雷帕霉素联合阿霉素对肝癌细胞BEL-7402增殖及迁移的影响及其作用机制

J4 ›› 2011, Vol. 37 ›› Issue (1): 30-34.

雷帕霉素联合阿霉素对肝癌细胞BEL-7402增殖及迁移的影响及其作用机制

金立鹏, 王广义, 谢淑丽, 吕国悦

（吉林大学第一医院肝胆胰外科|吉林 |长春 |130021）

收稿日期:2010-07-22 出版日期:2011-01-28 发布日期:2011-01-28
通讯作者: 王广义 E-mail:wgymd@sina.com
作者简介:金立鹏（1983-）|男,吉林省九台市人|医师|医学硕士|主要从事肝胆胰腺外科疾病的基础与临床研究。
基金资助:
吉林省科技厅青年科研基金资助课题（20080162）

Effects of |combination treatment of rapamycin and |adriamycin on proliferation |and migration of hepatoma cell BEL-7402 and their mechanisms

JIN Li-Peng, WANG Guang-Yi, XIE Shu-Li, LU Guo-Yue

(Department of Hepatobiliary and Pancreatic Surgery,First Hospital,Jilin University,Changchun 130021,China)

Received:2010-07-22 Online:2011-01-28 Published:2011-01-28

摘要/Abstract

摘要：

目的：探讨雷帕霉素(RAPA)与阿霉素(ADM)联合应用对肝癌细胞BEL-7402增殖和迁移的影响，阐明其作用机制。方法：选取处于对数生长期肝癌细胞株BEL7402随机分为空白对照组、乙醇溶媒组、RAPA组、ADM组以及联合用药组，采用MTT法检测各组细胞6 d内的增殖情况，采用RT-PCR方法检测cyclin D1和MMP-2 mRNA表达水平，采用Western blotting法检测cyclin D1蛋白表达水平。结果：细胞培养后第3天起，联合用药组细胞增殖速度显著低于RAPA组、ADM组、乙醇溶媒和对照组（P<0.01）。联合用药组cyclin D1 mRNA相对表达量（0.63）明显低于RAPA组（0.77）、ADM组（0.76）和对照组（1.02），联合用药组MMP-2 mRNA相对表达量（0.88）明显低于RAPA组（1.40）、ADM组（1.28）和对照组（1.98），联合用药组cyclin D1蛋白相对表达量（0.33）明显低于RAPA组（0.75）、ADM组（0.74）和对照组（0.92）。结论：RAPA与ADM联合应用可以增强肝癌细胞系BEL-7402对ADM的敏感性，其机制可能与抑制MMP-2和cyclinD1基因的转录及cyclinD1蛋白的表达有关。

关键词: 雷帕霉素；阿霉素；肝癌细胞；药物协同作用；细胞增殖

Abstract:

Abstract:Objective
To investigate the combination effects of rapamycin(RAPA) and adriamycin (ADM) on the proliferation and migration of hepatoma cell BEL-7402，and furthermore analyze the possible mechanisms. Methods The 　hepatoma cell BEL-7402 in logarithmic growth phase were selected as the research object，and were randomly divided into five groups：blank control group，vehicle group，RAPA group ，ADM group and combination group.The cell proliferation in 6 d in five groups was detected by MTT，the cyclin D1 mRNA and MMP-2 mRNA expressions in various groups were detected by RT-PCR，and the cyclin D1 protein expression was detected by Western blotting. Results From the 3rd day to the 6th day of the cell culture，the cell proliferation rate in combination group was lower than those in control，vehicle，RAPA and ADM groups（P<0.01）.The expression of cyclin D1 mRNA in combination group（0.63）was lower than those in control(1.02),RAPA（0.77）and ADM（0.76） groups.The expression of MMP-2 mRNA in combination group（0.88）was lower than those in control(1.98),RAPA（1.40） and ADM （1.28） groups.The expression of cyclin D1 protein in combination group(0.33) was lower than those in control(0.92),RAPA（0.75）and ADM （0.74） groups. Conclusion The combination treatment of RAPA and ADM can improve the sensitivity of hepatoma cell BEL-7402 to ADM,the mechanism perhaps lies in the combination of the two drugs can inhibit the expressions of cyclin D1 mRNA,MMP-2 mRNA and cyclin D1 protein more significantly.

Key words: apamycin； adriamycin；hepatoma cell；coordination function;proliferation

中图分类号:

R735.7

金立鹏, 王广义, 谢淑丽, 吕国悦. 雷帕霉素联合阿霉素对肝癌细胞BEL-7402增殖及迁移的影响及其作用机制[J]. J4, 2011, 37(1): 30-34.

JIN Li-Peng, WANG Guang-Yi, XIE Shu-Li, LU Guo-Yue. Effects of |combination treatment of rapamycin and |adriamycin on proliferation |and migration of hepatoma cell BEL-7402 and their mechanisms[J]. J4, 2011, 37(1): 30-34.

[1]	赵行宇，杨森，周丽霞，那文婷，陆莉，王译擘，吴珏，张巍 . 胡桃醌对肝癌HepG2细胞的增殖抑制及诱导凋亡作用[J]. 吉林大学学报(医学版), 2013, 39(2): 255-258.
[2]	付京\|陈域\|雷海路\|雷团结\|张鹏. 吉西他滨联合树突状细胞对HepG2 细胞增殖的抑制作用[J]. J4, 2011, 37(3): 460-464.
[3]	徐艳玲, 杜海英, 金明华, 刘晓梅, 张龙, 李鹏, 孙志伟. 氯化镉对裸鼠皮下移植人肝癌SMMC-7721细胞的抑制作用及其对线粒体酶的影响[J]. J4, 2011, 37(1): 56-60.
[4]	李静, 朴云峰, 蒋政, 安鼎伟, 金丽君. STAT3基因在人肝癌细胞株HepG2、Bel7402、SMMC7721和肝癌组织中的表达及意义[J]. J4, 2010, 36(3): 531-535.