Taurolidine 联合X射线对小鼠恶性黑色素瘤细胞周期进程的影响

J4 ›› 2011, Vol. 37 ›› Issue (1): 21-25.

Taurolidine 联合X射线对小鼠恶性黑色素瘤细胞周期进程的影响

孙宝胜^1,2, 张矛¹, 刘林林³, 张伟静³, 孙娟⁴, 龚守良²

（1. 吉林省肿瘤医院放疗三科|吉林长春130012；2. 吉林大学公共卫生学院　卫生部放射生物学重点实验室|吉林长春 130021；3. 吉林大学第二医院肿瘤生物治疗科|吉林长春 130041；4. 吉林省前卫医院内科|吉林长春130012）

收稿日期:2010-07-05 出版日期:2011-01-28 发布日期:2011-01-28
通讯作者: 刘林林 E-mail:linlinliucn@yahoo.com.cn
作者简介:孙宝胜（1972-）|男|吉林省长春市人,副主任医师|医学博士|主要从事肿瘤放射敏感性、肿瘤细胞凋亡、细胞周期分布及其信号传导等方面研究。

Effects of taurolidine in combination with X-rays irradiation on cell cycle progression of murine melanoma cells

SUN Bao-Sheng^1,2, ZHANG Mao¹, LIU Lin-Lin³, ZHANG Wei-Jing³, SUN Juan⁴, GONG Shou-Liang²

（1. Department of Radiotherapy,Jilin Provincial Tumor Hospital,Changchun 130012,China;2. Key Laboratory of Radiobiology,Ministry of Health,School of Public Health|Jilin University, Changchun 130021,China;3. Department of Biological Therapy,Second Hospital,Jilin University,Changchun 130041,China;4. Department of Internal Medicine,Qianwei Hospital of Jilin Province, Changchun 130012,China)

Received:2010-07-05 Online:2011-01-28 Published:2011-01-28
Supported by:
爱尔兰国立科技发展基金资助课题（2009-2010）

摘要/Abstract

摘要：

目的：观察taurolidine联合X射线照射对小鼠恶性黑色素瘤（B16-4A5和B16-F10）细胞周期进程的影响，探讨其诱导肿瘤细胞凋亡的发生机制。方法：选择B16-4A5和B16-F10细胞系按给药浓度随机分为4组，taurolidine剂量分别为0、25、50和100 μmol.L^-1，同时进行1、2和4 Gy X射线照射，采用流式细胞术检测细胞凋亡率和细胞周期，Western blotting分析cyclin B、cdc2和caspase-3的表达。结果：与25 μmol.L^-1 taurolidine组比较， 50和100 μmol?L-1 taurolidine组诱导B16-4A5和B16-F10细胞发生_G0/_G1期阻滞，细胞数分别升高54.9%、73.7%和36.8%、55.5%（P＜0.05）； 50 μmol.L^-1 taurolidine联合2和4 Gy X射线照射组，细胞G2/M期阻滞消除，细胞数分别降低52.1%、44.2%和59.3%、52.7%（P＜0.05）。与对照组、单纯taurolidine组及单纯照射组比较，联合4 Gy X射线照射组cyclin B和cdc2的表达降低，caspase-3的表达升高（P＜0.05）。结论：taurolidine联合X射线照射可去除G2/M期阻滞，可选择地抑制肿瘤细胞cyclin B和cdc2的表达、增强caspase-3的表达，共同诱导细胞凋亡。

关键词: taurolidine；X射线；细胞周期；细胞分裂周期2；细胞周期蛋白B

Abstract:

Abstract:Objective
To evaluate the effects and mechanisms of taurolidine in combination with X-rays irradiation on cell cycle progress of murine melanoma cells,and provide a new idea of malignant tumor clinical treatment. Methods B16-4A and B16-F10 cells were divided into 4 groups according to the concentrations of taurolidine (0,25,50 and 100 μmol.L^-1 ),respectively and combined with 1,2 and 4 Gy X-rays irradiation. The apoptotic rate and cell cycles were detected by flow cytometry. The expressions of cyclinB,cdc2,and caspase-3 were detected by Western blotting. Results Compared with 25 μmol.L^-1 taurolidine group,the arrest of G2/M phase of B16-4A and B16-F10 cells after treated with 50 and 100 μmol.L^-1 taurolidine was found and the cell numbers were increased by 54.9%,73.7% and 36.8%，55.5%,respectively(P<0.05).The arrest of G2/M phase of B16-4A and B16-F10 cells was also found and the cell numbers were decreased by 52.1%，44.2% and 59.3%，52.7%(P<0.05),respectively, after treated with 50 μmol.L^-1 taurolidine in combination with 2 and 4 Gy X-rays irradiation. Therefore,the increase of caspase 3 expression and the decrease of cyclin B and cdc2 expressions were induced by the combination of 50 μmol.L^-1 taurolidine with 2 and 4 Gy X-rays irradiation compared with control,taurolidine alone and irradiation alone groups.Conclusion Taurolidine in combination with X-rays irradiation can delete the _G2/M arrest,supress the expressions of cyclin B and cdc2,increase the expression of caspase-3 and induce the apoptosis.

Key words: taurolidine;X-rays;cell cycle;cell division cycle 2；cyclin B

中图分类号:

R730.44

孙宝胜, 张矛, 刘林林, 张伟静, 孙娟, 龚守良. Taurolidine 联合X射线对小鼠恶性黑色素瘤细胞周期进程的影响[J]. J4, 2011, 37(1): 21-25.

SUN Bao-Sheng, ZHANG Mao, LIU Lin-Lin, ZHANG Wei-Jing, SUN Juan, GONG Shou-Liang. Effects of taurolidine in combination with X-rays irradiation on cell cycle progression of murine melanoma cells[J]. J4, 2011, 37(1): 21-25.