STAT3与口腔鳞状细胞癌侵袭和转移的关系

J4 ›› 2010, Vol. 36 ›› Issue (6): 1106-1110.

STAT3与口腔鳞状细胞癌侵袭和转移的关系

高扬¹|韩冰²|张茹慧³|王渝⁴|周延民¹

1．吉林大学口腔医院种植中心| 吉林长春 130021 ；2．吉林大学药学院生物化学与分子生物学教研室|吉林长春 130021；3.吉林大学口腔医院口腔颌面外科| 吉林长春 130021； |4. 吉林大学口腔医院病理科|吉林长春 130021

收稿日期:2010-01-20 出版日期:2010-11-28 发布日期:2010-11-28
通讯作者: 周延民 (Tel：0431-88796025,E-mail: gydentist@sina.com ) E-mail:gydentist@sina.com
作者简介:高扬（1980-）|女|吉林省长春市人|在读医学博士|医师|主要从事口腔基础与临床的研究。
基金资助:
吉林省科技厅科研基金资助课题(200705349)

Relationships between oncogene STAT3 and invasion and metastasis of oral |squamous |cell |carcinoma

GAO Yang¹, HAN Bing², ZHANG Ru-Hui³, WANG Yu⁴, ZHOU Yan-Min¹

1. Department of Implant Center,Stomatology Hospital| Jilin University| Changchun 130021|China;2.Department of Biochemistry and Molecular Biology,School of Pharmacy,Jilin University,Changchun |130021,China;3. Department |of Oral and Maxillofacial Surgery,Stomatology Hospital,Jilin University,Changchun 130021,China;4. Department of Pathology,Stomatology Hospital,Jilin University,Changchun 130021,China

Received:2010-01-20 Online:2010-11-28 Published:2010-11-28

摘要/Abstract

摘要：

目的：分析信号转导和转录激活因子-3(STAT3)在口腔鳞状细胞癌组织中的表达情况，探讨其与口腔鳞状细胞癌发生、发展、浸润及转移的关系。方法：采用原位杂交方法(ISH)和免疫组织化学SP法检测60例正常口腔黏膜组织、30例不典型增生组织及60例口腔鳞状细胞癌组织中STAT3 mRNA和蛋白的表达情况，同时结合临床病例资料进行分析。结果： STAT3 mRNA在正常口腔黏膜组织、不典型增生组织及鳞状细胞癌组织中的阳性表达率依次增高，分别为25.0%（15/60）、40.0%（12/30）和68.3%（41/60），组间比较差异有显著性(χ2=23.855,P<0.05）；不同分化程度、不同浸润深度及有无淋巴结转移的口腔鳞状细胞癌组织之间STAT3 mRNA表达比较差异均有显著性 (χ2=15.912,χ2=25.616,χ2=7.455,均P<0.05)。口腔鳞状细胞癌组织中STAT3蛋白表达与癌组织的分化程度、浸润深度及淋巴结转移有密切关联，不同分化等级的鳞状细胞癌组织STAT3蛋白阳性表达率比较差异有显著性(Ⅰ级40.0%，Ⅱ级73.9%，Ⅲ级90.9%；χ2=11.532， P<0.05)；浅层浸润癌组织与深层浸润癌组织的STAT3蛋白阳性表达率比较差异有显著性（浅层26.3%，深层92.7%；χ2=24.989，P<0.05）；发生淋巴结转移的癌组织和未发生淋巴结转移的癌组织STAT3蛋白阳性表达率比较差异也有显著性（发生转移91.3%，未发生转移59.5%；χ2=5.602，P<0.05）。正常黏膜组织、不典型增生组织及鳞状细胞癌组织中STAT3蛋白表达率依次增高，分别为13.3%(8/60)、30.0%(9/30)和71.2%(43/60)，组间比较差异有显著性（χ2=23.855，P<0.05）。结论：口腔鳞状细胞癌组织中STAT3 mRNA和蛋白阳性表达均增高，并与口腔鳞状细胞癌生物学行为密切相关，提示STAT3过表达与口腔鳞状细胞癌的发生、发展有关，STAT3有望成为口腔鳞状细胞癌早期诊断和判断预后的辅助指标。

关键词: 信号转导和转录激活因子-3；口腔鳞状细胞癌；原位杂交；免疫组织化学

Abstract:

Abstract:Objective To explore the expressions of STAT3 mRNA and STAT3 protein and study their correlations with the occurrence,development,invasion,and metastasis of oral squamous cell carcinoma (OSCC). Methods In situ hybridization (ISH) and immunohistochemistry were used to detect the expressions of STAT3 mRNA and STAT3 protein in 60 cases of normal oral mucosa,30 cases of atypical hyperplasia and 60 patients with oral squamous cell carcinoma,at the same time the clinical information was combinated for analysis. Results The positive expression rates of STAT3 mRNA in normal mucosa,atpical hyperplasia and oral squamous cell carcinoma were 25.0% (15/60),40.0% (12/30),68.3% (41/60),respectively;there were significant differences between various groups (χ2 = 23.855,P<0.05). The STAT3 mRNA expressions between different differentiation,different depths of invasion and lymph node metastasis in oral squamous cell carcinoma had significant differences (χ2=15.912，χ2=25.616，χ2=7.455，P<0.05）. The STAT3 protein expression rates were dramaticlly different at different grades of cancer (grade Ⅰ 40.0%,gradeⅡ 73.9%,grade Ⅲ 90.9%;χ2=11.532， P<0.05).The STAT3 protein expression rate was significantly different between shallow infiltraion cancer and deep infiltration cancer（26.3% vs 92.7%，χ2=24.989，P<0.05）. The STAT3 protein expression rate was also dramaticlly different between lymph node metastasis cancer and non-lymph node metastasis cancer (91.3% vs 59.5%，χ2=5.602，P<0.05）.The STAT3 protein expression rates in normal mucosal tissues,atypical hyperplasia and cancer rates were 13.3% (8/60),30.0% (9/30),and 71.2% (43/60),respectively;the differences between various groups were　significant (χ2 = 23.855,P<0.05).The STAT3 protein expression was closely related with　 cancer tissue differentiation,depth of invasion and lymph node metastasis (χ2 = 11.532,χ2 = 24.989,χ2 = 5.602,P<0.05). Conclusion The STAT3 mRNA and protein are over-expressed in oral squamous cell carcinoma tissue and have close correlations with biological behavior,occurrence and development of oral squamous cell carcinoma . STAT3 may be a new assistant indicator for early diagnosis and prognosis judgement of oral squamous cell carcinoma.

Key words: STAT3;oral squamous cell carcinoma;in situ hybridization;immunohistochemistry

中图分类号:

R739.85

高扬, 韩冰, 张茹慧, 王渝, 周延民. STAT3与口腔鳞状细胞癌侵袭和转移的关系[J]. J4, 2010, 36(6): 1106-1110.

GAO Yang, HAN Bing, ZHANG Ru-Hui, WANG Yu, ZHOU Yan-Min. Relationships between oncogene STAT3 and invasion and metastasis of oral |squamous |cell |carcinoma[J]. J4, 2010, 36(6): 1106-1110.