N-乙酰氨基葡萄糖对乙醇致肝组织损伤的保护作用及其机制

J4 ›› 2011, Vol. 37 ›› Issue (3): 456-459.

N-乙酰氨基葡萄糖对乙醇致肝组织损伤的保护作用及其机制

赵莹¹|吴小兰²|郭林明²|刘艳霞²|刘蕾²|刘俊康²

1.第三军医大学研究生管理大队|重庆 400038;2.第三军医大学天然药物化学教研室|重庆 400038

收稿日期:2010-08-19 出版日期:2011-05-28 发布日期:2011-05-28
通讯作者: 刘俊康(Tel:023-68752190,E-mail:liujunkang@163.com) E-mail:liujunkang@163.com
作者简介:赵莹(1982-)|女|黑龙江省齐齐哈尔市人|在读医学硕士|主要从事天然药物化学研究。
基金资助:
重庆市科委自然科学基金资助课题(2006BB5068)

Protective effect of N-acetylglucosamine on injury of livertissues induced by alcoholic and its mechanism

ZHAO Ying¹,WU Xiao-lan²,GUO Lin-ming²,LIU Yan-xia²,LIU Lei²,LIU Jun-kang²

1. |Administration Office of Graduate Student,Third Military University,Chongqing |400038,China; 2.Department of NaturalProduct Chemistry,Third Military Medical University,Chongqing 400038,China

Received:2010-08-19 Online:2011-05-28 Published:2011-05-28

摘要/Abstract

摘要：

目的：探讨N-乙酰氨基葡萄糖（GlcNAc）减轻乙醇对肝组织损伤的作用，并阐明其作用机制。方法：小鼠灌胃市售白酒建立酒精性肝病模型，同时在市售白酒中加入低、中、高剂量的GlcNAc作为干预组，记录模型组和干预组小鼠开始醉酒时间及醉酒持续时间，HE染色观察模型组和干预组小鼠肠道组织和肝组织的病理损伤程度。结果：与模型组比较，GlcNAc低、中、高剂量组小鼠的开始醉酒时间逐渐延长（P<0.05或P<0.01），醉酒持续时间逐渐缩短（P<0.05或P<0.01）。组织切片观察，模型组小鼠肠道组织结构受损，腺体萎缩，管腔内可见脱落的肠黏膜细胞，肝组织多数细胞胞膜破裂，少许细胞核破、溶解消失，肝小叶中央静脉及其周围肝窦明显扩张，肝细胞索间充满淡红色嗜酸性均质状物，周围组织结构疏松；GlcNAc干预组小鼠肠道及肝组织损伤均有不同程度的减轻，且随着GlcNAc剂量的增加，肠道组织和肝组织损伤逐步减轻。结论：GlcNAc可不同程度减轻乙醇对肠道组织和肝组织的损伤，GlcNAc对肝脏的保护作用可能与GlcNAc稳定肠黏膜屏障作用有关。

关键词: N-乙酰氨基葡萄糖；肠黏膜；通透性；肝细胞/病理学

Abstract:

Abstract:Objective To explore the intervention effect of N-acetylglucosamine(GlcNAc) on injury of liver tissuesinduced by alcohol and clarify its mechanism. Methods Alcohol-induced liver disease model was established by feeding rats with white spirit bought in market. Meanwhile,intervention groups were set up by adding low,middle and high doses of GlcNAc tothe white spirit. Beginning drunken time and drunken duration were then compared between model group and intervention groups. Paraffin section of intestinal tracttissues tissues and liver tissues were used to observe the pathological injury in model group and intervention groups with HEstaining. Results Compared with model group,the beginning drunken time was prolonged (P<0.05,P<0.01) and the drunkenduration was gradually shortened (P<0.05,P<0.01) in intervention groups.The injury of intestinal mucosa structures in modelgroup could observed through paraffin section,the glands shrank back,the exfoliative gut mucosa cells were found. Mostliver cell membranes were disrupted,a few nuclei were broken and disappeared,hepatic cell cord was filled with light-acidophilia and homogeneous matter and adjacent tissues were lossened. There was attenuation at different degrees in all low middle and high doses of GlcNAc groups. Conclusion GlcNAc may relieve the injury of liver tissues and ntestinaltracttissues induced by alcohol,and the protective effect of GlcNAc on liver may be associated with its function of stabilizing the intestinal mucosal barrier.

Key words: alcohol;a-Acetylglucosaming;intestinal mucosa, permeability;hepatocytes/pathology

中图分类号:

赵莹|吴小兰|郭林明|刘艳霞|刘蕾 |刘俊康. N-乙酰氨基葡萄糖对乙醇致肝组织损伤的保护作用及其机制[J]. J4, 2011, 37(3): 456-459.

ZHAO Ying,WU Xiao-lan,GUO Lin-ming,LIU Yan-xia,LIU Lei,LIU Jun-kang. Protective effect of N-acetylglucosamine on injury of livertissues induced by alcoholic and its mechanism[J]. J4, 2011, 37(3): 456-459.

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