杨梅素对人卵巢癌SKOV3细胞的凋亡诱导作用

doi:10.13481/j.1671-587x.20150504

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (05): 902-906.doi: 10.13481/j.1671-587x.20150504

杨梅素对人卵巢癌SKOV3细胞的凋亡诱导作用

于洋¹, 刘亚萌^1,2,3, 吴少花^1,2,3, 谢奇², 衣丹³, 徐海月^1,2,3, 胡耀狄^1,2,3, 陈君^1,2,3, 徐冶¹

1. 吉林医药学院基础医学院实验中心, 吉林吉林 132013;
2. 吉林大学基础医学院病理生理学系, 吉林长春 130021;
3. 吉林省吉林中西医结合医院体检中心, 吉林吉林 132013

收稿日期:2015-01-27 出版日期:2015-09-28 发布日期:2015-09-29
通讯作者: 徐冶,教授,硕士研究生导师(Tel:0432-64560371,E-mail:xuye_9707@163.com) E-mail:xuye_9707@163.com
作者简介:于洋(1980-),女,吉林省吉林市人,讲师,医学硕士,主要从事卫生毒理学方面的研究。
基金资助:
国家自然科学基金面上项目资助课题(81372793);吉林省教育厅十二五科技项目资助课题(2013361);吉林省大学生创新创业项目资助课题(20141027)

Induction effect of myricetinon apoptosis of human ovarian cancer SKOV3 cells

YU Yang¹, LIU Yameng^1,2,3, WU Shaohua^1,2,3, XIE Qi², YI Dan³, XU Haiyue^1,2,3, HU Yaodi^1,2,3, CHEN Jun^1,2,3, XU Ye¹

1. Experiment Center, School of Medical Sciences, Jilin Medical University, Jilin 132013, China;
2. Department of Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China;
3. Medical Examination Center, Jilin Traditional Chinese and Western Medicine Hospital, Jilin 132013, China

Received:2015-01-27 Online:2015-09-28 Published:2015-09-29

摘要/Abstract

摘要：

目的:观察杨梅素对人卵巢癌SKOV3细胞的生长抑制及凋亡诱导作用,并探讨其可能机制。方法:体外培养人卵巢癌SKOV3细胞,按杨梅素给药浓度将细胞分为对照组和低、中、高浓度杨梅素组,杨梅素给药浓度分别为0、20、40和60 mg·L^-1。MTT法检测各组SKOV3细胞存活率;激光共聚焦显微镜观察各组SKOV3细胞核形态变化并计算异常细胞核比率;免疫荧光法检测各组SKOV3细胞中凋亡相关蛋白-活化半胱氨酸蛋白酶3(active Caspase-3)的表达;Western blotting法检测各组SKOV3细胞中DNA损伤相关蛋白γ-H₂AX的表达水平。结果:与对照组比较,低、中和高浓度杨梅素组SKOV3细胞存活率明显下降(t=-12.35,t=-11.42,t=-10.21,P<0.05);激光共聚焦显微镜下各浓度杨梅素组细胞出现核皱缩等凋亡形态学改变,且细胞中异常细胞核比率高于对照组(t=6.87,t=9.35,t=7.22,P<0.05),高浓度杨梅素组异常细胞核比率高于低、中浓度杨梅素组(t=9.55,t=7.25,P<0.05);随着杨梅素给药浓度的增加,SKOV3细胞中active Caspase-3的表达水平有升高趋势,各浓度杨梅素组SKOV3细胞中γ-H₂AX的表达水平高于对照组(t=5.41,t=3.21,t=6.58,P<0.05)。结论:杨梅素可抑制人卵巢癌SKOV3细胞增殖并诱导其凋亡,DNA双链断裂可能是其促凋亡的机制之一。

关键词: 杨梅素, 卵巢肿瘤, SKOV3细胞, 细胞凋亡, DNA双链断裂

Abstract:

Objective To observe the induction effect of myricetin on the apoptosis and the inhibitory effect on the growth of the human ovarian cancer SKOV3 cells,and to explore the probable mechanisms. Methods The human ovarian cancer SKOV3 cells were cultured in vitro.The cells were divided into control group,low,middle and high concentrations of myricetin groups,which were treated with different concentrations (0,20,40 and 60 mg·L^-1) of myricetin.Then the survival rates of cells were tested by MTT method;Laser scanning confocal microscope was used to observe the morphological changes,and the rates of abnormal necleuses were calculated.The expressions of active Caspase-3 in the SKOV3 cells in various groups were observed by immunofluorescence method.The expression levels of γ-H₂AX in the SKOV3 cells in various groups were detected by Western blotting method. Results Compared with control group,the survival rates of the SKOV3 cells in myricetin groups were significantly decreased(t=-12.35,t=-11.42,t=-10.21,P<0.05).The morphology of nucleuses of SKOV3 cells in various groups had some changes for the cause of apoptosis which were observed by laser scanning confocal microscope,nuclear shrinkage for example.The rates of abnormal nucleuses in low,middle and high concentration of myricetin groups were obviously higher than that in control group(t=6.87,t=9.35,t=7.22,P<0.05),and the rate of abnormal nucleus in high concentration of myricetin group was higher than those in low and middle concentrations of myricetin groups(t=9.55,t=7.25,P<0.05).The expression levels of active Caspase-3 in the SKOV3 cells in myricetin groups had increasing tendency,and the expression levels of γ-H₂AX in the SKOV3 cells in myricetin groups were higher than that in control group(t=5.41,t=3.21,t=6.58,P<0.05). Conclusion Myricetin can inhibit the proliferation of SKOV3 cells and induce the apoptosis;its possible mechanism may be related to DNA double strands break.

Key words: myricetin, ovarian neoplosms, SKOV3 cells, cell apoptosis, DNA double strands break

中图分类号:

R737.3

于洋, 刘亚萌, 吴少花, 谢奇, 衣丹, 徐海月, 胡耀狄, 陈君, 徐冶. 杨梅素对人卵巢癌SKOV3细胞的凋亡诱导作用[J]. 吉林大学学报(医学版), 2015, 41(05): 902-906.

YU Yang, LIU Yameng, WU Shaohua, XIE Qi, YI Dan, XU Haiyue, HU Yaodi, CHEN Jun, XU Ye. Induction effect of myricetinon apoptosis of human ovarian cancer SKOV3 cells[J]. Journal of Jilin University Medicine Edition, 2015, 41(05): 902-906.

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杨梅素对人卵巢癌SKOV3细胞的凋亡诱导作用

Induction effect of myricetinon apoptosis of human ovarian cancer SKOV3 cells

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