J4 ›› 2011, Vol. 37 ›› Issue (1): 26-29.

• 基础研究 • 上一篇    下一篇

20(s)-原人参二醇对体外培养Siha细胞caspase-3表达激活的影响

 赵丽晶1, 许多1, 程宏1, 梁作文1, 鲁育铭1, 韩向北1, 郭亚雄1, 赵丽娟1, 董妍2   

  1. 1. 吉林大学白求恩医学院病理生理学教研室| 吉林 长春 130021;2. 美国杜兰大学细胞生物学教研室|美国 新奥尔良 LA 70062)
  • 收稿日期:2010-06-14 出版日期:2011-01-28 发布日期:2011-01-28
  • 通讯作者: 赵丽娟 E-mail:titan2003@sina.com.cn
  • 作者简介:赵丽晶(1974-)|女|吉林省长春市人|讲师|医学博士|主要从事肿瘤防治研究。
  • 基金资助:

    吉林省人民政府人才开发基金资助课题(2006JD02)

Effects of 20(s)-proto-panaxdiol oncaspase-3 expression and activation in Siha cells in vitro

 ZHAO Li-Jing1, XUDuo1, CHENG Hong1, LIANG Zuo-Wen1, LU Yu-Ming1, HAN Xiang-Bei1, GUO Ya-Xiong1, ZHAO Li-Juan1, DONG Yan2   

  1. (1. Department of Pathophysiology,Norman |Bethune College of Medicine,Jilin University,Changchun 130021,China;2. Department of Structural and Cellular Biology,Tulan University,New Orleans LA 70062,USA)
  • Received:2010-06-14 Online:2011-01-28 Published:2011-01-28

摘要:

目的:观察20(s)-原人参二醇(PPD)对体外培养人宫颈癌Siha细胞中caspase-9和caspase-3转录表达的影响,以及caspase-3的活化形式cleaved caspase-3含量的变化,阐明其诱导Siha细胞凋亡的机制。方法:体外培养人宫颈癌Siha细胞,将其分为阴性对照组(乙醇)和实验组(20  μgL-1 PPD),分别用乙醇和PPD处理体外培养的宫颈癌Siha细胞,48 h后流式细胞仪检测细胞的凋亡峰,半定量RT-PCR、Western blotting和免疫细胞化学染色方法检测PPD处理后Siha细胞中caspase-9和caspase-3基因的转录与表达水平。结果:与阴性对照组比较,20  μg?L-1 PPD处理48 h后,Siha细胞凋亡率增加(P<0.05),Siha细胞中caspase-9与caspase-3转录水平升高(P<0.01),表达上调(P<0.01),caspase-3的活化形式cleaved caspase-3含量增加(P<0.01)。细胞免疫化学检测可见实验组细胞出现棕色颗粒。 结论:PPD可促进人宫颈癌Siha细胞凋亡,其机制与激活caspase家族级联反应有关。

关键词: 20(s)-原人参二醇;Siha细胞;细胞凋亡;caspase-3;caspase-9;基因表达

Abstract:

Abstract:Objective
To study the effects of 20(s)-proto-panaxdiol(PPD)on transcription and protein expressions of caspase-9 and caspase-3,and content of cleaved caspase-3 in Siha cells in vitro,and clarify the mechanism of its apootosis action. Methods Siha cells cultivated in vitro were treated with alcohol (negative control group)and 20 μg?L-1 PPD (20  μg.L-1 PPD group),respectively. The apoptosis was detected by FCM 48 h after treatment. The transcription and protein expressions of caspase-9 and caspase-3 in siha cells were analyzed by RT-PCR,Western blotting and immunocytochemical staining. Results Compared with negative control group,after treated with 20 μg?L-1 PPD for 48 h,the apoptotic rate of Siha cells was increased (P<0.05),the transcription and protein expressions of caspase-9 and caspase-3 were up-regulated(P<0.01),and the content of cleaved caspase-3 was increased(P<0.01). Cell immunochemistry detection showed the  brown particles in the experimental  cells in 20  μg?L-1 PPD group. Conclusion PPD may induce the apoptosis of Siha cells,and the mechanism may be related to the up-regulation and activition of caspase family.

Key words: 20(s)-proto-panaxdiol;Siha cells;apoptosis;caspase-3;caspase-9;gene expression

中图分类号: 

  • Q75