乳胞素联合卡铂对卵巢癌SKOV3细胞的增殖抑制作用和促凋亡作用

doi:10.13481/j.1671-587x.20170606

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (06): 1098-1102.doi: 10.13481/j.1671-587x.20170606

乳胞素联合卡铂对卵巢癌SKOV3细胞的增殖抑制作用和促凋亡作用

王雪, 秦瑞, 王皓莹, 高守阳, 兰彦方, 田秀娟

吉林大学中日联谊医院妇产科, 吉林长春 130033

收稿日期:2017-07-03 出版日期:2017-11-28 发布日期:2017-12-01
通讯作者: 田秀娟,教授,主任医师,硕士研究生导师(Tel:0431-84995752,E-mail:349500296@qq.com) E-mail:349500296@qq.com
作者简介:王雪(1986-),女,吉林省公主岭市人,主治医师,医学硕士,主要从事妇科恶性肿瘤临床治疗方面的研究。
基金资助:
吉林省财政厅科研基金资助课题（sczsy201523）

Inhititory effect of proliferation and promotion effect of apoptosis of lactacystin combined with carboplatin on ovarian cancer SKOV3 cells

WANG Xue, QIN Rui, WANG Haoying, GAO Shouyang, LAN Yanfang, TIAN Xiujuan

Department of Gynaecology and Obstetrics, China-Japan Union Hospital, Jilin University, Changchun 130033, China

Received:2017-07-03 Online:2017-11-28 Published:2017-12-01

摘要/Abstract

摘要： 目的：探讨蛋白酶体抑制剂乳胞素（LAC）对体外卵巢癌SKOV3细胞增殖和凋亡的影响，并阐明其作用机制。方法：体外培养卵巢癌SKOV3细胞，采用0、2.5、5.0、10.0和20.0μmol·L^-1 LAC干预SKOV3细胞48 h，5μmol·L^-1 LAC干预SKOV3细胞0、24、48和72 h；将细胞分为对照组（细胞不经药物干预）、LAC组（加入5μmol·L^-1 LAC）、卡铂组（加入10、20、40及80μmol·L^-1卡铂）和LAC联合卡铂组（加入5μmol·L^-1 LAC和10、20、40、80μmol·L^-1卡铂）。采用MTT法和流式细胞术（FCM）检测各组卵巢癌SKOV3细胞的增殖抑制率和凋亡率。结果：MTT检测，随着LAC作用时间的延长和浓度的升高，SKOV3细胞的增殖受到明显抑制，48 h时LAC的半数抑制浓度（IC₅₀）为5.36μmol·L^-1；LAC联合卡铂组细胞的增殖抑制率较相同浓度卡铂组明显升高（P<0.05），卡铂的IC₅₀由58.08μmol·L^-1下降至18.37μmol·L^-1。FCM检测，随着LAC作用时间的延长，SKOV3细胞凋亡率呈升高趋势；与卡铂组和LAC组比较，LAC联合卡铂组SKOV3细胞凋亡率明显升高（P<0.05）。结论：LAC可有效抑制卵巢癌SKOV3细胞的体外增殖且能诱导其凋亡，并可以明显增强卡铂对卵巢癌SKOV3细胞株的增殖抑制作用。

关键词: 卵巢肿瘤, SKOV3细胞, 乳胞素, 卡铂, 蛋白酶体抑制剂

Abstract: Objective:To study the influence of proteasome inhibitor lactacystin(LAC)and carboplatin in proliferation and apoptosis of the human ovarian cancer SKOV3 cells in vitro, and to clarify the mechanisms. Methods:The SKOV3 ovarian cancer cells were cultured in vitro; 0, 2.5, 5.0, 10.0 and 20.0 μmol·L^-1 LAC were used to intervent the SKOV3 cells for 48 h;5 μmol·L^-1 LAC was used to intervent the SKOV3 cells for 0, 24, 48, and 72 h; the SKOV3 cells were divided into control group (treated without medical intervention), LAC group (treated with 5 μmol·L^-1 LAC), carboplatin group(treated with 10, 20, 40 and 80 μmol·L^-1 carboplatin), LAC and carboplatin group(treated with 5 μmol·L^-1 LAC and 10, 20, 40, and 80 μmol·L^-1 carboplatin, respectively).MTT method and FCM were used to detect the inhibitory rates of proliferation and apoptotic rates of the SKOV3 cells in various groups. Results:The MTT test results showed that the proliferation of the SKOV3 cells were inhibited with the prolongation of time and increasing of LAC concentration;the half inhibitory concentration(IC₅₀) of LAC at 48 h was 5.36 μmol·L^-1; compared with carboplatin group, the inhibitory rates of proliferation of SKOV3 cells in LAC and carboplatin groups were significantly increased(P<0.05). The IC₅₀ of carboplatin was dropped from 58.08 μmol·L^-1 to 18.37 μmol·L^-1.The FCM results showed that with the prolongation of treated time of LAC, the apoptotic rates of SKOV3 cells were increased;compared with carboplatin group and LAC group, the apoptotic rate of cells in LAC and carboplatin group was increased(P<0.05). Conclusion:LAC can inhibit the proliferation of the ovarian cancer SKOV3 cells and induce the apoptosis, and LAC can enhance the inhibitory effect of proliferation of carboplatin on the ovarian cancer SKOV3 cells.

Key words: ovarian neoplasms, SKOV3 cells, proteasome inhibitor, lactacystin, carboplatin

中图分类号:

R737.31

王雪, 秦瑞, 王皓莹, 高守阳, 兰彦方, 田秀娟. 乳胞素联合卡铂对卵巢癌SKOV3细胞的增殖抑制作用和促凋亡作用[J]. 吉林大学学报(医学版), 2017, 43(06): 1098-1102.

WANG Xue, QIN Rui, WANG Haoying, GAO Shouyang, LAN Yanfang, TIAN Xiujuan. Inhititory effect of proliferation and promotion effect of apoptosis of lactacystin combined with carboplatin on ovarian cancer SKOV3 cells[J]. Journal of Jilin University Medicine Edition, 2017, 43(06): 1098-1102.

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乳胞素联合卡铂对卵巢癌SKOV3细胞的增殖抑制作用和促凋亡作用

Inhititory effect of proliferation and promotion effect of apoptosis of lactacystin combined with carboplatin on ovarian cancer SKOV3 cells

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