双表达重组腺病毒介导的骨形态发生蛋白2-9复合纳米羟基磷灰石/聚酰胺66融合器用于山羊椎间融合的效果评价

J4 ›› 2012, Vol. 38 ›› Issue (6): 1086-1090.

双表达重组腺病毒介导的骨形态发生蛋白2-9复合纳米羟基磷灰石/聚酰胺66融合器用于山羊椎间融合的效果评价

马金梁¹|黄帆¹|汪洋¹|罗进勇²|邓忠良¹

1.重庆医科大学附属第二医院骨科|重庆400010；2.重庆医科大学分子医学实验室|重庆400016

收稿日期:2012-03-03 出版日期:2012-11-28 发布日期:2012-11-28
通讯作者: 邓忠良（Tel：023-63693138，E-mail：deng7586@gmail.com） E-mail:deng7586@gmail.com
作者简介:马金梁(1978-)|男|湖北省荆州市人|讲师|医学博士|主要从事脊柱微创外科方面的研究。

Evaluation on effects of bone morphoge netic protein2-9 mediated by co-expressing recombinant adenovirus combined with nano-hydroxyapatite/polyamide 66 cage  on |lumbar interbody fusion |of goats

MA Jin-liang¹|HUANG Fan¹|WANG Yang¹|LUO Jin-yong²|DENG Zhong-liang¹ 

1.Departments of Orthopedics,Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,China；2.Molecular Medicine Laboratory, Chongqing Medical University|Chongqing 400016|China

Received:2012-03-03 Online:2012-11-28 Published:2012-11-28
Supported by:
国家“863”计划资助课题 (2008AA02Z443)；重庆市卫生局科研基金资助课题(2011-1-053)

摘要/Abstract

摘要：

目的：比较双表达重组腺病毒介导的骨形态发生蛋白2-9（Adv-BMP2-9）与单表达Adv-BMP9和Adv-BMP2复合纳米羟基磷灰石/聚酰胺66（nHA/PA66)融合器用于山羊椎间融合的效果，验证BMP2与BMP9的协同成骨作用。方法：选用40只中国青山羊行L2-3、L3-4和L 4-5腰椎间融合术，共融合120个腰椎间隙。每个腰椎间隙植入nHA/PA66 融合器复合以下4种腺病毒之一，Adv-BMP9（Adv-BMP9组,n=30）、Adv-BMP2 (Adv-BMP2组，n=30)、Adv-BMP2-9（Adv-BMP2-9组,n=30）和Adv-GFP（Adv-GFP组,n=30）。术后24周处死所有山羊，取出融合节段标本，行影像学评估和生物力学分析。结果：X线检测，术前、术后各组间椎间隙高度(DSH）比较差异无统计学意义(P>0.05)；各组标本椎间融合率，Adv-BMP2-9组为70% (21/30)，Adv-BMP9组为53% (16/30)，Adv-BMP2组为43% (13/30)，而Adv-GFP组为30%（9/30）。各组椎间CT融合度评分，Adv-hBMP2-9组、Adv-hBMP9组、Adv-hBMP2组和Adv-GFP组依次为52.29±0.72、44.02±0.67、38.83±0.86和28.65±0.47，各组间比较差异均具有统计学意义(P<0.05或P<0.01)。生物力学评估，Adv-BMP2-9组融合节段刚度大于Adv-BMP9组( P<0.05) ；Adv-BMP9组融合节段刚度大于Adv-BMP2组，但差异无统计意义(P>0.05)。实验期间各组山羊均未观察到局部或远处感染、发热和异物排斥反应。结论：双表达Adv-BMP2-9复合nHA/PA66 融合器对山羊椎间融合效果优于单表达Adv-BMP9或Adv-BMP2，BMP2与BMP9具有协同成骨作用。

关键词:
骨形态发生蛋白；重组腺病毒；椎间融合；纳米羟基磷灰石；聚酰胺

Abstract:

Abstract:Objective To compare the interbody fusion effects of bone morphogenetic protein 2-9 (Adv-BMP 2-9) with single expression of Adv-BMP9 or adv-BMP2 mediated by co-expressing recombinant adenovirus combined with nano-hydroxyapatite/polyamide 66 (nHA/PA66) cage on lumbar interbody fusion of goats,and to clarify the collaborative osteogenesis effects of BMP2 and BMP9.Methods Interbody fusion was performed at L2-3,L3-4 and L4-5 in 40 Chinese green goats,included a total of 120 interspace of lumbar vertebrae.Each interspace of lumbar vertebra was randomly implanted with a nHA/PA66 cage combined with one of the following four adenovirus, Adv-BMP9 (Adv-BMP9 group,n=30),Adv-BMP2 (Adv-BMP2 group,n=30),Adv-BMP2-9 (Adv-BMP2-9 group,n=30),and Adv-GFP (Adv-GFP group,n=30).The goats were euthanized 24 weeks after operation,and the fusion segment speciments were obtained;radiographic inspection and biomechanical analysis were performed on the segment speciments.Results The X-ray results showed there was no significant dirence of disc space height(DSH) before and after operation between various groups.The interbody fusion rates were 70% (21/30) in Adv-BMP2-9 group and 53% (16/30) in Adv-BMP9 group and 31% (5/16) in Adv-BMP2 group and 30% (9/30) in Adv-GFP group.CT fusion degree tests showed that CT fusion scores in Adv-hBMP2-9 group,Adv-hBMP9 group,Adv-hBMP2 group and Adv-GFP group were 52.29±0.72,44.02±0.67,38.83±0.86,and 28.65±0.47,respectively; there were significant differences between various groups(P<0.05 or P<0.01).Biomechanical analysis showed that the stiffness of the fusion segment in Adv-BMP2-9 group was higher than that in Adv-BMP9 group(P<0.05),and stiffness in the fusion segment in Adv-BMP9 group was higher than that in Adv-BMP2 group,but there was no significant difference(P>0.05).During the experiment,no local or distant infection,fever,and foreign body rejection were observed in all goats.Conclusion The interbody fusion effect of co-expressing Adv-BMP2-9 combined with nHA/PA66 cage is superior to Adv-MP9 or Adv-BMP2 on goat lumbar,and BMP2 and BMP9 have collaborative osteogenesis.

Key words: bone morphogenetic protein')">

bone morphogenetic protein, recombinant adenovirus, nano-hydroxyapatite')">interbody fusion; nano-hydroxyapatite, polyamide

中图分类号:

R681.5

马金梁|黄帆|汪洋|罗进勇|邓忠良.
双表达重组腺病毒介导的骨形态发生蛋白2-9复合纳米羟基磷灰石/聚酰胺66融合器用于山羊椎间融合的效果评价[J]. J4, 2012, 38(6): 1086-1090.

MA Jin-liang|HUANG Fan|WANG Yang|LUO Jin-yong|DENG Zhong-liang .

Evaluation on effects of bone morphoge netic protein2-9 mediated by co-expressing recombinant adenovirus combined with nano-hydroxyapatite/polyamide 66 cage  on |lumbar interbody fusion |of goats[J]. J4, 2012, 38(6): 1086-1090.