effect of water-soluble polysaccharide alkali-extracted from Pleurotus ostreatus and to clarify its mechanism.Methods The water-soluble polysaccharide WPOP-N1 from Pleurotus ostreatus were obtained by alkaline extraction,ethanol precipitation,and joint deproteinized and column chromatography methods.Forelimbs of BalB/C mice were subcutaneously injected with S-180 sarcoma cells to establish mouse tumor models.60 BalB/C mice were randomly divided into normal control group,model control group,cyclophosphamide (positive control) group,WPOP-N1 low,medium,high doses groups（100,200 and
400 mg/kg）,and there were 10 mice in each group.Besidesnormal control group,the mice in the other groups were subcutaneously injected with S-180 sarcoma cells suspensions,and the mice in normal control group were given saline and the mice in model control group were given lipopolysaccharide.The levels of serum TNF-α secreted were detected by ELISA method.The effects of WPOP-N1 on macrophage phagocytic function and the levels of NO and TNF-α were estimated.Results The tumor weights of mice in WPOP-N1 low,medium and high doses groupswere (2.38±0.55),(1.79±0.64),and (1.37±0.51) g,respectively,which were lower than that in normal control group (3.71 g±0.81 g) (P<0.05);the anti-tumor rates of WPOP-N1 low,medium and high doses groups were 35.8%,51.8%,and 63.1%;the serum TNF-α levels of mice in WPOP-N1 medium and high doses groups were higher than that in normal control group (P<0.05 or P<0.01);the phagocytic activities of peritoneal macrophages of mice in WPOP-N1 medium and high doses groups were higherthan that in normal control group (P<0.05 or P<0.01);the NO and TNF-α levels secreted by murine peritoneal macrophages in WPOP-N1 low,medium and high doses groups were increased (P<0.05) and had a dose-response.Conclusion WPOP-N1 has a significantly inhibitory effect on tumor in vivo and its mechanism may be related to activating macrophages to secrete NO and TNF-α,and enhancing the phagocytic activities of macrophages.

Key words: Pleurotus ostreatus;polysaccharide;immunomodulation;macrophage

中图分类号:

R733

糙皮侧耳碱提水溶性多糖对肿瘤的抑制作用及其机制[J]. J4, 2012, 38(6): 1091-1095.

KONG Fan-li,SUN Xin,TONG Hai-bin,LIN Qi,LIU Yan,LI Yu-lin. Inhibitory effect of water-soluble polysaccharide alkali-extracted from Pleurotus ostreatus and its mechanism[J]. J4, 2012, 38(6): 1091-1095.