多药耐药相关蛋白-2在白血病耐药细胞株K562/A02的表达

J4 ›› 2010, Vol. 36 ›› Issue (2): 312-315.

多药耐药相关蛋白-2在白血病耐药细胞株K562/A02的表达

姜振宇¹,许颖²|荆蕾³|姚程³|马宁¹

1.吉林大学第一医院风湿科|吉林 |长春 |130021；2. 吉林大学第一医院肾病内科|吉林 |长春 |130021；3. 吉林大学第一医院血液肿瘤科|吉林 |长春 |130021

收稿日期:2009-10-09 出版日期:2010-03-28 发布日期:2010-03-28
通讯作者: 姚程(Tel:0431-88782742，E-mail:yaocheng1956@yahoo.com.cn) E-mail:yaocheng1956@yahoo.com.cn
作者简介:姜振宇（1958-）|女|吉林省长春市人|教授|医学博士|主要从事肿瘤耐药的基础与临床研究。
基金资助:
吉林省科技厅科研基金资助课题（200705140）

Expression of multi-drug resistance relative protein-2 in leukemia multi-drug |resistance cell line of K562/A02

JIANG Zhen-Yu¹, XU Ying², JING Lei³, YAO Cheng³, MA Ning¹

1. Department of Rheumatology,First Hospital,Jilin University,Changchun 130021,China;2. Department of Nephrology,First Hospital,Jilin University,Changchun 130021,China;3. Department of Hematology and Oncology,First Hospital,Jilin University,Changchun 130021,China

Received:2009-10-09 Online:2010-03-28 Published:2010-03-28

摘要/Abstract

摘要：

目的:研究多药耐药相关蛋白-2（MRP-2）与白血病细胞耐药的关系，并探讨其在白血病多药耐药中的意义。方法：应用RT-PCR方法检测白血病细胞株K562与耐药细胞株K562/A02中MRP-2 mRNA的差异表达，然后将MRP-2反义及正义寡核苷酸片段分别用脂质体转染至耐药细胞株K562/A02，采用MTT法、流式细胞术及RT-PCR法检测转染后细胞内阿霉素的荧光强度、耐药指数及MRP-2 mRNA的表达情况。结果：耐药细胞株K562/A02中MRP-2 mRNA表达水平明显高于白血病细胞株K562（P<0.05）。MRP-2反义寡核苷酸片段转染后耐药细胞株K562/A02细胞内的阿霉素浓度升高，耐药指数较未转染细胞明显降低（P<0.05）；转染后耐药细胞株K562/A02细胞MRP-2 mRNA表达水平较未转染细胞下降了67.5%，两者比较差异具有显著性（P<0.05）。结论：MRP-2的高表达导致白血病耐药细胞内化疗药物浓度降低，可能是其导致白血病多药耐药的机制之一。

关键词: 白血病；多药耐药；多药耐药相关蛋白-2

Abstract:

Abstract:Objective To investigate the relationship between multi-drug resistance relative protein-2 (MRP-2) and leukemia multi-drug resistance，and study its effect on leukemia multi-drug resistance.Methods The expressions of MRP-2 in leukemia cell line K562 and leukemia multi-drug resistance cell line K562/A02 were detected by PT-PCR.Anti- and oligonucleotide of MRP-2 were transfected by lipidosome into K562/A02,intra-cellular doxorubicin (ADM) fluorescence intensity,resistance index,and the expression of MRP-2 mRNA were detected by MTT,flow cytometry,and RT-PCR.Results The expression of MRP-2 in K562/A02 was higher than that of K562(P<0.05).After transfected with anti-oligonucleotide MRP-2,the ADM fluorescence intensity of K562/A02 was increased,and the resistance index was significantly decreased,and the expression of MRP-2 mRNA was decreased by 67.5% (P<0.05) compared with untransfected cells.Conclusion The high-level expression of MRP-2 results in the reducing of chemical drug concentration,which may be one of the mechanisms of leukemia multi-drug resistance.

Key words: leukemia;multi-drug resistance;multi-drug resistance relative protein-2

中图分类号:

R733.7

姜振宇, 许颖, 荆蕾, 姚程, 马宁. 多药耐药相关蛋白-2在白血病耐药细胞株K562/A02的表达[J]. J4, 2010, 36(2): 312-315.

JIANG Zhen-Yu, XU Ying, JING Lei, YAO Cheng, MA Ning. Expression of multi-drug resistance relative protein-2 in leukemia multi-drug |resistance cell line of K562/A02[J]. J4, 2010, 36(2): 312-315.