吸烟相关剂量镉对大鼠心肌的损伤作用及其分子机制

J4 ›› 2011, Vol. 37 ›› Issue (4): 591-595.

吸烟相关剂量镉对大鼠心肌的损伤作用及其分子机制

顾俊莲, 王波, 刘亚男, 邵晨, 郭花, 李晓洁, 贾慧婕, 李馨, 殷迪, 李扬

（吉林大学白求恩医学院病理生理学教研室吉林大学前列腺疾病防治研究中心|吉林长春 130021）

收稿日期:2011-03-25 出版日期:2011-07-28 发布日期:2011-07-28
通讯作者: 李扬 E-mail:（Tel: 0431-85619485,E-mail: lyang@jlu.edu.cn）
作者简介:顾俊莲（1982-）|女|吉林省长春市人|医学硕士|主要从事前列腺肿瘤病理生理方面的研究。
基金资助:
吉林省自然科学基金项目资助课题（201115115）

Effect of smoking-dosage cadmium on myocardial injury in rat and its molecular |mechanism

GU Jun-Lian, WANG Bo, LIU Ya-Nan, SHAO Chen, GUO Hua, LI Xiao-Ji, JIA Hui-Jie, LI Xin, YIN Di, LI Yang

(Department of Pathophysiology,Norman Bethune College of Medicine,Research Center for Prevention and Treatment of Prostate Disease,Jilin University,Changchun 130021,China)

Received:2011-03-25 Online:2011-07-28 Published:2011-07-28

摘要/Abstract

摘要：

目的：建立吸烟相关剂量镉的大鼠模型，研究吸烟相关剂量的镉对大鼠心肌的损伤作用，并进一步探讨其可能的分子机制。方法：建立吸烟相关剂量镉的Wistar大鼠模型作为模型组，同时设立生理盐水对照组，分别于20、36和52周处死大鼠，应用ICP-MS检测心肌组织中镉的蓄积量；绘制脏器系数曲线；生化试剂盒检测心肌组织肌酸激酶（CK）和乳酸脱氢酶（LDH）含量；生化试剂盒检测心肌组织超氧化物歧化酶（SOD）的活性和谷胱甘肽（GSH）、丙二醛（MDA）的含量；病理组织切片观察心肌形态改变；RT-PCR检测凋亡相关基因caspase-3、bcl-2及bax的变化；Western blotting 检测凋亡相关蛋白caspase-3、bax、caspase-9及Fas的变化。结果：与对照组比较，模型组心肌组织镉含量在36和52周蓄积增加，差异有统计学意义（P<0.05）；模型组心肌组织脏器系数在20和36周有略微下降趋势,52周下降明显，差异有统计学意义（P＜0.05）；模型组心肌组织中CK含量在20、36和52周含量均明显增加，差异有统计学意义（P<0.05）；LDH含量在36和52周明显增加，差异有统计学意义（P<0.05）；模型组心肌组织SOD活性分别在36和52周明显下降，差异有统计学意义（P<0.05）；GSH的含量在20、36和52周明显下降，差异有统计学意义（P<0.05）；MDA的含量以20、36和52周明显上升，差异有统计学意义（P<0.05）。电镜观察显示，模型组有心肌细胞紊乱、结缔组织局灶性增生等改变。RT-PCR和Western blotting结果显示，与对照组比较，模型组心肌组织caspase-3、bax和Fas的表达明显增加（P<0.05），bcl-2表达明显减少（P<0.05）。结论：吸烟相关剂量镉通过心肌酶CK和LDH的泄漏，造成心肌组织氧化损伤，对心肌产生毒性作用，并最终导致心肌细胞凋亡的发生。

关键词: 吸烟相关剂量镉；大鼠, Wistar；心脏；细胞凋亡

Abstract:

Abstract:Objective
To establish rat model of smoking-dosage cadmium and study the effect of smoking-dosage cadmium on myocardial injury in rats,and to explore its molecular mechanism.Methods The rat models of smoking-dosage cadium were established as model group,control group was set up at the same time.The rats in 20,36 and 52 weeks were killed,then the contents of cadmium in heart and the changes of heart organ coefficient in rats were investigated.The contents of creatine kinase(CK),lactate dehydrogenase(LDH),glutathion(GSH),malonaldehyde(MDA) and activity of superoxide anion dismutase(SOD) in myocardial tissues were detected.The histopathological changes of myocardium were observed by light microscope with HE staining.RT-PCR was performed to examine the caspase-3,bcl-2 and bax mRNA expressions.Western blotting was used to determine the caspase-3,bax,caspase-9 and Fas protein expressions.Results Compared with control group,the contents of cadmium in model group were increased in 36 and 52 weeks（P<0.05）,the organ coefficient of heart in model group was significantly decreased in 52 weeks（P<0.05）,and the contents of CK and LDH in model group were increased obviously in 20,36 and 52 weeks（P<0.05）,and the activities of SOD in model group were decreased in 36 and 52 weeks（P<0.05）,the contents of GSH in model group were decreased in 20,36 and 52 weeks（P<0.05）,the contents of MDA in model group were increased in 20,36 and 52 weeks（P<0.05）.The myocardial cell disorder and focal hyperplasia of connective tissue were observed in model group by light microscope with HE staining.The results of RT-PCR and Western blotting showed that the expressions of caspase-3,bax and Fas were increased（P<0.05） and the expression of bcl-2 was decreased （P<0.05）.Conclusion Smoking-dosage cadmium can do harm to rat heart through decreasing the cardiac enzymes and oxidative stress,at last result in the apoptosis of myocardial cells.

Key words: smoking-dosage cadmium;rats,Wistar;heart;apoptosis

中图分类号:

R-332

顾俊莲, 王波, 刘亚男, 邵晨, 郭花, 李晓洁, 贾慧婕, 李馨, 殷迪, 李扬. 吸烟相关剂量镉对大鼠心肌的损伤作用及其分子机制[J]. J4, 2011, 37(4): 591-595.

GU Jun-Lian, WANG Bo, LIU Ya-Nan, SHAO Chen, GUO Hua, LI Xiao-Ji, JIA Hui-Jie, LI Xin, YIN Di, LI Yang. Effect of smoking-dosage cadmium on myocardial injury in rat and its molecular |mechanism[J]. J4, 2011, 37(4): 591-595.