重组人碱性成纤维细胞生长因子促进急性心肌梗死大鼠缺血心肌血管再生

J4 ›› 2009, Vol. 35 ›› Issue (6): 979-979-982.

重组人碱性成纤维细胞生长因子促进急性心肌梗死大鼠缺血心肌血管再生

王晓杰^1,2, 李晶³, 田海山¹, 马吉胜¹, 周鑫¹, 刘孝菊¹, 王艳芳¹, 姚娜¹, 李校堃^1,2

（1.吉林农业大学生物反应器与药物开发教育部工程研究中心|吉林长春 |130118；2.吉林大学公共卫生学院卫生部放射生物学重点实验室|吉林长春 |130021；3.吉林大学基础医学院药理学教研室|吉林长春 |130021）

收稿日期:2009-06-10 出版日期:2009-11-28 发布日期:2009-11-28
通讯作者: 李校堃 E-mail:Tel:0431-84533348,E-mail:xiaokunli@163.net
作者简介:王晓杰(1972-)|女|吉林省长春市人|副研究员|在读医学博士|主要从事新药的研究与开发。
基金资助:
国家自然科学基金资助课题(30870755)；吉林省科技厅科研基金资助课题(20070922)

Effect of recombinant human basic fibroblast growth factor on angiogenesis of acute myocardial infarction in rats

WANG Xiao-Jie^1,2, LI Jing³, TIAN Hai-Shan¹, MA Ji-Qing¹, ZHOU Xin¹, LIU Xiao-Ju¹, WANG Yan-Fang¹, TAO Na¹, LI Jiao-Kun^1,2

1.ME Engineering Research Center of Bioreactor and Pharmaceutical Development,Jilin Agriculture University,Changchun |130118,China；2.Key Laboratory of |Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China；3.Department of Pharmacology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China）

Received:2009-06-10 Online:2009-11-28 Published:2009-11-28

摘要/Abstract

摘要：

目的:研究重组人碱性成纤维细胞生长因子（rhbFGF）对急性心肌梗死大鼠缺血心肌血管再生的作用，探讨通过心肌缺血区小血管再生治疗冠心病的新途径。方法:制备急性心肌梗死大鼠模型，60只大鼠随机分为心肌梗死模型组、rhbFGF高剂量组（50 mg•L-1）、rhbFGF低剂量组（25 mg•L-1）和假手术组；造模前心肌内多点注射rhbFGF。24 h、2周和4周后利用形态学和组织学方法测定各组心肌梗死面积，并进行缺血心肌微血管密度和病理组织学检查。结果：大鼠急性心肌梗死24 h、2 和4周后，rhbFGF高、低剂量组心肌梗死面积缩小，与模型组比较差异有显著性（P<0.05）；2和4周后，rhbFGF高、低剂量组血管生成增加，与模型组及假手术组比较差异有显著性（P<0.01或P<0.05）；24 h、2和4周后，病理切片HE 染色,模型组显示梗死区心肌细胞水肿，心肌纤维断裂，部分心肌细胞溶解,炎细胞浸润；rhbFGF高、低剂量组梗死区亦出现心肌细胞水肿，少见心肌纤维断裂、心肌细胞溶解。结论： rhbFGF有促进缺血心肌血管再生，缩小急性心肌梗死大鼠心肌梗死面积的作用。

关键词: 心肌梗死；重组人碱性成纤维细胞生长因子；血管再生

Abstract:

Abstract:Objective
To investigate the effect of recombinant human basic fibroblast growth factor (rhbFGF) on myocardial angiogenesis in rats with acute myocardial infarction (AMI) and discuss the potential to extend treatment to coronary artery disease patients who are not optimal candidates for conventional methods by therapeutic angiogenesis.Methods Myocardial infraction was induced in rats by using coronary artery ligation.A total of 60 rats were randomly divided into model group,rhbFGF-H group(50 mg•L-1),rhbFGF-L group (25 mg•L-1) and sham group;rhbFGF was directly injected into ischemic myocardium via some points.Morphometric and histological analysis were used to evaluate infarct size,vascular density and myocardial damage at 24 h,2 weeks and 4 weeks.Results The infarct sizes in the rhbFGF-H group and rhbFGF-L group were decreased significantly and lower than those in model group at 24 h,2 weeks and 4 weeks(P<0.05).The capillary densities in the infarct border zone in rhbFGF-H group and rhbFGF-L group were higher than those in model group and sham group at 2 weeks and 4 weeks（P<0.01,P<0.05）.Hematoxylin-Eosine staining result showed that there were cell swelling,cardial musle fibers breakage,a part of myocyte cytolysis and inflammatory infiltration in the infarct border zone in model group at 24 h,2 weeks and 4 weeks
.Although there were cell swelling in rhbFGF-H group and rhbFGF-L group,but few cardial musle fibers breakage and myocyte cytolysis could be seen. Conclusion rhbFGF can promote the angiogenesis and decrease the infarct size in ischemic myocardium of rats with AMI.

Key words: myocardial infarction;recombinant human basic fibroblast growth

中图分类号:

R542.22

王晓杰, 李晶, 田海山, 马吉胜, 周鑫, 刘孝菊, 王艳芳, 姚娜, 李校堃. 重组人碱性成纤维细胞生长因子促进急性心肌梗死大鼠缺血心肌血管再生[J]. J4, 2009, 35(6): 979-979-982.

WANG Xiao-Jie, LI Jing, TIAN Hai-Shan, MA Ji-Qing, ZHOU Xin, LIU Xiao-Ju, WANG Yan-Fang, TAO Na, LI Jiao-Kun. Effect of recombinant human basic fibroblast growth factor on angiogenesis of acute myocardial infarction in rats[J]. J4, 2009, 35(6): 979-979-982.