卡介苗热休克蛋白70对肿瘤细胞裂解物免疫原性的影响

J4 ›› 2011, Vol. 37 ›› Issue (1): 68-71.

卡介苗热休克蛋白70对肿瘤细胞裂解物免疫原性的影响

李贺^1,2, 王华³, 吴秀丽³, 卫红飞³, 孙陆果³, 张永胜³, 于永利¹, 王丽颖³, 万敏³

（1. 吉林大学白求恩医学院免疫学教研室|吉林长春 130021；2. 北华大学药学院药理学教研室|吉林吉林 132013；3. 吉林大学白求恩医学院分子生物学教研室|吉林长春 130021）

收稿日期:2010-08-25 出版日期:2011-01-28 发布日期:2011-01-28
通讯作者: 王丽颖 E-mail:wanmin4715@163.com
作者简介:李贺（1977-）|女|吉林省吉林市人|讲师|医学博士|主要从事基因疫苗方面的研究。
基金资助:
吉林省科技厅重大科技发展计划项目资助课题（20060412-2）

Effect of BCG-derived HSP70 on immunogenicity of tumor cell lysate

LI He, WANG Hua^1,2, WU Xiu-Li³, WEI Hong-Fei³, SUN Liu-Guan³, ZHANG Yong-Sheng³, YU Yong-Li¹, WANG Li-Ying³, WU Min³

(1. Department of Immunology,Norman Bethune College of Medicine,Jilin University,Changchun 130021,China;2. Department of Pharmacology,School of Pharmacy,Beihua University,Jilin 132013,China；3. Department of Molecular Biology,Norman Bethune College of Medicine,Jilin University,Changchun China;130021,China)

Received:2010-08-25 Online:2011-01-28 Published:2011-01-28

摘要/Abstract

摘要：

的：观察卡介苗热休克蛋白70（BCGHSP70）对肿瘤细胞裂解物（TCL）免疫原性的影响，为TCL相关肿瘤疫苗的制备提供依据。方法：应用冻融法制备B16黑色素瘤细胞裂解物B16TCL，将BCGHSP70与B16TCL在体外混合制备BCGHSP70-B16TCL。将C57BL/6小鼠随机分为BCGHSP70-B16TCL组、PBS组、BCGHSP70组和B16TCL组，分别于第1和14天皮下免疫BCGHSP70-B16TCL、PBS、BCGHSP70和B16TCL。于末次免疫后第7天处死小鼠，MTT法观察不同免疫组小鼠脾淋巴细胞在B16TCL体外刺激后的增殖情况，计算刺激指数（SI）。结果：应用冻融法能够使B16细胞完全裂解，所制备的B16TCL中含有丰富的蛋白成分。与PBS组、BCGHSP70组和B16TCL组比较，BCGHSP70-B16TCL组免疫小鼠脾脏明显增大；BCGHSP70-B16TCL免疫组SI高于PBS免疫组（P=0.00）、 B16TCL免疫组（P=0.01）和BCGHSP70免疫组（P=0.00）。结论：BCGHSP70　能够增强B16TCL的免疫原性，提示BCGHSP70可能作为有效的免疫佐剂用于TCL相关肿瘤疫苗的研究。

关键词: 卡介苗；热休克蛋白质类；肿瘤细胞裂解物；免疫原性；免疫佐剂

Abstract:

Abstract:Objective
To observe the effect of BCG-derived HSP70（BCGHSP70） on the immunogenicity of tumor cell lysate and provide basis for the preparation of TCL-based tumor vaccines. Methods B16TCL was prepared from B16 melanoma cells by freeze-thaw,then directly mixed with the recombinant BCGHSP70 in vitro to generate BCGHSP70-B16TCL. C57BL/6 mice were randomly divided into 4 groups:BCGHSP70-B16TCL group,PBS group,BCGHSP70 group and B16TCL group. On days 1 and 14,the mice were injected subcutaneously with BCGHSP70-B16TCL,PBS,BCGHSP70 or B16TCL, respectively. On day 21,the mice were scarified and the splenocytes from each group were tested for their proliferative response to the stimulation of B16TCL in vitro by MTT assay,and the stimulation index (SI) of splenocytes was calculated. Results B16 melanoma cells were lysed completely by freeze-thaw,and abundant proteins were observed in the B16TCL. BCGHSP70-B16TCL immunization resulted in a significant increase in the size of spleens. The SI of splenocytes was higher in BCGHSP70-B16TCL group than those in PBS group (P=0.00),B16TCL group (P=0.01) and BCGHSP70 group (P=0.00). Conclusion BCGHSP70 could enhance the immunogenicity of B16TCL,indicating that BCGHSP70 might be used as an effective adjuvant for the TCL-based tumor vaccine.

Key words: BCG；HSP70；tumor cell lysate;immunogenicity;immune adjuvant

中图分类号:

R392.11

李贺, 王华, 吴秀丽, 卫红飞, 孙陆果, 张永胜, 于永利, 王丽颖, 万敏. 卡介苗热休克蛋白70对肿瘤细胞裂解物免疫原性的影响[J]. J4, 2011, 37(1): 68-71.

LI He, WANG Hua, WU Xiu-Li, WEI Hong-Fei, SUN Liu-Guan, ZHANG Yong-Sheng, YU Yong-Li, WANG Li-Ying, WU Min. Effect of BCG-derived HSP70 on immunogenicity of tumor cell lysate[J]. J4, 2011, 37(1): 68-71.