TREM-1、TNF-α和IL-8在单核细胞源性泡沫细胞中的表达

J4 ›› 2010, Vol. 36 ›› Issue (2): 316-319.

TREM-1、TNF-α和IL-8在单核细胞源性泡沫细胞中的表达

王宇石¹,刘心刚¹|郑杨²

1.吉林大学第一医院ICU科|吉林长春 130021；2.吉林大学第一医院心内科|吉林长春 130021

收稿日期:2009-11-07 出版日期:2010-03-28 发布日期:2010-03-28
通讯作者: 郑杨（Tel:0431-88782639,E-mail:wysdoctor@yahoo.com.cn） E-mail:wysdoctor@yahoo.com.cn
作者简介:王宇石（1972-）|女|吉林省长春市人|主治医师|医学博士|主要从事危重病医学的研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（05-133）

Expressions of TREM-1,TNF-&alpha|and IL-8 in foam cells derived from macrophages

WANG Yu-SHI¹, LIU Xin-Gang¹, ZHENG Yang²

1.Department of ICU,First Hospital,Jilin University,Changchun 130021,China; 2.Department of Cardiology,First Hospital,Jilin University,Changchun 130021,China

Received:2009-11-07 Online:2010-03-28 Published:2010-03-28

摘要/Abstract

摘要：

目的：探讨髓系细胞触发受体1(TREM-1)、肿瘤坏死因子α(TNF-α)和白细胞介素8(IL-8)在巨噬细胞源性泡沫细胞产生过程中的表达情况。方法：人单核细胞株（U937）经佛波酯（PMA）诱导贴壁后，分为PMA诱导组、低密度脂蛋白（LDL）诱导组及氧化型低密度脂蛋白（ox-LDL）诱导组。应用RT-PCR法检测上述3组细胞中TREM-1 mRNA表达；用ELISA法检测上述3组细胞培养上清中TNF-α和IL-8含量。结果：与PMA诱导组、LDL组细胞比较，ox-LDL组细胞TREM-1 mRNA表达水平均明显增高（P<0.05）；ox-LDL组培养上清中TNF-α、IL-8含量明显高于LDL组及PMA诱导组（P<0.05）。结论：TREM-1、TNF-α和IL-8在动脉粥样硬化（AS）发生发展中可能存在相互诱导、相互协同作用，共同参与AS的形成。

关键词: 泡沫细胞/巨噬细胞；髓系细胞触发受体1;TNF-α；IL-8；动脉粥样硬化

Abstract:

Abstract:Objective To detect the expressions of triggering receptor expressed on myeloid cells-1(TREM-1),TNF-α and IL-8 of foam cells derived from macrophages.Methods Human U937 cell strain was induced by PMA and then was divided into PMA group,LDL group and ox-LDL group.RT-PCR was applied to measure the mRNA expression of TREM-1 in each group and ELISA was used to detect the contents of TNF-α and IL-8 in culture supernatant in each group.Results Compared with PMA group and LDL group,the TREM-1 mRNA expression in ox-LDL group was significantly increased(P<0.05);the TNF-α and IL-8 contents in culture supernatant in ox-LDL group were also significantly increased(P<0.05).Conclusion TNF-α,IL-8,and TREM-1 maybe play an important synergetic effect in the process of atherosclerosis.

Key words: foam cells/macrophages;triggering receptor expressed on myeloid cells-1;TNF-α;IL-8;atherosclerosis

中图分类号:

R541

王宇石, 刘心刚, 郑杨. TREM-1、TNF-α和IL-8在单核细胞源性泡沫细胞中的表达[J]. J4, 2010, 36(2): 316-319.

WANG Yu-SHI, LIU Xin-Gang, ZHENG Yang. Expressions of TREM-1,TNF-&alpha|and IL-8 in foam cells derived from macrophages[J]. J4, 2010, 36(2): 316-319.