关键词: 环氧合酶2, 疾病模型, 动物

Abstract: Objective To investigate the expression of cycooxygenase 2(COX2) in myocardial fibrosis and significant.Methods Rat myocardium necrosis model was copyed by isoproterenol(Iso)injection (15 mg•kg^-1).The serum aspartate transaminase (AST),lactate dehydrogenase (LDH),creatine kinase (CK) and creatine kinase isoenzymes (CK-MB) in rats were detected by MD-100 automatic biochemical analyzer. The expression of COX2 protein was analyzed by immunohistochemistry. The expression of COX2 mRNA was analyzed by RT-PCR.Results Compared　 with control group,the levels of serum LDH,CK and CK-MB reached peak at 4 h (P<0.05),AST achieved peak at 6 h (P<0.05). The protein expression of COX2 was increased according with the fibrosis severity.COX2 mRNA arrived peak at 24 h (P<0.05) and began decreased at 48 h,but still higher than normal level (P<0.05).Conclusion COX2 mRNA and protein expression levels are increased significantly in myocardial ischemia and the increasing of COX2 expression has a closed relation with fibrosis,which indicates COX2 may be involved in the process of myocardial fibrosis.

Key words: cyclooxygenase 2, disease model, animal