关键词: SB203580；caspase-3；细胞凋亡；P38MAPK；信号传导

Abstract:

Abstract:Objective
To investigate the effect of P38MAPK on apoptosis of HSC-T6 cell stimulated by acetaldehyde and the change of P38 protein expression and to provide the basis for clinical treatment of liver fibrosis.Methods Control group(HSC-T6 plus 10% calf serum containing DMEM medium),aldehyde group(acetaldehyde was added based on   control group) and experiment groups(adding inhibitor of P38MAPK SB203580 based on  aldehyde　group,the final concentrations were 4,8,16  μmol·L^-1) were set up in this experiment.The apoptotic rate was detected by FCM,the expression of phosphorylated P38MAPK(P-P38MAPK) was determined by Western blotting,the change of caspase-3 protein level was observed by SABC.Results Compared with control group,the apoptotic rate and caspase-3 positive expression rate of HSC-T6 cells  in  aldehyde  group  were  significantly                     decreased (P<0.05 or P<0.01),the level of P-P38MAPK was increased(P<0.01).After administration of SB203580,the apoptotic rate was increased gradually(P<0.01),the expression of P-P38MAPK was decreased(P<0.01),the positive expression rate of caspase-3 was increased(P<0.01),and all in the does-dependent manner.Conclusion SB203580 could promot the apoptosis of HSC-T6 cells,and the occurrence of apoptosis could be related to phosphorylation of P38MAPK and activation of caspase-3.

Key words: SB203580；caspase-3；apoptosis；P38MAPK;signal transduction