吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (04): 742-745.doi: 10.13481/j.1671-587x.20160421

• 基础研究 • 上一篇    下一篇

高活性小牛血去蛋白提取物对小鼠酒精性肝损伤的保护作用

石立强, 陈丽娜, 李洪宇, 谢立亚, 糜心雅, 苑广信, 孙晶波, 王曼力, 徐广宇, 韩笑, 赵南晰, 盛瑜, 杜培革, 安丽萍   

  1. 北华大学药学院微生物与生化药学教研室, 吉林 吉林 132013
  • 收稿日期:2016-01-04 发布日期:2016-07-20
  • 通讯作者: 安丽萍,副教授,硕士研究生导师(Tel:0432-64608278,E-mail:alp960@126.com);杜培革,教授,硕士研究生导师(Tel:0432-64608278,E-mail:dupeige2001@126.com) E-mail:alp960@126.com;dupeige2001@126.com
  • 作者简介:石立强(1991-),女,吉林省四平市人,在读医学硕士,主要从事药物分析方面的研究。
  • 基金资助:

    吉林省科技厅科技创新与科技成果转化计划基金资助课题(0150312014ZX);吉林省卫计委科研项目资助课题(2014078);吉林省发改委科研基金资助课题(2014Y103);吉林省科技厅医药产业发展基金资助课题(20140311084YY)

Protective effect of high activity deproteinized extract of calf blood on alcohol liver injury of mice

SHI Liqiang, CHEN Lina, LI Hongyu, XIE Liya, MI Xinya, YUAN Guangxin, SUN Jingbo, WANG Manli, XU Guangyu, HAN Xiao, ZHAO Nanxi, SHENG Yu, DU Peige, AN Liping   

  1. Department of Microbiology and Biochemistry, School of Pharmacy, Beihua University, Jilin 132013, China
  • Received:2016-01-04 Published:2016-07-20

摘要:

目的:观察小牛血去蛋白提取物(DECB)对乙醇所致小鼠肝损伤的保护作用,并初步探讨其作用机制。方法:健康ICR小鼠60只,随机分为对照组、模型组、阳性药物组和低、中、高剂量DECB组,每组10只。腹腔灌胃给药,对照组小鼠给予生理盐水20 mL·kg -1,低、中和高剂量DECB组小鼠分别给予 0.125、0.250和0.500 g·kg-1DECB,阳性药物组小鼠给予护肝片0.63 g·kg -1,每天1次,连续30d,末次给药1 h 后,除对照组外,其他各组小鼠一次性给予50%乙醇14 mL·kg -1,并禁食16 h建立急性酒精性肝损伤模型。测定小鼠的耐受酒精时间和醉酒时间,检测血清丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)活性,检测肝组织中甘油三酯(TG)、谷胱甘肽(GSH)和丙二醛(MDA)水平,并进行肝组织病理切片检查。结果:与模型组比较,各剂量DECB组小鼠醉酒症状明显减轻,高剂量DECB组和阳性药组小鼠酒精耐受时间延长(P < 0.05),醉酒时间缩短(P < 0.05),中、高剂量DECB组小鼠血清ALT和AST活性均明显降低(P < 0.05)。与模型组比较,中、高剂量DECB组和阳性药物组小鼠肝组织中MDA和TG水平明显降低(P < 0.05),GSH水平明显升高(P < 0.05)。高剂量DECB组小鼠乙醇所致肝组织病理学损伤明显减轻。结论:DECB能明显改善乙醇所致肝损伤,其机制可能是通过抑制肝组织氧化应激反应实现的。

关键词: 高活性小牛血去蛋白提取物, 酒精性肝损伤, 氧化应激

Abstract:

Objective: To observe the protective effect of deproteinized extract of calf blood(DECB) on the ethanol-induced liver injury of the mice, and to preliminaryly discuss its mechanism. Methods: Sixty healthy ICR mice were divided into control group, model group, positive drug group,low,medium and high doses of DECB groups (n=10).By intragastric administration, the mice in control group were given 20 mL·kg-1saline solution, the mice in low, medium and high doses of DECB groups were administrated with 0.125,0.250,0.500 g·kg -1 DECB, and the mice in positive drug group were administrated with 0.63 g·kg -1Hugan Tablets;once a day for 30 d. 1 h after the last administration, except control group,the mice in other groups were administrated with one-time grant of 50% ethanol 14 mL·kg -1, and fasted for 16h to establish the models of acute alcohol liver injury. The endurance alcohol time and drunk time of the mice were determined,the activities of aspartate aminotransferase (ALT) and alanine transaminase (AST) activity in serum of the mice were detected, the levels of triglyceride (TG), glutathione (GSH) and malonic dialdehyde (MDA) in liver tissue were determined,and the pathological changes of liver tissue were detected. Results: Compared with model group, the drunk symptoms of the mice in different doses of DECB groups were obviously reduced, the endurance time of the mice in high dose of DECB group and positive drug group was prolonged (P < 0.05), and the drinking time was shortened (P < 0.05);the ALT and AST activities in serum in mediun and high doses of DECB groups were significantly lower than those in model group (P < 0.05).Compared with model group, the MDA and TG levels in liver tissue of the mice in medium and high doses of DECB groups and positive drug group were obviously reduced, and the GSH levels were increased(P < 0.05);compared with model group, the pathological damages of liver tissue of the mice in high dose of DECB group caused by ethanol were significantly reduced. Conclusion: DECB can improve ethanol-induced liver injury which may be related to the inhibition of hepatic oxidative stress response.

Key words: high activity deproteinized extract of calf blood, alcoholic liver injury, oxidative stress

中图分类号: 

  • R575.1