栀子大黄汤对酒精性脂肪肝大鼠的保护作用

doi:10.13481/j.1671-587x.20170318

摘要/Abstract

摘要： 目的：制备酒精性脂肪肝（AFL）大鼠模型，探讨栀子大黄汤对AFL大鼠的保护作用及其用药剂量。方法：54只SD大鼠分为正常对照组10只和模型对照组44只；模型对照组大鼠采用乙醇灌胃（50%乙醇溶液6.0mL·kg^-1）+高脂饲料的方法制备AFL模型。4周后选取造模成功的40只大鼠随机分为模型组，辛伐他汀组（10.0mg·kg^-1），低、高剂量栀子大黄汤组（所含生药剂量分别为5.0和10.0g·kg^-1）；每组10只。每隔2周测定大鼠体质量和血清总胆固醇（TC）、甘油三酯（TG）、天门冬氨酸氨基转移酶（AST）和谷氨酸氢基转移酶（ALT）水平。给药后第4周称量大鼠体质量和肝脏质量，检测血清和肝组织中TC、TG、AST、ALT、乙醇脱氢酶（ADH）和乙醛脱氢酶（ALDH）水平，检测血清肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）和IL-1β的水平，采用苏木素-伊红（HE）染色观察肝组织病理形态表现并进行病理学检查评分。结果：给药4周后，与正常对照组比较，模型组大鼠血清TC、TG、AST、ALT、TNF-α、IL-6和IL-1β水平明显升高（P<0.05或P<0.01）。给药4周后，与模型组比较，低、高剂量栀子大黄汤组大鼠血清TC、TG、AST和ALT水平降低（P<0.05或P<0.01），高剂量栀子大黄汤组大鼠血清TNF-α、IL-1β和IL-6水平降低（P<0.01），低剂量桅子大黄汤组大鼠血清TNF-α、IL-1β水平降低（P<0.01），低和高剂量栀子大黄汤组及辛伐他汀组大鼠肝组织中ADH和ALDH水平降低（P<0.05或P<0.01）。HE染色，与模型组比较，栀子大黄汤组AFL大鼠肝组织的病理损伤情况明显改善。结论：栀子大黄汤能明显改善由高脂联合酒精饮食引起的肝损伤和肝脏脂肪性病变。

关键词: 炎症, 栀子大黄汤, 酒精性肝损伤, 脂肪肝, 酒精性

Abstract: Objective: To establish the alcoholic fatty liver(AFL) animal models,and to explore the protective effect of Zhi Zi Da Huang Tang (ZZDHT) on the rats with AFL and its dosage. Methods: A total of 54 SD rats were divided into normal control group (n=10) and model control group (n=44).The rats in model control group were given alcohol by lavage (50% ethanol solution 6.0 mL·kg^-1) combined with high-fat feed to establish the rat models of AFL.After 4 weeks,the rats in model control group were randomly divided into model group(treated with water),simvastatin group (10 mg·kg^-1), low and high doses (5.0 and 10.0 g·kg^-1) of ZZDHT groups,and there were 10 rats in each group.Every 2 weeks during the process,the body weights and levels of serum total cholesterol (TC), triglyceride (TG), aspertate aminotransferase (AST), and alanine aminotransferase (ALT) were detected.After 4 weeks,the body weights and liver weights of the rats were detected; the levels of TC,TG,AST,ALT,alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in serum and liver tissue of the rats of were detected;the levels of tumor necrosis factor-alpha (TNF-α),interleukin-6 (IL-6) and interleukin-1β(IL-1β) in serum of the rats were detected; the morphology of liver tissue was observed by HE staining and the pathological examination was performed. Results: Compared with normal control group,the levels of serum TC,AST,ALT, TG,TNF-α,IL-6,and IL-1β of the rats in model group 4 weeks after administration were significantly increased (P<0.05 or P<0.01).Compared with model group, the levels of serum TC,TG,AST,and ALT of the rats in low and high doses of ZZDHT groups 4 weeks after administration were decreased (P<0.05 or P<0.01); the levels of serum TNF-α and IL-6,IL-1β of the rats in high dose of ZZDHT group were decreased (P<0.01);the levels of serum TNF-α and IL-1β of the rats in low dose of ZZDHT group were decreased (P<0.01); the levels of serum ADH and ALDH in liver tissue of the rats in low and high doses of ZZDHT groups and simvastatin group were decreased (P<0.05 or P<0.01).The HE staining result showed that compared with model group, the pathological conditions of the liver tissue of the AFL rats in ZZDHT groups were significantly improved. Conclusion: ZZDHT can significantly improve the liver injury caused by high fat diet combined with alcohol and fat liver lesions.

Key words: Zhi Zi Da Huang Tang, alcoholic liver injury, fatty liver,alcoholic, inflammation

中图分类号:

R285

杨战锋, 李晓勇, 周百中, 陈升阳. 栀子大黄汤对酒精性脂肪肝大鼠的保护作用[J]. 吉林大学学报(医学版), 2017, 43(03): 555-560.

YANG Zhanfeng, LI Xiaoyong, ZHOU Baizhong, CHEN Shengyang. Protective effect of Zhi Zi Da Huang Tang on rats with alcoholic fatty liver[J]. Journal of Jilin University Medicine Edition, 2017, 43(03): 555-560.

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