卵黄囊内接种诱导免疫耐受大鼠模型的建立及评价

J4 ›› 2011, Vol. 37 ›› Issue (4): 617-621.

卵黄囊内接种诱导免疫耐受大鼠模型的建立及评价

武双双, 姜振宇, 武英, 谭业辉, 王畅, 姚程

（吉林大学第一医院肿瘤中心|吉林长春 130021）

收稿日期:2010-11-17 出版日期:2011-07-28 发布日期:2010-07-28
通讯作者: 姚程 E-mail:（Tel：0431-88788239，E-mail：yaocheng1956@yahoo.com.cn）
作者简介:［作者简介］武双双（1985-）|女|山东省临沂市人|在读医学硕士|主要从事血液疾病诊治的基础与临床研究。
基金资助:
吉林省科技厅科研基金资助课题（200705142）

Establishment and evaluation of immune tolerant rat model induced by yolk sac inoculation

WU Shuang-Shuang, JIANG Zhen-Yu, WU Ying, TAN Ye-Hui, WANG Chang, YAO Cheng

（Tumor |Center, First Hospital,Jilin University,Changchun 130021,China）

Received:2010-11-17 Online:2011-07-28 Published:2010-07-28

摘要/Abstract

摘要：

目的：建立胚胎期诱导免疫耐受大鼠模型，探讨其能否取代人类白细胞抗原（HLA）配型用于造血干细胞移植。方法：实验动物随机分为诱导耐受组、空白对照组、照射移植组和单纯照射组，每组Wistar大鼠20只，SD大鼠20只。妊娠期SD大鼠胚胎卵黄囊内注射宿主Wistar大鼠外周血单个核细胞，诱导免疫耐受，取成熟子代雄性大鼠作为供体，对接受致死量照射后的宿主雌性大鼠进行骨髓移植，观察移植后大鼠生存期、移植物抗宿主病（GVHD）情况，同时采用皮肤移植观察实体器官植活情况。结果：接受诱导免疫耐受供体大鼠骨髓移植的宿主鼠30 d生存率(86.7%)明显高于对照组(0%)(P<0.01)；其病理检查未见GVHD发生，而在空白对照组，可见GVHD发生,诱导耐受组供体及宿主间皮肤移植成活率明显高于非相关鼠皮肤移植（84.6%和0%）。结论：胚胎期诱导免疫耐受可跨越HLA屏障，从而为造血干细胞移植和实体器官移植提供良好供者。

关键词: 胚胎期；免疫耐受；移植

Abstract:

Abstract:Objective
To establish rat model of immune tolerance induced in embryonic stage and to explore the possibility of replacing for human leukocyte antigen （HLA ）matching in hematopoietic stem cell transplantation.Methods The rats were randomly divided into four groups：induced tolerance group,control group,irradiation-transplantation group and irradiation group.Each group had 20 Wistar rats and 20 SD rats.Host female Wistar rats’ peripheral blood mononuclear cells were injected in yolk sac of SD rats during pregnancy to induce immune tolerance.The host female Wister rats were irradiated with lethal dose and accepted bone marrow transplantation from the induced mature male offspring.The survival and the graft- versus- host disease（GVHD） were observed after transplantation.Meanwhile the skin was used to observe the engraftment of solid organ.Results The 30 d　survival rate of the rats accepted the bone marrow of the immune tolerant donor(86.7%) was significantly longer than that in control group (0%)(P<0.01),and there was no pathological GVHD occurred. While in control group,the incidence of GVHD was clearly visible.The skin engraftment rate between the host and the immune tolerant donor in induced tolerance group was significantly higher than that of non-related rats (84.6% vs 0%).Conclusion The induction of immune tolerance in embryo can cross the HLA barrier and offer a good donor for hematopoietic stem cell transplantation and solid organ transplantation

Key words: embryo;immune tolerance;transplantation

中图分类号:

R-332

武双双, 姜振宇, 武英, 谭业辉, 王畅, 姚程. 卵黄囊内接种诱导免疫耐受大鼠模型的建立及评价[J]. J4, 2011, 37(4): 617-621.

WU Shuang-Shuang, JIANG Zhen-Yu, WU Ying, TAN Ye-Hui, WANG Chang, YAO Cheng. Establishment and evaluation of immune tolerant rat model induced by yolk sac inoculation[J]. J4, 2011, 37(4): 617-621.

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