关键词: 心脏停搏, 人工, 肝移植

Abstract: Objective To build the model of ortholopic liver transplantation (OLT) of heart arrest donor of rat to observe the effects of HSP70 on the liver graft ischemia-reperfusion injury at different time-point after heart arrest. Methods The rats were divided into 4 groups according to the ischemic preconditioning (IP) or not, and the time of heart arrest (45 or 60 min). One hour after portal vein reperfusion,the blood samples were obtained to determine the levels of alanine amino transferase (ALT) and L-asparate amino transferase (AST), grafts samples were obtained for optical microscope observation, and the survival rate of 1 week was observed. Results The HSP70 expressions in IP groups were low at 6 h, and high at 24 h and 48 h. While there were almost no HSP70 expressions in the control group (C group) at 24 h and 48 h.At 1 h after portal vein reperfusion, the levels of ALT and AST in the IP groups (IP-45, IP-60) were significantly lower than those in C groups (C-45, C-60). At one week after the operation, in the IP groups, the structures of the hepatic lobules were very well, there were a little infiltration of lymphocytes in the portal area; while in C group there existed considerable hepatic necrosis and ballooning degeneration. In the IP groups, the survival rates at one week after operation were 62.5% and 37.5%,respectively, while in the C-45 and C-60 groups were 12.5% and 0,respectively. Conclusion HSP70 not only significantly improve liver function and graft pathological changes, but also increase survival rate of rat liver transplantation from heart arrest donors. HSP70 have remarkably protective effects on graft ischemia reperfusion injury in rat liver transplantation from heart arrest donors.

Key words: heart arrest, induced, liver transplantation