J4 ›› 2010, Vol. 36 ›› Issue (4): 741-744.

• 基础研究 • 上一篇    下一篇

HLA-DRB1等位基因与吉林地区汉族人群慢性乙型病毒性肝炎的关联性分析

 韩瑜1, 马宁2, 焦立新1, 林乾飞1, 姜振宇2   

  1. 1.吉林省血液中心输血研究所|吉林 长春 130033;2.吉林大学第一医院风湿病科|吉林 长春 130021
  • 收稿日期:2010-01-05 出版日期:2010-07-28 发布日期:2010-07-28
  • 通讯作者: 姜振宇(Tel:0431-84808382,E-mail:jlujzy@yahoo.com.cn) E-mail:jlujzy@yahoo.com.cn
  • 作者简介:韩 瑜(1976-)|男|吉林省长春市人|主管技师|医学硕士|主要从事人类白细胞抗原遗传方面的研究。
  • 基金资助:

    吉林省科技厅科研基金资助课题(20090454)

Relevance analysis on HLA- DR1 alleles and chronic hepatitis B of Han population in Jilin province

 HAN Yu1, MA Ning2, JIAO Li-Xin1, LIN Gan-Fei1, JIANG Zhen-Yu2   

  1. 1.  |Institute of Blood Transfusion|Jilin Blood Center |Changchun 130033|China;2. Department of Rheumatology|First Hospital|Jilin University|Changchun 130021|China
  • Received:2010-01-05 Online:2010-07-28 Published:2010-07-28

摘要:

目的:探讨HLA-DRB1基因多态性与吉林地区汉族人群慢性乙型肝炎病毒(HBV)感染的相关关系,为慢性乙型病毒性肝炎的治疗和预防提供新思路。方法:采用聚合酶链反应-序列特异性寡核苷酸探针技术(PCR-SSOP)对398例汉族慢性乙型病毒性肝炎患者及838名汉族健康献血者(对照组)进行HLA-DRB1等位基因分型比较。结果:慢性乙型肝炎组HLA-DRB1*03、09等位基因表达频率分别为6.28%和19.60%,明显高于健康对照组(3.58%和12.17%;P=0.031,OR=1.805;P=0.001,OR=1.759);慢性乙型肝炎组HLA-DRB1*04、10和13等位基因表达频率分别为6.28%、0.50%和3.27%,明显低于健康对照组(11.34%、2.39%和5.97%;P=0.005,OR=0.524;P=0.019,OR=0.207;P=0.044,OR=0.523);而所检测HLA-DRB1座位的其他等位基因表达频率与健康对照组比较差异无显著性(P>0.05)。结论:HLA-DRB1*03、09可能是吉林地区汉族慢性乙型病毒性肝炎患者易感基因,HLA-DRB1*04、10、13可能是保护基因。

关键词: 慢性乙型病毒性肝炎;人类白细胞抗原;PCR-SSOP ; 汉族

Abstract:

Abstract:Objective To explore the correlation between the HLA-DRB1 alleles and the chronic hepatitis B of the Han population in Jilin province,and provide a new way for treatment and prevention of chronic hepatitis B.Methods HLA-DRB1 alleles in 398 patients with chronic hepatitis B and 838 healthy controls were analyzed by using the polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) technique.Results The frequencies of HLA-DRB1*03,09 alleles in chronic hepatitis B group were higher than those in healthy control group(6.28% vs  3.58%,P=0.031,OR=1.805; 19.60% vs  12.17%,P=0.001,OR=1.759).The frequencies of HLA-DRB1*04,10 and 13 alleles in chronic hepatitis B group were lower  than those in healthy control group (6.28%  vs  11.34%,P=0.005,OR=0.524;0.50%  vs  2.39%,P=0.019,OR=0.207;3.27%  vs  5.97%,P=0.044,OR=0.523).There were no significant differences in the frequencies of the other HLA-DRB1 alleles between   chronic hepatitis B group and healthy control group(P>0.05).Conclusion The HLA-DRB1*03,09 alleles may be susceptible genes for chronic hepatitis B,and HLA-DRB1*04,10,13 alleles may be protecting gene for chronic hepatitis B of Han population in Jilin province.

Key words: chronic hepatitis B;human leukocyte antigen-DRB1;PCR-SSOP;Han nationality

中图分类号: 

  • R512.6