关键词: 半胱氨酸, 细胞因子类, 核因子-κB, 疾病模型, 动物

Abstract: Objective To study the relationship between hyperhomocysteic acid(HHcy) and artherosclerosis.Methods 20 male rabbits were randomly divided into control and experiment groups(n=10).HHcy models were made by hypodermical injection of DL-methionine for 7 weeks.After 7 weeks homocysteic acid(Hcy),IL-1β,IL-6,and IL-8 in serum of rabbits in two groups were measured by enzyme linked immunosorbent assay(ELISA).And the pathomorphological changes of aorta were observed with HE staining.The number of NF-κB positive cells in the aorta were counted by immunohistochemical method.Results The levels of Hcy,IL-1β,IL-6 and IL-8 in experiment group were higher than those in control group (P<0.05).Artherosclerosis changes were found in the aorta in experiment group and the number of NF-κB positive cells in the aorta in experiment group were more than those in control group（all P<0.05）.Conclusion The excessive secretion of inflammative factors is one of the mechanisms that HHcy causes artherosclerosis and NF-κB plays an importment mediation role.

Key words: cysteine, cytokines, NF-kappa B, disease model, animal