吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (03): 607-610.doi: 10.13481/j.1671-587x.20170327

• 临床研究 • 上一篇    下一篇

头孢哌酮钠舒巴坦钠对反复下呼吸道感染患者中性粒细胞胞外网状陷阱形成的影响

唐颖, 黄元平, 荆文花, 关英慧   

  1. 吉林大学第一医院呼吸内科, 吉林 长春 130021
  • 收稿日期:2016-08-26 出版日期:2017-05-28 发布日期:2017-06-01
  • 通讯作者: 关英慧,副主任医师,硕士研究生导师(Tel:0431-84808365,E-mail:1186887791@qq.com) E-mail:1186887791@qq.com
  • 作者简介:唐颖(1982-),女,辽宁省沈阳市人,主治医师,医学博士,主要从事呼吸系统疾病免疫机制方面的研究。
  • 基金资助:
    吉林省科技厅科技发展计划项目资助课题(20110489);吉林大学第一医院二部科研专项基金资助课题(B-24)

Effect of sulbactam sodium/cefoperazone sodium on neutrophil extracellular traps of patients with recurrent lower respiratory tract infection

TANG Ying, HUANG Yuanping, JING Wenhua, GUAN Yinghui   

  1. Department of Respiratory Medicine, First Hospital, Jilin University, Changchun 130021, China
  • Received:2016-08-26 Online:2017-05-28 Published:2017-06-01

摘要: 目的:检测反复下呼吸道感染患者外周血中性粒细胞胞外网状陷阱(NETs)的形成,评价头孢哌酮钠舒巴坦钠对NETs形成的影响。方法:选取反复下呼吸道感染患者36例和健康体检者30名作为病例组和健康对照组。采用激光共聚焦显微镜及扫描电镜检测2组研究对象NETs的形成情况,并根据中性粒细胞的形态将NETs的形成情况分成Ⅰ、Ⅱ和Ⅲ级,计数2组研究对象不同级别NETs形成细胞数量;观察头孢哌酮钠舒巴坦钠处理前后病例组患者NETs的形成情况。结果:病例组患者Ⅰ和Ⅱ级NETs形成细胞数量明显多于健康对照组(P<0.05),而Ⅲ级NETs形成细胞数量少于健康对照组(P<0.05);头孢哌酮钠舒巴坦钠处理后病例组患者Ⅰ和Ⅱ级NETs形成细胞数量明显少于用药前(P<0.05),而Ⅲ级NETs形成细胞数量多于用药前(P<0.05)。结论:反复下呼吸道感染患者体内中性粒细胞能形成大量具有高效抗菌功能的NETs,而头孢哌酮钠舒巴坦钠可以抑制NETs的形成。

关键词: 头孢哌酮钠舒巴坦钠, 扫描电镜, 激光共聚焦显微镜, 反复下呼吸道感染, 中性粒细胞胞外网状陷阱

Abstract: Objective: To detect the neutrophil extracellular traps (NETs) formation in the peripheral blood of the patients with recurrent respiratory tract infection, and to evaluate the effect of sulbactam sodium/cefoperazone sodium on the formation of NETs. Methods: A total of 36 patients with recurrent respiratory tract infection (case group) and 30 healthy volunteers (healthy control group) were selected. The NETs formation of subjects in two groups was detected by confocal microscope and scanning electron microscope (SEM). According to the appearance of neutrophils, the formation of NETs was classified as grade Ⅰ, Ⅱ and Ⅲ,the number of NETs formation cells of subjects in two groups was calculated. The formation of NETs of the patients in case group were detected before and after treated with sulbactam sodium/cefoperazone sodium. Results: The number of NETs formation cells of grade Ⅰ and Ⅱ of the patients in case group was more than that in healthy control group (P<0.05); while the number of NETs formation cells of grade Ⅲ of the patients in case group was less than that in healthy control group (P<0.05). The number of NETs formation cells of grade Ⅰ and Ⅱ of the patients in case group were significantly decreased (P<0.05), while the number of NETs formation cells of grade Ⅲ was significantly increased (P<0.05) after treated with sulbactam sodium/cefoperazone sodium. Conclusion: A lot of NETs with high antibacterial function can be formed in the patients with recurrent lower respiratory tract infection, and sulbactam sodium/cefoperazone sodium can inhibit the formation of NETs.

Key words: recurrent lower respiratory tract infection, confocal microscope, scanning electron microscope, neutrophil extracellular traps, sulbactam sodium/cefoperazone sodium

中图分类号: 

  • R563