阻断黏着斑激酶表达对涎腺腺样囊性癌细胞侵袭的影响

J4 ›› 2011, Vol. 37 ›› Issue (3): 452-455.

阻断黏着斑激酶表达对涎腺腺样囊性癌细胞侵袭的影响

张斌¹|孙长伏²|王璐¹|黄汉¹|周小平¹|米磊¹

1.辽宁医学院附属第一医院口腔科|辽宁锦州121000；2.中国医科大学附属口腔医学院口腔颌面外科|辽宁沈阳110001

收稿日期:2010-08-20 出版日期:2011-05-28 发布日期:2011-05-28
通讯作者: 米磊（Tel:0416-4197029，E-mail:3229690@163.com） E-mail:3229690@163.com
作者简介:张斌(1971-)|男|辽宁省锦州市人|副主任医师|医学博士|主要从事涎腺恶性肿瘤治疗的研究。 
基金资助:
辽宁省教育厅高等学校科学研究项目资助课题（L2010315）；辽宁医学院博士科研启动基金项目资助课题（2009）

Influence of blocking FAK expression in invasion of salivary adenoid cystic carcinoma cells

ZHANG Bin¹,SUN Chang-fu²,WANG Lu¹,HUANG Han¹,ZHOU Xiao-ping¹,MI Lei¹

1.Department of Stomatology,First Affiliated Hospital,Liaoning Medical College,Jinzhou 121001,China;2.Department of Oraland Maxillofacial Surgery,College of Stomatology,China Medical University,Shenyang 110001,China

Received:2010-08-20 Online:2011-05-28 Published:2011-05-28

摘要/Abstract

摘要：

目的：研究阻断黏着斑激酶(FAK)的表达对涎腺腺样囊性癌肺高转移性细胞系（SACC-LM）侵袭的影响，并探讨其相关机制。方法：选取处于对数生长期的SACC-LM细胞随机分为空白对照组、DMSO溶媒组和Genistein组，应用Transwell小室通过平均穿膜细胞数观察Genistein 对SACC-LM细胞侵袭的影响；将SACC-LM　细胞分为空白对照组、不同浓度Genistein组及Genistein不同作用时间组，应用Western blotting检测FAK和细胞外信号调节激酶（ERK）的蛋白表达水平。结果：与空白对照组比较，Genistein组平均穿膜细胞数明显减少(P<0.05)，FAK和ERK蛋白表达水平随着抑制剂浓度的增高和作用时间的延长而降低(P<0.05)。结论：阻断FAK表达可降低SACC-LM细胞的侵袭能力；在SACC-LM细胞中，FAK的变化影响了ERK蛋白水平的表达。

关键词: 涎腺腺样囊性癌；侵袭；黏着斑激酶

Abstract:

Abstract:Objective To study the influence of blocking the expression of focal adhesion kinase(FAK) in the invasion behaviorof salivary adenoid cystic carcinoma-lung metastasis（SACC-LM）cells and explore its relevant mechanism.Methods The SACC-LM cells in logarithmic growth phase were selected,and randomly divided into blank control group,DMSO groupand Genistein group,the Transwell chamber was used to observe the influence of Genistein in the invasion of SACC-LM cells.TheSACC-LM cells were divided into blank control group,different doses of Genistein groups and Genistein groups for different treatment time,the expressions of FAK and extracellular signal regulated kinase(ERK) were detected withWestern blotting.Results Compared with bland control group,the invasive ability of SACC-LM cells was reduced and the expressions of FAK andERK protein were decreased with the increase of the inhibitor concentration and prolongation of treatment time (P<0.05) inGenistein groups.Conclusion Blocking FAK expression can reduce the invasion of SACC-LM cells,the changes of FAK affect theexpression of ERK protein in SACC-LM cells.

Key words: salivary adenoid cystic carcinoma;invasion;focal adhesion kinase

中图分类号:

R781.7

张斌|孙长伏|王璐|黄汉|周小平|米磊. 阻断黏着斑激酶表达对涎腺腺样囊性癌细胞侵袭的影响[J]. J4, 2011, 37(3): 452-455.

ZHANG Bin,SUN Chang-fu,WANG Lu,HUANG Han,ZHOU Xiao-ping,MI Lei. Influence of blocking FAK expression in invasion of salivary adenoid cystic carcinoma cells[J]. J4, 2011, 37(3): 452-455.